动物营养学报 >

2014 , Vol. 26 >Issue 3: 819 - 826

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.03.035

研究简报 Short Communications

解淀粉芽孢杆菌SC06对免疫抑制小鼠肠道细菌酶活性和肠黏膜屏障功能的影响

  • 杜威 ,
  • 黄琴 ,
  • 付爱坤 ,
  • 余东游 ,
  • 李卫芬
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  • 浙江大学动物科学学院饲料科学研究所, 农业部动物营养与饲料重点开放实验室, 杭州 310029

收稿日期: 2013-09-17

  网络出版日期: 2014-03-04

基金资助

国家863计划项目（2013AA102800）；“十二五”农村领域国家科技计划课题（2013BAD10B02-03）

收起

Effects of Bacillus amyloliquefaciens SC06 on Intestinal Bacterial Enzyme Activities and Intestinal Mucosal Barrier Function of Immunosuppressive Mice

  • DU Wei ,
  • HUANG Qin ,
  • FU Aikun ,
  • YU Dongyou ,
  • LI Weifen
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  • Key Laboratory of Animal Nutrition and Feed Science of Ministry of Agriculture, Institute of Feed Science, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China

Received date: 2013-09-17

  Online published: 2014-03-04

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摘要

本试验通过连续3 d腹腔注射免疫抑制剂环磷酰胺（cyclophosphamide，CTX）建立小鼠免疫抑制模型，研究解淀粉芽孢杆菌SC06对免疫抑制小鼠肠道细菌酶活性和肠黏膜屏障功能的影响。选取120只BALB/c小鼠（4周龄，雌性，体重10~16 g），随机分成3组，每组4个重复，每个重复10只。对照组饲喂基础饲粮，免疫抑制组和芽孢杆菌组分别饲喂基础饲粮和添加解淀粉芽孢杆菌SC06的试验饲粮（活菌数为1×105 CFU/g），并均在试验第1~3天连续3 d腹腔注射CTX 80 μg/g BW。试验期为60 d。结果表明：与对照组相比，免疫抑制组小鼠盲肠和小肠内容物中各细菌酶（α-葡萄糖苷酶、β-葡萄糖苷酶、β-葡萄糖苷酸酶、α-半乳糖苷酶和β-半乳糖苷酶）以及小肠灌洗液中二胺氧化物酶（DAO）活性极显著降低（P<0.01），小肠灌洗液中一氧化氮（NO）含量及诱导型一氧化氮合成酶（iNOS）活性极显著升高（P<0.01），小肠灌洗液中促炎细胞因子增殖诱导配体（APRIL）、干扰素-γ（IFN-γ）、白细胞介素-6（IL-6）和白细胞介素-12（IL-12）及抗炎细胞因子白细胞介素-10（IL-10）含量极显著升高（P<0.01），说明腹腔注射CTX后小鼠肠道菌群发生紊乱，肠上皮细胞遭到破坏，肠黏膜屏障功能受损。解淀粉芽孢杆菌SC06可极显著提高免疫抑制小鼠盲肠内容物中β-葡萄糖苷酸酶和α-葡萄糖苷酶及小肠内容物中β-葡萄糖苷酸酶活性（P<0.01），分别极显著和显著提高小肠灌洗液中髓过氧化物酶（MPO）和分泌型磷脂酶A2（sPLA2）活性（P<0.01和P<0.05）。以上结果表明解淀粉芽孢杆菌SC06可在一定程度上提高免疫抑制小鼠的肠道益生菌数量，但仅能部分恢复免疫抑制小鼠的肠黏膜屏障功能。

关键词： 解淀粉芽孢杆菌SC06; 免疫抑制; 小鼠; 细菌酶活性; 肠黏膜屏障功能

本文引用格式

杜威 , 黄琴 , 付爱坤 , 余东游 , 李卫芬 . 解淀粉芽孢杆菌SC06对免疫抑制小鼠肠道细菌酶活性和肠黏膜屏障功能的影响[J]. 动物营养学报, 2014 , 26(3) : 819 -826 . DOI: 10.3969/j.issn.1006-267x.2014.03.035

Abstract

This study was conducted to study the effects of Bacillus amyloliquefaciens SC06 on intestinal bacterial enzyme activities and intestinal mucosal barrier function of immunosuppressive mice. The model of immunosuppressive mice was built by intraperitoneal injection of cyclophosphamide (CTX) for 3 days continuously. A total of 120 BALB/c mice (4-week-old, female and body weight of 10 to 16 g) were randomly divided into 3 groups with 4 replicates per group and 10 mice per replicate. The mice in control group and immunosuppressive group were all fed basal diet, and those in Bacillus group were fed the basal diet supplemented with Bacillus amyloliquefaciens SC06 (living bacteria count was 1×105 CFU/g). On the 1st to 3rd day of the experiment, the mice in immunosuppressive group and Bacillus group were intraperitoneally injected with 80 μg/g BW of CTX for 3 consecutive days. The experiment lasted for 60 days. The results showed as follows: compared with the control group, the activities of bacterial enzymes (including α-glucosidase, β-glucosidase, β-glucuronidase, α-galactosidase and β-galactosidase) in cecal and small intestinal contents and diamine oxidase (DAO) in small intestinal lavage fluid were significantly decreased (P<0.01), the content of nitric oxide (NO) and the activity of inducible nitric oxide synthase (iNOS) in small intestinal lavage fluid were significantly increased (P<0.01), and the contents of pro-inflammatory cytokines—aproliferation inducingligand (APRIL), interferon-γ (IFN-γ), interleukin-6 (IL-6) and interleukin-12 (IL-12) and anti-inflammatory cytokine—interleukin-10 (IL-10) in small intestinal lavage fluid were significantly increased (P<0.01). It indicated that the intestinal flora of mice was disordered, and the intestinal mucosa epithelial cells and the intestinal mucosal barrier function were damaged by intraperitoneal injection of CTX. Bacillus amyloliquefaciens SC06 could significantly increase the activities of β-glucuronidase and α-glucosidase in cecal contents and β-glucuronidase in small intestinal contents (P<0.01), and significantly increase the activities of myeloperoxidase (MPO) and secretory phospholipase A2 (sPLA2) in small intestinal lavage fluid (P<0.01 and P<0.05, respectively). The results indicate that Bacillus amyloliquefaciens SC06 can increase the intestinal probiotic count in a certain extent, but only can partially recover the intestinal mucosal barrier function.

Key words： Bacillus amyloliquefaciens SC06; immunosuppression; mice; bacterial enzyme activities; intestinal mucosal barrier function

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