研究简报 Short Communications

猪源唾液乳杆菌对生长猪生长性能、粪中微生物数量及血清指标的影响

  • 张董燕 ,
  • 季海峰 ,
  • 王四新 ,
  • 王晶 ,
  • 刘辉 ,
  • 王雅民
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  • 北京市农林科学院畜牧兽医研究所, 北京 100097

收稿日期: 2013-09-25

  网络出版日期: 2014-03-04

基金资助

生猪产业技术体系北京市创新团队建设项目(GWZJ-2009-06);北京市农林科学院青年科研基金(QNJJ201209)

Effects of L. salivarius from Pigs on Growth Performance, the Number of Faecal Microflora and Serum Indices of Growing Pigs

  • ZHANG Dongyan ,
  • JI Haifeng ,
  • WANG Sixin ,
  • WANG Jing ,
  • LIU Hui ,
  • WANG Yamin
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  • Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received date: 2013-09-25

  Online published: 2014-03-04

摘要

本试验旨在探讨猪源唾液乳杆菌(L. salivarius)对生长猪生长性能、粪中微生物数量及血清指标的影响。选用平均体重为(35.22±0.86)kg的长×大二元杂交生长猪81头,随机分成3组,每组3个重复,每个重复9头。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加0.2%、0.4%(每克饲粮活菌数分别为7.0×106和1.4×107 CFU)的猪源L. salivarius冻干制剂。试验期为30 d。结果表明:1)与对照组相比,试验组生长猪的平均日采食量(ADFI)和料重比(F/G)无显著变化(P>0.05),平均日增重(ADG)有升高趋势(P>0.05);2)饲粮中添加0.2%、0.4%的猪源L. salivarius能够显著增加生长猪粪中乳酸菌数量(P<0.05),显著降低大肠杆菌数量(P<0.05),并显著提高乳酸菌与大肠杆菌的比值(P<0.05);3)与对照组相比,试验组生长猪血清总胆固醇(TCHO)含量及谷丙转氨酶(ALT)、谷草转氨酶(AST)活性显著降低(P<0.05);4)试验组生长猪血清结合珠蛋白(Hp)含量均较对照组显著降低(P<0.05)。结果提示,饲粮中添加猪源L. salivarius能够改善生长猪肠道菌群环境和血清指标,提高机体免疫力,进而促进其健康生长。

本文引用格式

张董燕 , 季海峰 , 王四新 , 王晶 , 刘辉 , 王雅民 . 猪源唾液乳杆菌对生长猪生长性能、粪中微生物数量及血清指标的影响[J]. 动物营养学报, 2014 , 26(3) : 725 -731 . DOI: 10.3969/j.issn.1006-267x.2014.03.023

Abstract

This experiment was conducted to study the effects of L. salivarius from pigs on growth performance, the number of faecal microflora and serum indices of growing pigs. Eighty-one crossbred (Landrace×Large White) growing pigs with an average body weight of (35.22±0.86) kg were divided into 3 groups with 3 replicates per group and 9 pigs per replicate. Pigs in control group were fed a basal diet and those in experimental groups were fed experimental diets supplemented with 0.2% and 0.4% (viable bacteria counts were 7.0×106 and 1.4×107 CFU per gram of diets, respectively) lyophilized preparation of L. salivarius from pigs, respectively. The experiment lasted for 30 days. The results showed as follows: 1) compared with the control group, the average daily feed intake (ADFI) and feed/gain (F/G) of growing pigs in the experimental groups had no significant changes (P>0.05), and the average daily gain (ADG) had increasing trends (P>0.05); 2) the supplementation of L. salivarius at 0.2% and 0.4% could significantly increase the number of Lactobacilli (P<0.05), significantly reduce the number of E. coli (P<0.05), and significantly increase the ratio of Lactobacilli to E. coli in the faeces (P<0.05); 3) compared with the control group, the activities of alanine transferase (ALT) and aspartate transferase (AST) and the content of total cholesterol (TCHO) in serum in the experimental groups were significantly decreased (P<0.05); 4) compared with the control group, the content of haptoglobin (Hp) in serum in the experimental groups was significantly decreased (P<0.05). The results indicate that the supplementation of L. salivarius from pigs can improve the environment of intestinal microflora and the indices of serum, increase the immunity of body, and then advance the growth of growing pigs.

参考文献

[1] CHANG C Y, CHEN Y T, CHUEN F L, et al.Characterization of tetracycline restance Lactobcilli isolated from swine intestines at weatern area of Taiwan[J].Anaerobe, 2011, 17(5):239-245.  

[2] COMUNIAN R, DAGA E, DUPRE I, et al.Susceptibility to tetracycline and erythromycin of Lactobacillus paracasei strains isolated from traditional Italian fermented foods[J].International Journal of Food Microbiol, 2010, 138(1/2):151-156.

[3] TIMMERMAN H M, VELDMAN A, VAN DEN ELSEN E, et al.Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics[J].Poultry Science, 2006, 85(8):1383-1388.  

[4] GARDINER G E, CASEY P G, CASEY G, et al.Relative ability of orally administered Lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract[J].Applied and Environmental Microbiology, 2004, 70(4):1895-1906.  

