禽营养 Poultry Nutrition

海兰褐壳蛋鸡含黄素单氧化酶3基因型频率分布及其对蛋品质的影响

  • 王晶 ,
  • 武书庚 ,
  • 张海军 ,
  • 岳洪源 ,
  • 齐广海
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  • 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放试验室, 北京 100081

收稿日期: 2013-10-12

  网络出版日期: 2014-03-04

基金资助

国家青年自然基金(31301991);国家蛋鸡产业技术体系(CARS-41-K13);家禽产业技术体系北京市创新团队项目(CARS-PSTP)

Flavin-Containing Monooxygenase 3 Genotype:Frequencies Distribution and Effects on Egg Quality in Hy-Line Brown Laying Hens

  • WANG Jing ,
  • WU Shugeng ,
  • ZHANG Haijun ,
  • YUE Hongyuan ,
  • QI Guanghai
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  • Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2013-10-12

  Online published: 2014-03-04

摘要

本试验旨在检测含黄素单氧化酶3(FMO3)T329S突变位点在我国商品代海兰褐壳蛋鸡群体中的分布,考察基因型对鸡蛋品质和鸡蛋中三甲胺(TMA)含量的影响。采用聚合酶链式反应-限制性片段长度多态性分析方法(PCR-RFLP)和DNA测序技术测定FMO3 T329S突变位点在商品代海兰褐壳蛋鸡群体中的分布。筛选已知FMO3基因型海兰褐壳蛋鸡120只,饲养6周,测定鸡蛋品质和鸡蛋中TMA含量。结果表明:1)商品代海兰褐壳产蛋鸡群体中,AA(野生型)、AT(杂合型)和TT(突变型)基因型频率分别为3.4%、76.6%和20.0%,极显著偏离了哈代-温伯格平衡(P<0.001)。2)基因型显著影响鸡蛋TMA含量,但对鸡蛋其他品质无显著影响(P>0.05);TT基因型蛋黄TMA含量显著升高(P<0.05),但低于嗅觉阈值。由此可见,海兰褐壳鸡群体中TT基因型出现频率较高(20.0%),其蛋中TMA含量较高;FMO3基因型不影响蛋品质。

本文引用格式

王晶 , 武书庚 , 张海军 , 岳洪源 , 齐广海 . 海兰褐壳蛋鸡含黄素单氧化酶3基因型频率分布及其对蛋品质的影响[J]. 动物营养学报, 2014 , 26(3) : 630 -636 . DOI: 10.3969/j.issn.1006-267x.2014.03.011

Abstract

This experiment was conducted to investigate the distribution of T329S mutation in flavin-containing monooxygenase 3 (FMO3) in Hy-Line brown laying hens, and the effects of FMO3 genotype on egg quality and egg trimethylamine (TMA) content. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) followed by DNA sequencing assay was developed to genotype laying hens. A total of 120 genotyped laying hens were selected and fed for 6 weeks, and the egg quality and egg TMA contents were analyzed. The results showed as follows: 1) the frequencies of AA (wild type), AT (heterozygous type) and TT (mutant type) genotype were 3.4%, 76.6% and 20.0%, respectively. The allelic frequencies were not in Hardy-Weinberg equilibrium in the Hy-Line brown laying hens strain (P<0.001). 2) Egg quality was not affected by the genotype (P>0.05) with the exception of TMA contents which was higher in TT hens (P<0.05). However, yolk TMA content remained below the human detection threshold for TT hens. The results indicate that the frequency of TT hens in Hy-Line brown strain is 20.0%, which show higher egg TMA content. Egg quality is not affected by the genotype.

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