动物营养学报 >

2014 , Vol. 26 >Issue 7: 1760 - 1768

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.07.007

禽营养 Poultry Nutrition

维生素D3对1~4周龄青农灰鹅血清生化指标、免疫性能及维生素D受体mRNA表达量的影响

  • 王宝维 ,
  • 王迪 ,
  • 葛文华 ,
  • 张名爱 ,
  • 贾玉辉 ,
  • 程凡升
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  • 1. 青岛农业大学优质水禽研究所, 青岛 266109;
    2. 青岛农业大学食品科学与工程学院, 青岛 266109

收稿日期: 2014-01-01

  网络出版日期: 2014-07-01

基金资助

国家水禽产业技术体系专项基金（CARS-43-11）；青岛市科技支撑计划项目（12-1-3-17-nsh）

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Effects of Vitamin D3 on Serum Biochemical Parameters, Immune Function and Vitamin D Receptor mRNA Expression Level of Qingnonghui Geese Aged from 1 to 4 Weeks

  • WANG Baowei ,
  • WANG Di ,
  • GE Wenhua ,
  • ZHANG Ming ,
  • JIA Yuhui ,
  • CHENG Fansheng
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  • 1. Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China;
    2. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2014-01-01

  Online published: 2014-07-01

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摘要

本试验旨在研究维生素D3对1~4周龄青农灰鹅血清生化指标、免疫性能及维生素D受体（VDR）mRNA表达量的影响，以确定鹅饲粮中维生素D3的适宜添加水平。选择1日龄青农灰鹅360只，随机分为6个组，每组6个重复，每个重复10只鹅。Ⅰ组为对照组，饲喂玉米-豆粕型基础饲粮；Ⅱ~Ⅵ组分别在基础饲粮中添加200、400、800、1 600、3 200 IU/kg维生素D3。试验期为4周。结果表明：1）Ⅴ、Ⅵ组血清钙、磷、维生素D3含量显著高于Ⅰ组（P<0.05），Ⅱ~Ⅵ组血清碱性磷酸酶活性显著低于Ⅰ组（P<0.05），各组血清钙结合蛋白、骨钙素、降钙素、甲状旁腺素含量无显著差异（P>0.05）。2）Ⅲ组胸腺指数显著高于其他各组（P<0.05），各组脾脏指数、法氏囊指数及血清溶菌酶活性无显著差异（P>0.05）。3）Ⅳ组免疫前和免疫后7 d禽流感抗体效价显著高于Ⅰ组（P<0.05），VDR mRNA表达量显著或极显著高于其他各组（P<0.05或P<0.01）。由此可见，饲粮添加200~800 IU/kg维生素D3能提高1~4周龄鹅血清生化指标、免疫性能和VDR mRNA表达量。

关键词： 维生素D3; 鹅; 血清生化指标; 免疫性能; mRNA表达量

本文引用格式

王宝维 , 王迪 , 葛文华 , 张名爱 , 贾玉辉 , 程凡升 . 维生素D3对1~4周龄青农灰鹅血清生化指标、免疫性能及维生素D受体mRNA表达量的影响[J]. 动物营养学报, 2014 , 26(7) : 1760 -1768 . DOI: 10.3969/j.issn.1006-267x.2014.07.007

Abstract

This experiment was conducted to study the effects of vitamin D3 on serum biochemical parameters, immune function and vitamin D receptor (VDR) mRNA expression level of Qingnonghui geese aged from 1 to 4 weeks, and to find the appropriate supplemental level of vitamin D3 in diets of geese. A total of 360 Qingnonghui geese (one-day-old) were randomly divided into 6 groups with 6 replicates per group and 10 geese per replicate. Geese in group Ⅰ (control group) were fed a corn-soybean meal basal diet, and geese in groups Ⅱ to Ⅵ were fed the basal diets supplemented with 200, 400, 800, 1 600 and 3 200 IU/kg vitamin D3, respectively. The experiment lasted for 4 weeks. The results showed as follows: 1) the contents of calcium, phosphorus and vitamin D3 in serum in groups Ⅴ and Ⅵ were significantly higher than those in group Ⅰ (P<0.05), while there were no significant effects on the contents of calcium binding protein, bone gla protein, calcitonin and parathormone in serum among all groups (P>0.05). 2) The hymus index in group Ⅲ was significantly higher than that in other groups (P<0.05), while there were no significant effects on the spleen index, bursa of Fabricius index and serum lysozyme activity among all groups (P>0.05). 3) The bird flu antibody titer at pre-immune and immune after 7 days in group Ⅳ were significantly higher than that in group Ⅰ (P<0.05), and the VDR mRNA expression level in group Ⅳ were significantly higher than that in other groups (P<0.05 or P<0.01). In conclusion, dietary supplemented with 200 to 800 IU/kg vitamin D3 can increase the serum biochemical parameters, immune function and VDR mRNA expression level of geese aged from 1 to 4 weeks.

Key words： vitamin D3; geese; serum biochemical parameter; immune function; mRNA expression level

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