晶体色氨酸和包膜色氨酸对幼建鲤生长性能、蛋白质代谢及消化吸收能力影响的比较

唐凌; 孙崇岩; 邝声耀; 冯琳; 周小秋

doi:10.3969/j.issn.1006-267x.2014.02.017

动物营养学报 >

2014 , Vol. 26 >Issue 2: 411 - 419

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.017

水产营养 Aquaculture Nutrition

晶体色氨酸和包膜色氨酸对幼建鲤生长性能、蛋白质代谢及消化吸收能力影响的比较

唐凌 ,
孙崇岩 ,
邝声耀 ,
冯琳 ,
周小秋

展开

1. 四川农业大学动物营养研究所, 温江 611130;
2. 四川省畜牧科学研究院动物营养研究所, 成都 610099;
3. 鱼类营养与安全生产四川省高校重点实验室, 雅安 625014

收稿日期: 2013-08-07

网络出版日期: 2014-01-27

基金资助

公益性行业（农业）科研专项（201003020）资助

收起

Comparison of Effects of Crystalline Tryptophane and Coated Tryptophane on Growth Performance, Protein Metabolism and Abilities of Digestion and Absorption of Juvenile Jian Carp (Cyprinus carpio var. Jian)

TANG Ling ,
SUN Chongyan ,
KUANG Shengyao ,
FENG Lin ,
ZHOU Xiaoqiu

Expand

1. Animal Nutrition Institute of Sichuan Agriculture University, Wenjiang 611130, China;
2. Animal Nutrition Institute of Sichuan Animal Science Institute, Chengdu 610099, China;
3. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Ya'an 625014, China

Received date: 2013-08-07

Online published: 2014-01-27

Fold

摘要

本试验旨在比较晶体色氨酸和包膜色氨酸对幼建鲤生长性能、蛋白质代谢及消化吸收能力的影响。试验选择初始体重为（7.73±0.03） g的健康幼建鲤300尾，随机分成2组（每组设3个重复，每个重复50尾），分别饲喂含晶体色氨酸和包膜色氨酸的试验饲料（色氨酸含量均为0.25%），进行为期60 d的生长试验。结果表明：包膜色氨酸组的特定生长率、蛋白质效率、蛋白质沉积率、脂肪沉积率均显著高于晶体色氨酸组（P<0.05），而饲料系数则显著低于晶体色氨酸组（P<0.05）。与晶体色氨酸相比，包膜色氨酸显著提高了肠长指数、肠体指数、肝体指数、肠道蛋白质含量、肝胰脏蛋白质含量以及前肠、中肠、后肠皱襞高度（P<0.05）；显著提高了肝胰脏和肠道胰蛋白酶和脂肪酶活力（P<0.05）；显著提高了中肠、后肠Na⁺，K⁺-ATP酶和γ-谷氨酰转肽酶（γ-GT）以及前肠、中肠、后肠碱性磷酸酶（AKP）活力（P<0.05）。此外，包膜色氨酸组的血浆氨浓度、肝胰脏和肌肉谷丙转氨酶（GPT）和谷草转氨酶（GOT）活力均显著低于晶体色氨酸组（P<0.05）。色氨酸体外释放速率结果表明晶体色氨酸和包膜色氨酸分别在15和180 min时释放完全。由此可见，与晶体色氨酸相比，包膜色氨酸体外释放速率较低，能更有效地提高幼建鲤的生长性能和消化吸收能力，有利于其体蛋白质的合成。

关键词： 幼建鲤; 晶体色氨酸; 包膜色氨酸; 生长; 消化酶; 刷状缘酶

本文引用格式

唐凌 , 孙崇岩 , 邝声耀 , 冯琳 , 周小秋 . 晶体色氨酸和包膜色氨酸对幼建鲤生长性能、蛋白质代谢及消化吸收能力影响的比较[J]. 动物营养学报, 2014 , 26(2) : 411 -419 . DOI: 10.3969/j.issn.1006-267x.2014.02.017

