动物营养学报 >

2014 , Vol. 26 >Issue 2: 526 - 532

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.031

研究简报 Short Communications

果糖和胰岛素对鹅胚原代肝细胞硬脂酰辅酶A去饱和酶1基因表达量的影响

  • 张宜辉 ,
  • 张蕊 ,
  • 张军 ,
  • 王洪荣 ,
  • 龚道清
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  • 扬州大学动物科学与技术学院, 扬州 225009

收稿日期: 2013-09-02

  网络出版日期: 2014-01-27

基金资助

江苏省研究生科研创新计划项目（CXZZ12_0912）；国家自然科学基金项目（31072021）

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Effects of Fructose and Insulin on Stearyl-Coenzyme A Desaturase 1 Gene Expression Level in Primary Germinal Hepatocytes of Geese

  • ZHANG Yihui ,
  • ZHANG Rui ,
  • ZHANG Jun ,
  • WANG Hongrong ,
  • GONG Daoqing
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2013-09-02

  Online published: 2014-01-27

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摘要

本试验旨在研究果糖和胰岛素对鹅胚原代肝细胞硬脂酰辅酶A去饱和酶1（SCD1）基因表达量的影响。以鹅胚原代肝细胞为试验对象，研究不同浓度果糖（0、10、30、50 mmol/L）、胰岛素（0、50、100、200 nmol/L）以及两者协同作用（10 mmol/L果糖+50 nmol/L胰岛素、10 mmol/L果糖+200 nmol/L胰岛素、50 mmol/L果糖+50 nmol/L胰岛素、50 mmol/L果糖+200 nmol/L胰岛素）对鹅胚原代肝细胞SCD1基因表达量的影响。结果表明：10 mmol/L果糖能够极显著上调鹅胚原代肝细胞SCD1基因表达量（P<0.01），30、50、100 mmol/L果糖对SCD1基因表达量均无显著影响（P>0.05）；200 nmol/L胰岛素能够显著上调SCD1基因表达量（P<0.05）；10 mmol/L果糖和200 nmol/L胰岛素协同作用能极显著上调SCD1基因表达量（P<0.01）。结果提示，适宜的果糖、胰岛素单独添加以及协同作用均能上调鹅胚原代肝细胞SCD1基因表达量。

关键词： 果糖; 胰岛素; 鹅胚原代细胞; SCD1

本文引用格式

张宜辉 , 张蕊 , 张军 , 王洪荣 , 龚道清 . 果糖和胰岛素对鹅胚原代肝细胞硬脂酰辅酶A去饱和酶1基因表达量的影响[J]. 动物营养学报, 2014 , 26(2) : 526 -532 . DOI: 10.3969/j.issn.1006-267x.2014.02.031

Abstract

The aim of this study was to detect the effects of fructose and insulin on stearyl-coenzyme A desaturase 1 (SCD1) gene expression level in primary germinal hepatocytes of geese. Primary germinal hepatocytes of geese were used as test object, the effects of different concentrations of fructose (0, 10, 30 and 50 mmol/L), insulin (0, 50, 100 and 200 nmol/L) and their combination (10 mmol/L fructose+50 nmol/L insulin, 10 mmol/L fructose+200 nmol/L insulin, 50 mmol/L fructose+50 nmol/L insulin and 50 mmol/L fructose+200 nmol/L insulin) on the SCD1 gene expression level in primary germinal hepatocytes of geese were detected. The results showed as follows: 10 mmol/L fructose could significantly up-regulate SCD1 gene expression level (P<0.01), and 30, 50 and 100 mmol/L fructose had no significant effect on it (P>0.05); 200 nmol/L insulin could significantly up-regulate SCD1 gene expression level (P<0.05); 10 mmol/L fructose and 200 nmol/L insulin had significantly synergistic effect on the up-regulation of SCD1 gene expression level. The results indicate that fructose and insulin alone supplementation and the combination at proper levels can up-regulate SCD1 gene expression level in primary germinal hepatocytes of geese.

Key words： fructose; insulin; primary germinal hepatocytes of geese; SCD1

参考文献

[1] ENOCH H G, CATALÁ A, STRITTAMTTER P.Mechanism of rat liver microsomal stearyl-CoA desaturase.Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid[J].Journal of Biological Chemistry, 1976, 251(16):5095-5103.

[2] DOBRZYN A, NTAMBI J M.The role of stearoyl-CoA desaturase in the control of metabolism[J].Prostaglandins, Leukotrienes, and Essential Fatty Acids, 2005, 73(1):35-41.  

[3] FLOWERS M T, NTAMBI J M.Role of stearoyl-coenzyme A desaturase in regulating lipid metabolism[J].Current Opinion in Lipidology, 2008, 19(3):248-256.  

[4] NTAMBI J M.Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol[J].Journal of Lipid Reseach, 1999, 40(9):1549-1558.

[5] MZIAUT H, KORZA G, OZOLS J.The N terminus of microsomal delta 9 stearoyl-CoA desaturase contains the sequence determinant for its rapid degradation[J].Proceedings of the National Academy of Sciences of the United States of America, 2000, 97(16):8883-8888.  

