仔猪断奶前后肠道形态和相关免疫蛋白基因表达的变化

任曼; 霍应峰; 杨凤娟; 刘灵; 罗艳红; 谯仕彦

doi:10.3969/j.issn.1006-267x.2014.03.009

动物营养学报 >

2014 , Vol. 26 >Issue 3: 614 - 619

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.03.009

猪营养 Swine Nutrition

仔猪断奶前后肠道形态和相关免疫蛋白基因表达的变化

任曼 ,
霍应峰 ,
杨凤娟 ,
刘灵 ,
罗艳红 ,
谯仕彦

展开

1. 中国农业大学动物科技学院, 北京 100193;
2. 北京同力兴科农业科技有限公司, 北京 100193

收稿日期: 2013-09-05

网络出版日期: 2014-03-04

基金资助

国家重点基础研究发展计划（2012CB124704）

收起

The Changes of Intestinal Morphology and Immune-Related Protein Gene Expressions in Piglets before and after Weaning

REN Man ,
HUO Yingfeng ,
YANG Fengjuan ,
LIU Ling ,
LUO Yanhong ,
QIAO Shiyan

Expand

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. Beijing Tonglixingke Agricultural Science & Technology Co., Ltd., Beijing 100193, China

Received date: 2013-09-05

Online published: 2014-03-04

Fold

摘要

为了研究仔猪出生至断奶后肠道形态和相关免疫蛋白基因表达的变化，本试验于仔猪出生7、14和21 d以及断奶后14 d（出生35 d）分别从6窝中每窝挑选1头健康仔猪（共24头）屠宰获得十二指肠和空肠组织。利用苏木精-伊红染色检测小肠形态和淋巴细胞、杯状细胞计数，酶联免疫吸附法（ELISA）测定空肠组织中的分泌型免疫球蛋白A（sIgA）浓度，实时荧光定量PCR检测空肠组织中防御素基因的表达水平。结果表明：仔猪出生7~21 d，出生14和21 d的十二指肠和空肠的绒毛高度显著低于出生7 d（P<0.05），同时十二指肠的隐窝深度随着日龄的增加有逐渐降低的趋势，但肠上皮杯状细胞和淋巴细胞数目无显著变化（P>0.05）；断奶后14 d空肠绒毛高度较出生7 d显著降低（P<0.05），隐窝增生明显，隐窝深度较出生7~21 d显著增加（P<0.05），同时十二指肠和空肠上皮淋巴细胞数目均显著增多（P<0.05）。另外，较出生21 d，断奶后仔猪空肠分泌更多的sIgA（P=0.001）和表达更多的防御素基因（P<0.05）以保护肠黏膜。由此可知，断奶前，仔猪健康状况下肠道屏障完整，sIgA和防御素表达的水平相对较低，断奶破坏了肠道完整性，使得肠道绒毛萎缩，隐窝增生，肠道上皮屏障受到损伤，大量的淋巴细胞趋化至肠道上皮，同时断奶时微生物入侵刺激了肠上皮免疫球蛋白和防御素的分泌。

关键词： 仔猪; 断奶; 肠道形态; 分泌型免疫球蛋白A; 防御素

本文引用格式

任曼 , 霍应峰 , 杨凤娟 , 刘灵 , 罗艳红 , 谯仕彦 . 仔猪断奶前后肠道形态和相关免疫蛋白基因表达的变化[J]. 动物营养学报, 2014 , 26(3) : 614 -619 . DOI: 10.3969/j.issn.1006-267x.2014.03.009

Abstract

This study was conducted to investigate the changes of intestinal morphology and immune-related protein gene expressions in piglets before and after weaning. A total of 24 healthy piglets from 6 litters with 1 piglet per litter were slaughtered at 7, 14, 21 and 35 days of age (weaned at 21 days of age), then duodenum and jejunum were collected. The intestinal morphology, intra-epithelial lymphocytes number and goblet cells number, jejunal secretory immunoglobulin A (sIgA) concentration and defensin gene expressions were tested using haematoxylin and eosin staining, ELISA or real time quantitative PCR, respectively. The results showed as follows: during 7 d to 21 d after born, we found that the villous height of duodenum and jejunum was shorter in 14 and 21 d compared with 7 d (P<0.05), meanwhile the crypt depth in duodenum tended to be gradually decreased following age, but the intestinal epithelial goblet cells number and intra-epithelial lymphocytes number were not significantly changed (P>0.05). After weaning (35 d after born), in jejunum, the villous height was significantly decreased compared with 7 d (P<0.05) and the crypt depth was significantly increased compared with that before weaning (during 7 d to 21 d after born) (P<0.05). Compared with that before weaning, the intra-epithelial lymphocytes number of duodenum and jejunum was significantly increased after weaning (P<0.05). The sIgA concentration (P=0.001) and defensin gene expressions in jejunum of weaned piglets were significantly higher than those of before-weaning piglets (P<0.05). From the results, we can find that piglets have a healthy intestinal barrier and low-expression levels of sIgA and defensins before weaning. After weaning, the weaning stress disturbances the intestinal mucosal integrity because of the atrophy of the villi and the proliferation of crypt, and intestinal barrier is destroyed, meanwhile, the number of intra-epithelial lymphocytes is increased. The infection of bacteria stimulates the expressions of immunoglobulin and defensins after weaning.