Abstract

This experiment was conducted to compare the effects of crystalline tryptophane and coated tryptophane on growth performance, protein metabolism and the abilities of digestion and absorption of juvenile Jian carp (Cyprinus carpio var. Jian). A total of 300 juvenile Jian carp with an initial body weight of (7.73±0.03) g were randomly divided into 2 groups (with 3 replicates per group and 50 fish per replicate), and the fish in the 2 groups had been fed experimental diets containing crystalline tryptophane and coated tryptophane for 60 days, respectively. The tryptophane content was 0.25% both for 2 experimental diets. The results showed as follows: the specific growth rate, protein efficiency ratio, protein retention rate and lipid retention rate in coated tryptophane group were significantly higher and the feed conversion ratio in coated tryptophane group was significantly lower than those in crystalline tryptophane group (P<0.05). Compared with the crystalline tryptophane, the coated tryptophane significantly improved the intestosomatic index, intestinal length index, hepatosomatic index, intestinal protein content, hepatopancreas protein content and the fold heights of anterior intestine, middle intestine and posterior intestine (P<0.05); significantly increased the activities of trypsin and lipase in intestine and hepatopancreas (P<0.05); significantly increased the activities of Na⁺,K⁺-ATPase and γ-glutamyl transpeptidase (γ-GT) in middle intestine and posterior intestine and alkaline phosphatase (AKP) in anterior intestine, middle intestine and posterior intestine (P<0.05). Moreover, the concentration of plasma ammonia and the activities of glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) in hepatopancreas and muscle in coated tryptophane group were significantly lower than those in crystalline tryptophane group (P<0.05). The results of release rate of tryptophan in vitro showed that crystalline tryptophane and coated tryptophane were all released at 15 and 180 min, respectively. In conclusion, compared with the crystalline tryptophane, the coated tryptophane has a lower release rate; therefore, it can improve the growth performance and the abilities of digestion and absorption more effectively, which is beneficial to protein synthesis of juvenile Jian carp.

Key words： Cyprinus carpio var. Jian; crystalline tryptophan; coated tryptophan; growth; digestive enzyme; brush border enzyme

参考文献

[1] NOSE T.Summary report on the requirements of essential amino acids for carp[M]//TIEWS K, HALVER J E.Finfish nutrition and fishfeed technology.Berlin:OAI, 1979:145-156.

[2] COSOSO R M, TIRO L B, BENITEZ L V.Requirement for tryptophan by milkfish (Chanos chanos Forsskal) juveniles[J].Fish Physiology and Biochemistry, 1992, 10(1):35-41.

[3] 涂永锋, 叶元土, 宋代军, 等.游离异亮氨酸对鲫鱼的促生长作用[J].饲料工业, 2004, 25(8):52-55.

[4] 王冠, 冷向军, 李小勤, 等.饲料中添加包膜氨基酸对异育银鲫生长和体成分的影响[J].上海水产大学学报, 2006, 15(3):365-369.

[5] BOGUT I, ADAMKOVA I, RASTIJA T, et al.Effect of additive lysine to fodder on the growth of carp fry (Cyprinus carpio) and protein conservation[J].Krmiva, 2000, 42(2):59-64.

[6] AOKI H, AKIMOTO A, WATANABE T.Periodical changes of plasma free amino acid levels and feed digesta in yellowtail after feeding non-fishmeal diets with or without supplemental crystalline amino acids[J].Fisheries Science, 2001, 67(4):614-618.

[7] 李爱杰.水产动物营养与饲料学[M].北京:中国农业出版社, 1996.

[8] TESHIMA S, KANAZAWA A, YAMASHITA M.Dietary value of several proteins and supplemental amino acids for larvae of the prawn Penaeus japonicus[J].Aquaculture, 1986, 51(3/4):225-235.