[6] KATO H, SAKAKI K, MIHARA K.Ubiquitin-proteasome-dependent degradation of mammalian ER stearoyl-CoA desaturase[J].Journal of Cell Science, 2006, 119:2342-2353.

[7] 蒋越, 高运臻, 何侃, 等.遗传与营养因素对猪LDLR与SCD基因表达的影响[J].上海交通大学学报:农业科学版, 2010, 28(4):315-318.

[8] 陈才, 屈小丹, 富俊才.葵花籽对羊组织共轭亚油酸沉积及相关酶基因表达的影响[J].中国农业大学学报, 2012, 17(1):110-118.

[9] NTAMBI J M.Dietary regulation of stearoyl-CoA desaturase 1 gene expression in mouse liver[J].Journal of Biological Chemistry, 1992, 267(15):10925-10930.

[10] BRYZGALOVA G, LUNDHOLM L, PORTWOOD N, et al.Mechanisms of antidiabetogenic and body weight-lowering effects of estrogen in high-fat diet-fed mice[J].American Journal of Physiology-endocrinology and Metabolism, 2008, 295(4):E904-E912.

[11] COHEN P, MIYAZAKI M, SOCCI N D, et al.Role for stearoyl-CoA desturase-1 inleptin-mediated weight loss[J].Science, 2002, 297:240-243.

[12] FLOWERS M T, KELLER M P, CHOI Y, et al.Liver gene expression analysis reveals endoplasmic reticulum stress and metabolic dysfunction in SCD1-deficient mice fed a very low-fat diet[J].Physiological Genomics, 2008, 33(3):361-372.  

[13] MIYAZAKI M, DOBRZYN A, MAN W C, et al.Stearoyl-CoA desaturase 1 gene expression is necessary for fructose-mediated induction of lipogenic gene expression by sterol regulatory element-binding protein-1c-dependent and-independent mechanisms[J].Journal of Biological Chemistry, 2004, 279(24):25164-25171.  

[14] KIM J B, SARRAF P, WRIGHT M, et al.Nutritional and insulin regulation of fatty acid synthetase and leptin gene expression through ADD1/SREBP1[J].Journal of Clinical Investigation, 1998, 101(1):1-9.  

[15] 洪胜辉, 张军, 邵丹, 等.鹅肝细胞的高纯分离及培养[J].中国兽医学报, 2012, 32(9):1378-1380, 1385.

[16] WATERS K M, NTAMBI J M.Insulin and dietary fructose induce stearoyl-CoA desaturase 1 gene expression of diabetic mice[J].Journal of Biological Chemistry, 1994, 269(44):27773-2777.

[17] 任路平, 宋光耀, 章冬梅, 等.短期高果糖喂养对小鼠肝脏脂质沉积和胰岛素敏感性的影响[J].中国病理生理杂志, 2011, 27(12):2286-2290.

[18] EMERY R S.Biosynthesis of milk fat[J].Journal of Dairy Science, 1973, 56(9):1187-1195.  

[19] NTAMBI J M, SESSLER A M, TAKOVA T.A model cell line to study regulation of stearoyl-CoA desaturase gene 1 expression by insulin and polyunsaturated fatty acids[J].Biochemical and Biophysical Research Communications, 1996, 220(3):990-995.  

[20] LEFEVRE P, DIOT C, LEGRAND P, et al.Hormonal regulation of stearoyl coenzyme-A desaturase 1 activity and gene expression in primary cultures of chicken hepatocytes[J].Archives of Biochemistry and Biophysics, 1999, 368(2):329-337.  

[21] MAUVOISIN D, ROCQUE G, ARFA O, et al.Role of the PI3-kinase/mTOR pathway in the regulation of the stearoyl CoA desaturase (SCD1) gene expression by insulin in liver[J].Journal of Cell Communication and Signaling, 2007, 1(2):113-125.  

[22] HAN C, WANG J, ZHANG Z, et al.The role of insulin and glucose in goose primary hepatocyte triglyceride accumulation[J].Journal of Experimental Biology, 2009, 212(10):15553-15558.

[23] FERR P, FOUFEELE F.SREBP-1c transcription factor and lipid homeostasis:clinical perspective[J].Hormone Research, 2007, 68(2):72-82.

[24] DENTIN R, GIRARD J, POSTIC C.Carbohydrate responsive element binding protein (ChREBP) and sterol regulatory element binding protein-1c(SREBP-1c):two key regulators of glucose metabolism and lipid synthesis in liver[J].Biochimie, 2005, 87(1):81-86.  

[25] KOO S H, DUTCHER A K, TOWLE H C.Glucose and insulin function through two distinct transcription factors to stimulate expression of lipogenic enzyme genes in liver[J].Journal of Biological Chemistry, 2001, 276(12):9437-9445.  

[26] 韩春春, 王继文, 符自英, 等.葡萄糖和胰岛素对鹅肝细胞脂肪酸合成酶活性及转录表达的影响[J].农业生物技术学报, 2009, 17(4):634-637.
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