Key words： piglet; weaning; intestinal morphology; sIgA; defensin

参考文献

[1] CUMMINS A G, THOMPSON F M.Effect of breast milk and weaning on epithelial growth of the small intestine in human[J].Gut, 2002, 51(5):748-754.

[2] MAO X, ZENG X, QIAO S, et al.Specific roles of threonine in intestinal mucosal integrity and barrier function[J].Frontiers in Bioscience, 2011, E3:1192-1200.

[3] 李德发.猪的营养[M].2版.北京:中国农业科学技术出版社, 2003:18-21.

[4] ROSE N, LAROUR G, DIGUERHER L G, et al.Risk factors for porcine post-weaning multisystemic wasting syndrome (PMWS) in 149 f French farrow-to finish herds[J].Prewentive Veterinary Medicine, 2003, 61(3):209-225.

[5] GALLO R L, HOOPER L V.Epithelial antimicrobial defence of the skin and intestine[J].Nature Reviews Immunology, 2012, 12(7):503-516.

[6] SANG Y, PATIL A A, ZHANG G, et al.Bioinformatic and expression analysis of novel porcine beta-defensins[J].Mammalian Genome, 2006, 17(4):332-339.

[7] SANTAOLALLA R, FUKATA M, ABREU T M.Innate immunity in the small intestine[J].Current Opinion in Gastroenterology, 2011, 27(2):125-131.

[8] 霍永久, 王恬, 许若军.新生仔猪小肠生长发育及肠粘膜部分基因的表达[J].南京农业大学学报, 2005, 28(3):129-132.

[9] CERA K R, MAHAN D C CROSS R F, et al.Effect of age, weaning and postweaning diet on small intestinal growth and jejunal morphology in young swine[J].Journal of Animal Science, 1988, 66(2):574-584.

[10] NABUURS M J A, HOOGENDOORN A, MOLEN VANDER E J, et al.Villus height and crypt depth in weaned and unweaned pigs, reared under various circumstances in the Netherlands[J].Research in Veterinary Science, 1993, 55(1):78-84.

[11] PLUSKE J R, THOMPSON M J, ATWOOD C S, et al.Maintenance of villus height and crypt depth, and enhancement of disaccharide digestion and monosaccharide absorption, in piglets fed on cows' whole milk after weaning[J].British Journal of Nutrition, 1996, 76(3):409-422.

[12] 范骏.肠道黏膜免疫[J].国际免疫学杂志, 2006, 29(2):111-115.

[13] NOFRARIAS M, MANZANILLA E G, PUJOLS J, et al.Effects of spray-dried porcine plasma and plant exreacts on intestinal morphology and on leukocyte cell subsets of weaned pigs[J].Journal of Animal Science, 2006, 84(10): 2735-2742.

[14] KINNEBREW M A, PAMER E G.Innate immune signaling in defense against intestinal microbes[J].Immunological Reviews, 2012, 245(1):113-131.

[15] 印遇龙.仔猪营养学[M].北京:中国农业出版社, 2010:35-41.

[16] ZHANG G, ROSS C R, BLECHA F.Porcine antimicrobial peptides:new peospects for ancient molecules of host defense[J].Veterinary Research, 2000, 31(3):277-296.

[17] MNARD S, FRSTER V, LOTZ M, et al.Developmental switch of intestinal antimicrobial peptide expression[J].Journal of Experimental Medicine, 2008, 205(1):183-193.

[18] CASH H L, WHITHAM C V, BEHRENDT C L, et al.Symbiotic bacteria direct expression of an intestinal bactericidal lectin[J].Science, 2006, 313:1126-1130.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们