饲料中添加&alpha;-酮戊二酸对草鱼热休克蛋白70和过氧化物酶体增殖物激活受体&gamma;mRNA表达的影响

梁健; 赵玉蓉; 王红权; 金柏涛; 唐德约; 伍琴

doi:10.3969/j.issn.1006-267x.2014.05.032

动物营养学报 >

2014 , Vol. 26 >Issue 5: 1379 - 1386

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.05.032

研究简报 Short Communications

饲料中添加α-酮戊二酸对草鱼热休克蛋白70和过氧化物酶体增殖物激活受体γmRNA表达的影响

梁健 ,
赵玉蓉 ,
王红权 ,
金柏涛 ,
唐德约 ,
伍琴

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湖南农业大学动物科学技术学院, 长沙 410128

收稿日期: 2013-11-14

网络出版日期: 2014-05-05

基金资助

湖南省自然科学基金（14JJ4039）；湖南省发改委试验项目（[2013]766）

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Effects of Dietary α-Ketoglutarate on Heat Shock Protein 70 and Peroxisome Proliferator Activated Receptor γ mRNA Expressions of Grass Carp (Ctenopharynodon idellus)

LIANG Jian ,
ZHAO Yurong ,
WANG Hongquan ,
JIN Baitao ,
TANG Deyue ,
WU Qin

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College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China

Received date: 2013-11-14

Online published: 2014-05-05

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摘要

本试验旨在探讨饲料中添加α-酮戊二酸（AKG）对草鱼热休克蛋白70（HSP70）和过氧化物酶体增殖物激活受体γ（PPARγ）mRNA表达的影响。选取平均体重为（35.66±0.39） g的健康草鱼450尾，随机分为5组（每组3个重复，每个重复30尾鱼）：对照组饲喂基础饲料，试验组分别饲喂以0.25%、0.50%、0.75%、1.00% AKG等量替换基础饲料中次粉的试验饲料。试验期为60 d，试验结束后采用实时荧光定量PCR方法检测草鱼肝胰脏、鳃、肾脏及脾脏中HSP70和PPARγ mRNA的相对表达量。结果表明：HSP70和PPARγ mRNA的相对表达量在不同组织中存在差异，二者均在肾脏中高度表达，HSP70 mRNA的相对表达量在肾脏、肝胰脏、脾脏、鳃中依次降低，而PPARγ mRNA的相对表达量在肾脏、肝胰脏、鳃、脾脏中依次降低。饲料中添加AKG显著或极显著影响HSP70和PPARγ mRNA在鳃、肾脏、脾脏中的表达以及PPARγ mRNA在肝胰脏中的表达（P<0.05或P<0.01）。与对照组相比，1.00% AKG组鳃中HSP70、PPARγ mRNA的相对表达量显著或极显著增加（P<0.05或P<0.01），0.75% AKG组脾脏中HSP70、PPARγ mRNA的相对表达量显著或极显著增加（P<0.05或P<0.01），各AKG添加组肾脏中HSP70、PPARγ mRNA的相对表达量均显著降低（P<0.05）；0.25% AKG组肝胰脏中PPARγ mRNA的相对表达量极显著高于其他各组（P<0.01），但肝胰脏中HSP70 mRNA的相对表达量在各组间差异不显著（P>0.05）。综合以上结果，建议草鱼（体重在35 g左右）饲料中AKG的添加量为0.75%。

关键词： 草鱼; α-酮戊二酸; 热休克蛋白70; 过氧化物酶体增殖物激活受体γ; 表达

本文引用格式

梁健 , 赵玉蓉 , 王红权 , 金柏涛 , 唐德约 , 伍琴 . 饲料中添加α-酮戊二酸对草鱼热休克蛋白70和过氧化物酶体增殖物激活受体γmRNA表达的影响[J]. 动物营养学报, 2014 , 26(5) : 1379 -1386 . DOI: 10.3969/j.issn.1006-267x.2014.05.032

Abstract

This experiment was conducted to investigate the effects of α-ketoglutarate (AKG) on heat shock protein 70 (HSP70) and peroxisome proliferator activated receptor γ (PPARγ) mRNA expressions of grass carp (Ctenopharynodon idellus). A total of 450 grass carp with an average body weight of (35.66±0.39) g were randomly allotted to 5 groups with 3 replicates per group and 30 fish per replicate. Fish in control group were fed a basal diet, while those in experimental groups were fed experiemental diets supplemented with 0.25%, 0.50%, 0.75% and 1.00% AKG to substitute equivalent wheat middling on the basis of basal diet, respecitively. The experiment lasted for 60 days. An real-time quantitative PCR method was used to measure the relative expression levels of HSP70 and PPARγ mRNA in hepatopancreas, gill, kidney and spleen of grass carp at the end of experiment. The results showed as follows: there were differences in the relative expression levels of HSP70 and PPARγ mRNA among different tissues, and both of them were highly expressed in kindy. The relative expression level of HSP70 mRNA in kindy, hepatopancreas, spleen and gill was systematically decreased, while the relative expression level of PPARγ mRNA in kindy, hepatopancreas, gill and spleen was systematically decreased. AKG supplementation significantly affected the expressions of HSP70 and PPARγ mRNA in gill, kindy and spleen and the expression of HSP70 mRNA in hepatopancreas (P<0.05 or P<0.01). Compared with the control group, the relative expression levels of HSP70 and PPARγ mRNA in gill were significantly increased in the 1.00% AKG group (P<0.05 or P<0.01), and the relative expression levels of HSP70 and PPARγ mRNA in spleen were significantly increased in the 0.75% AKG group (P<0.05 or P<0.01), while the relative expression levels of HSP70 and PPARγ mRNA in kindy were significantly decreased in all AKG supplemental groups (P<0.05). The relative expression level of PPARγ mRNA in hepatopancreas in 0.25% AKG group was significantly higher than that in other groups (P<0.01), however, the relative expression level of HSP70 mRNA in hepatopancreas showed no significant difference among all groups (P>0.05). Based on above results, the recommended supplemental level of AKG in diets of grass carp (the body weight is about 35 g) is 0.75%.

Key words： grass carp; AKG; HSP70; PPARγ; expression

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