[5] ZOETENDAL E G, COLLIER C T, KOIKE S, et al.Molecular ecological analysis of the gastrointestinal microbiota:a review[J].The Journal of Nutrition, 2004, 134(2):465-472.

[6] DAVIS M E, BROWN D C, BAKER A, et al.Effect of direct-fed microbial and antibiotic supplementation on gastrointestinal microflora, mucin histochemical characterization, and immune populations of weanling pigs[J].Livestock Science, 2007, 108(1/2/3):249-253.

[7] BROOM L J, MILLER H M, KERR K G, et al.Effects of zinc oxide and Enterococcus faecium SF68 dietary supplementation on the performance, intestinal microbiota and immune status of weaned piglets[J].Research in Veterinary Science, 2006, 80(1):45-54.  

[8] HUANG C, QIAO S, LI D, et al.Effects of Lactobacillus on the performance, diarrhoea incidence, VFA concentration and gastrointestinal microbial flora of weaning pigs[J].Asian-Austral Journal of Animal Sciences, 2004, 17(3):401-409.

[9] PLUSKE J R.Morphological and functional changes in the small intestine of the newly-weaned pig[M]//PIVA A B, KNUDSEN K E, LINDBERG J E.Gut Environment of Pigs.Nottingham:Nottingham University Press, 2001:1-27.

[10] ESTIENNE M J, HARTSOCK T G, HARPER A F.Effects of antibiotics and probiotics on suckling pig and weaned pig performance[J].International Journal of Applied Research in Veterinary Medicine, 2005, 3:303-308.

[11] GIANG H H, VIET T Q, OGLE B, et al.Growth performance, digestibility, gut environment and health status in weaned pigs fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science, 2010, 129(1):95-103.

[12] NOUSIAINEN J, JAVANINEN P, SETALA J.Lactic acid bacteria microbiological and functional aspects[M]//SALMINEN S, VON WRIGHT A, OUWEHAND A.Lactic acid bacteria as animal probiotics.New York:Marcel Dekker Inc., 2004:547-580.

[13] LEE Y K, LIM C Y, TENG W L, et al.Quantitative approach in the study of adhesion of lactic acid bacteria to intestinal cells and their competition with enterobacteria[J].Applied and Environmental Microbiology, 2000, 66(9):3692-3697.  

[14] CASTILLO M S, MARTÍN-ORÙE M, MANZANILLA E G, et al.Quantification of total bacteria, Enterobacteria and Lactobacilli populations in pig digesta by real-time PCR[J].Veterinary Microbiology, 2006, 114(1/2):165-170.

[15] MURALIDHARA K S, SHEGGEBY G G, ELLIKER P R, et al.Effect of feeding Lactobacilli on the coliform and Lactobacillus flora of intestinal tissue and feces from piglets[J].Journal of Food Protection, 1977, 40:288-293.

[16] CASTILLO M, MARTÍN-ORÙE S M, ANGUITA M, et al.Adaptation of gut microbiota to corn physical structure and different types of dietary fibre[J].Livestock Science, 2007, 109(1/2/3):149-152.

[17] 侯璐.猪源粪肠球菌的特性及对仔猪生长性能和免疫力影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学, 2010:40-41.

[18] MALLO J J, RIOPEREZ J, HONRUBIA P.The addition of Enterococcus faecium to diet improves piglet's intestinal microbiota and performance[J].Livestock Science, 2010, 133(1):176-178.

[19] 侯成立.猪源益生乳杆菌的分离、筛选及应用研究[D].硕士学位论文.呼和浩特:内蒙古农业大学, 2012:49-51.

[20] ZHU J.A review of microbiology in swine manure odor control[J].Agriculture Ecosystems and Environment, 2000, 78(2):93-106.  

[21] BONGAERTS G, SEVERIJNEN R, TIMMERMAN H.Effect of antibiotics, prebiotics and probiotics in treatment for hepatic encephalopathy[J].Medical Hypotheses, 2005, 64(1):64-68.  

[22] LYE H S, RUSUL G, LIONG M T.Removal of cholesterol by Lactobacilli via incorporation and conversion to coprostanol[J].Journal of Dairy Science, 2010, 93(4):1383-1392.  

[23] PINEIRO M, GYMNICH S, KNURA S, et al.Meat juice:an alternative matrix for assessing animal health by measuring acute phase proteins.Correlations of pig-MAP and haptoglobin concentrations in pig meat juice and plasma[J].Research in Veterinary Science, 2009, 87(2):273-276.  

[24] WITTEN I.Ways of evaluating the health status of slaughter pigs and its effect on the storage life of pork by using haptoglobin analysis[D].Ph.D.Thesis.Hannover:University of Veterinary Medicine, 2005:63-65.

[25] LE F N, JONDREVILLE C, MATTE J J, et al.Importance of sanitary environment for growth performance and plasma nutrient homeostasis during the post-weaning period in pigs[J].Archives of Animal Nutrition, 2006, 60(1):23-34.  

[26] PETERSEN H H, NIELSEN J P, HEEGAARD P M.Application of acute phase protein measurements in veterinary clinical chemistry[J].Veterinarian Research, 2004, 35(2):163-187.

[27] 许美芳.血液急性期蛋白作为猪群健康评价指标的研究[D].硕士学位论文.金华:浙江师范大学, 2011:35-37.
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