[9] WILSON R P, ALLEN O W, Jr, ROBINSON E H, et al.Tryptophan and threonine requirements of fingerling channel catfish[J].The Journal of Nutrition, 1978, 108(10):1595-1599.

[10] HE W, ZHOU X Q, FENG L, et al.Dietary pyridoxine requirement of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Nutrition, 2009, 15(4):402-408.

[11] WEN Z P, ZHOU X Q, FENG L, et al.Effect of dietary pantothenic acid supplement on growth, body composition and intestinal enzyme activities of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Nutrition, 2009, 15(5):470-476.

[12] JIANG W D, ZHOU X Q, FENG L, et al.Growth, digestive capacity and intestinal microflora of juvenile Jian carp (Cyprinus carpio var.Jian) fed graded levels of dietary inositol[J].Aquaculture Research, 2009, 40(8):955-962.

[13] LI W, ZHOU X Q, FENG L, et al.Effect of dietary riboflavin on growth, feed utilization, body composition and intestinal enzyme activities of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Nutrition, 2010, 16(2):137-143.

[14] AOAC.Official methods of analysis of AOAC international[S].16th ed.Gaithersburg, MD:AOAC International, 1998.

[15] LIN Y, ZHOU X Q.Dietary glutamine supplementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture, 2006, 256(1/2/3/4):389-394.

[16] HUMMEL B C W.A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin[J].Canadian Journal of Physiology and Pharmacology, 1959, 37:1393-1399.

[17] FURNÉ M, HIDALGO M C, LPEA A, et al.Digestive enzyme activities in Adriatic sturgeon Acipenser naccarⅡ and rainbow trout Oncorhynchus mykiss.A comparative study[J].Aquaculture, 2005, 250(1/2):391-398.

[18] BESSEY O A, LOWRY O H, BROCK M J.Rapid coloric method for determination of alkaline phosphatase in five cubic millimeters of serum[J].Journal of Biological Chemistry, 1946, 164:321-329.

[19] ROSALKI S B, RAU D, LEHMANN D, et al.Determination of serum gamma-glutamyl transpeptidase activity and its clinical applications[J].Annals of Clinical Biochemistry, 1970, 7:143-147.

[20] MCCORMIA S D.Methods for nonlethal gill biopsy and measurement of Na⁺, K⁺-ATPase[J].Canadian Journal of Fisheries and Aquatic Sciences, 1993, 50(3):656-658.

[21] LÓPEZ-ALVARADO J, LANGDON C J, TESHIMA S I, et al.Effects of coating and encapsulation of crystalline amino acids on leaching in larval feeds[J].Aquaculture, 1994, 122(4):335-346.

[22] ZHOU X Q, ZHAO C R, LIN Y.Compare the effect of diet supplementation with uncoated or coated lysine on juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Nutrition, 2007, 13(6):457-461.

[23] 冯琳, 彭艳, 刘杨, 等.晶体苏氨酸和微囊苏氨酸对幼建鲤生长性能和消化吸收能力影响的比较研究[J].动物营养学报, 2011, 23(5):771-780.

[24] ALAM M S, TESHIMA S, KOSHIO S, et al.Effects of supplementation of coated crystalline amino acids on growth performance and body composition of juvenile kuruma shrimp Marsupenaeus japonicus[J].Aquaculture Nutrition, 2004, 10(5):309-316.

[25] 帅柯.蛋氨酸对幼建鲤消化功能和免疫功能的影响[D].硕士学位论文.雅安:四川农业大学, 2006.

[26] BERGE G E, SVEIER H, LIED E.Nutrition of Atlantic salmon (Salmo salar):the requirement and metabolic effect of lysine[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 1998, 120(3):477-485.

[27] TANG L, WANG G H, ZHOU X Q, et al.Effect of methionine on intestinal enzymes activities, microflora and humoral immune of juvenile Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Nutrition, 2009, 15(5):477-480.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们