富硒益生菌对罗非鱼幼鱼生长和抗氧化水平的影响

柯浩; 刘振兴; 曹艳林; 马艳平; 郝乐; 马江耀; 梁志凌

doi:10.3969/j.issn.1006-267x.2014.05.013

动物营养学报 >

2014 , Vol. 26 >Issue 5: 1229 - 1237

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.05.013

水产营养 Aquaculture Nutrition

富硒益生菌对罗非鱼幼鱼生长和抗氧化水平的影响

柯浩 ,
刘振兴 ,
曹艳林 ,
马艳平 ,
郝乐 ,
马江耀 ,
梁志凌

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广东省农业科学院动物卫生研究所, 广东省兽医公共卫生公共实验室, 广州 510640

收稿日期: 2013-11-11

网络出版日期: 2014-05-05

基金资助

广州市农科科技攻关项目（GZCQC1202FG04002-01）；广东省海洋渔业科技推广专项（A201001D05，A201201C01）；广州市科技计划项目（201300000064）

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Effects of Selenium-Enriched Probiotics on Growth and Antioxidant Level of Juvenile Tilapia (Oreochromis niloticus)

KE Hao ,
LIU Zhenxing ,
CAO Yanlin ,
MA Yanping ,
HAO Le ,
MA Jiangyao ,
LIANG Zhiling

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Guangdong Common Lab of Veterinary Public Health, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2013-11-11

Online published: 2014-05-05

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摘要

本试验旨在研究富硒益生菌对罗非鱼幼鱼生长及抗氧化水平的影响。试验选取600尾罗非鱼幼鱼，随机分为5组，每组设3个重复，每个重复随机放养初始体重为（12.40±0.13） g的罗非鱼幼鱼40尾。其中，对照组饲喂硒含量为0.257 mg/kg的基础饲料，其他4组分别饲喂在基础饲料中添加由富硒酵母菌、酵母菌、乳酸菌组成的富硒益生菌的试验饲料。4种试验饲料中富硒酵母菌的添加量折算为硒添加量分别为0（A组）、0.4（B组）、0.6（C组）、0.8 mg/kg（D组），乳酸菌的添加量均为3.04×10⁵ CFU/g，并通过调节酵母菌添加量使4种试验饲料中添加的总活菌数相同。试验期为70 d。结果表明：D组和C组分别在第50~60天和第60~70天阶段表现出最高的特定生长率，并均显著高于对照组（P<0.05）。C组血清谷胱甘肽过氧化物酶（GPx）活性在第30天和第50天均显著高于A组（P<0.05）；对照组血清GPx活性在第60天显著低于其他各组（P<0.05）。在第30天，血清过氧化氢酶（CAT）活性以C组最高、A组最低，各组间均差异显著（P<0.05）；对照组血清CAT活性在第60天显著低于A组、B组和C组（P<0.05）。C组血清超氧化物歧化酶（SOD）活性在第30天和第50天显著高于其他各组（P<0.05）。各组罗非鱼的肝脏谷胱甘肽过氧化物酶1（GPx1）mRNA相对表达量均随饲养天数的延长呈现出上升态势，至第50天达到最高值后开始呈下降的趋势。在第50天，A组和C组的肝脏GPx1 mRNA的相对表达量显著高于其他各组（P<0.05），且以C组的值最高。由上述结果可知，在含硒0.257 mg/kg的基础饲料中添加0.6 mg/kg的富硒益生菌（以硒计）可促进罗非鱼幼鱼（体重12~55 g/尾）的生长，提高其抗氧化水平。

关键词： 富硒益生菌; 罗非鱼; 生长; GPx1 mRNA相对表达量; 抗氧化水平

本文引用格式

柯浩 , 刘振兴 , 曹艳林 , 马艳平 , 郝乐 , 马江耀 , 梁志凌 . 富硒益生菌对罗非鱼幼鱼生长和抗氧化水平的影响[J]. 动物营养学报, 2014 , 26(5) : 1229 -1237 . DOI: 10.3969/j.issn.1006-267x.2014.05.013

Abstract

This experiment was conducted to study the effects of selenium-enriched probiotics on growth and antioxidant level of juvenile tilapia (Oreochromis niloticus). Six hundred juvenile tilapia with an average initial body weight of (12.40±0.13) g were randomly chosen and distributed into 5 groups with 3 replicates in each group and 40 fish in each replicate. The fish in control group were fed a basal diet containing 0.257 mg/kg selenium, and the fish in other four groups were fed experimental diets supplemented with selenium-enriched probiotics. The selenium-enriched probiotics were composed by selenium-enriched yeast, yeast and lactic acid bacteria with different proportions. The supplemental levels of selenium-enriched yeast converted into selenium supplemental levels were 0 (group A), 0.4 (group B), 0.6 (group C) and 0.8 mg/kg (group D), respectively, while the supplemental levels of lactic acid bacteria were all 3.04×10⁵ CFU/g, and the supplemental levels of living bacteria were the same by adjusting yeast supplemental level in the 4 experimental diets. The experiment lasted for 70 days. The results showed as follows: the highest of special growth rate in the stages of days 50 to 60 and days 60 to 70 was found in groups D and C, respectively, and was significantly higher than that in control group (P<0.05). The activity of serum glutathione peroxidase (GPx) on day 30 and day 50 in group C was significantly higher than that in group A (P<0.05), and the activity of serum GPx on day 60 in control group was significantly lower than that in other groups (P<0.05). On day 30, groups C and A had the highest and the lowest serum catalase (CAT) activity, respectively, and there was significant difference in serum CAT activity among all groups (P<0.05). The activity of serum CAT on day 60 in control group was significantly lower than that in groups A, B and C (P<0.05). The activity of serum superoxide dismutase (SOD) on day 30 and day 50 in group C was significantly higher than that in other groups (P<0.05). The relative expression level of liver glutathione peroxidase 1 (GPx1) mRNA in each group was firstly increased with feeding time extending; subsequently, it was decreased after reached the highest value on day 50. The relative expression level of liver GPx1 mRNA in groups A and C was significantly higher than that in other groups (P<0.05), and group C had the highest value. In conclusion, the supplementation of 0.6 mg/kg selenium-enriched probiotics (counted with selenium) in the basal diet with 0.257 mg/kg selenium can improve the growth and antioxidant level of juvenile tilapia with the body weight of 12 to 55 g per fish.

Key words： selenium-enriched probiotics; tilapia (Oreochromis niloticus); growth; GPx1 mRNA relative expression level; antioxidant level

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