动物营养学报 >

2014 , Vol. 26 >Issue 7: 1822 - 1829

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.07.014

反刍动物营养 Ruminant Nutrition

钼对镉胁迫下山羊血清抗氧化指标及肝脏凋亡相关基因表达的影响

  • 庄煜 ,
  • 王琦 ,
  • 曹华斌 ,
  • 胡国良 ,
  • 罗军荣 ,
  • 顾小龙
展开
  • 江西农业大学动物科学技术学院, 动物群发性疾病监测和防治研究所, 南昌 330045

收稿日期: 2014-02-25

  网络出版日期: 2014-07-01

基金资助

国家自然科学基金资助项目（31101863）；江西省自然科学基金（2010GQN0052）；江西省教育厅科学基金资助项目（GJJ10103）

收起

Effects of Molybdenum on Serum Anti-Oxidative Indices and Hepatic Apoptosis Gene Expressions of Dairy Goats Challenged by Cadmium

  • ZHUANG Yu ,
  • WANG Qi ,
  • CAO Huabin ,
  • HU Guoliang ,
  • LUO Junrong ,
  • GU Xiaolong
Expand
  • Institution for the Surveillance and Control of Animal Population Disease, College of Animal Science and Technology, Jiangxi Agriculture University, Nanchang 330045, China

Received date: 2014-02-25

  Online published: 2014-07-01

Fold

摘要

本试验旨在研究钼对镉胁迫下山羊血清抗氧化指标及肝脏凋亡相关基因表达的影响。选用36头健康波尔山羊随机分成4组，每组9头，试验Ⅱ组、试验Ⅲ组和试验Ⅳ组灌服1 mg/kg BW镉，再分别灌服15、30和45 mg/kg BW钼，试验Ⅰ组口服其他3组平均剂量的去离子水。试验期50 d，于第0、10、20、30、40、50天采血测定血清总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、一氧化氮（NO）含量、一氧化氮合酶（NOS）活性，于第0、25、50天屠宰取肝组织测定B淋巴细胞瘤-2相关X蛋白（Bax）和B淋巴细胞瘤-2（Bcl-2）基因表达量。结果表明：1）与试验Ⅰ组比较，试验Ⅱ组、试验Ⅲ组和试验Ⅳ组均能够显著降低血清T-AOC、SOD活性及肝脏Bcl-2基因表达量（P<0.05），显著升高血清NOS活性、MDA含量、NO含量及肝脏Bax基因表达量（P<0.05）；且随着试验天数的增加，血清T-AOC、SOD活性及肝脏Bcl-2基因表达量出现显著下降（P<0.05），血清MDA含量、NO含量、NOS活性及肝脏Bax基因表达量出现显著上升（P<0.05）。2）试验Ⅱ组、试验Ⅲ组和试验Ⅳ组间比较，当镉灌服量一定时，随着钼灌服量的升高，血清T-AOC、SOD活性呈下降趋势，血清MDA含量、NOS活性及肝脏Bax基因表达量呈上升趋势。结果提示，钼、镉可导致山羊血清抗氧化能力的下降同时诱导肝脏凋亡相关基因的表达，钼与镉呈协同关系。

关键词： 山羊; 镉; 钼; 自由基; 凋亡基因

本文引用格式

庄煜 , 王琦 , 曹华斌 , 胡国良 , 罗军荣 , 顾小龙 . 钼对镉胁迫下山羊血清抗氧化指标及肝脏凋亡相关基因表达的影响[J]. 动物营养学报, 2014 , 26(7) : 1822 -1829 . DOI: 10.3969/j.issn.1006-267x.2014.07.014

Abstract

This experiment was conducted to determine the effects of molybdenum (Mo) on serum anti-oxidative indices and hepatic apoptosis gene expressions of dairy goats challenged by cadmium (Cd). Thirty-six healthy Boer goats were randomly divided into 4 groups. Goats in test groups Ⅱ, Ⅲ and Ⅳ were all drenched 1 mg/kg BW Cd, and then were drenched 15, 30 and 45 mg/kg BW Mo, respectively, while those in test group Ⅰ were drenched demineralized water at the average dose of the other 3 groups. The experimental period was 50 days. Blood collection was carried out on days 0, 10, 20, 30, 40 and 50, then serum total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, nitric oxide (NO) content and NO synthase (NOS) activity were determined; liver tissue samples were obtained on days 0, 25 and 50 after slaughter, then hepatic Bax and Bcl-2 gene expression levels were determined. The results showed as follows: 1) compared with test group Ⅰ, serum T-AOC, SOD activity and hepatic Bcl-2 gene expression level could be significantly decreased in test groups Ⅱ, Ⅲ and Ⅳ (P<0.05), and serum NOS activity, MDA content, NO content and hepatic Bax gene expression level could be significantly increased in the above 3 groups (P<0.05); in addition, with the increase of experimental time, serum T-AOC, SOD activity and hepatic Bcl-2 gene expression level were significantly decreased (P<0.05), and serum MDA content, NO content, NOS activity and hepatic Bax gene expression level were significantly increased (P<0.05). 2) Compared among test groups Ⅱ, Ⅲ and Ⅳ, with the increase of Mo level, serum T-AOC and SOD activity were decreased, and serum MDA content, NO content and NOS activity and hepatic Bax gene expression level were increased when Cd were drenched at the same level. The results indicate that Mo and Cd can decrease serum antioxidant capacity and up-regulate the expressions of hepatic apoptosis genes, and they had a synergetic effect.

Key words： goat; cadmium; molybdenum; free radical; apoptosis gene

参考文献

[1] MARETTOVÁ E,MARETTA M,LEGÁTH J,et al.Changes in the peritubular tissue of rat testis after cadmium treatment[J].Biological Trace Element Research,2010,134(3):288-295.  
[2] 任继平,李德发,张丽英.镉毒性研究进展[J].动物营养学报,2003,15(1):1-6.
[3] HART B A,POTTS R J,WATKIN R D,et al.Cadmium adaptation in the lung-a double-edged sword?[J].Toxicology,2001,160(1/2/3):65-70.
[4] BERGLUND M,AKESSON A,BJELLERUP P,et al.Metal-bone interactions[J].Toxicology Letters,2000,112/113:219-225.
[5] 刘宗平,马卓,李文范,等.镉对绵羊的毒理学研究[J].畜牧兽医学报,1996,27(6):546-553.
[6] 樊璞.钼、镉环境污染对牛、猪、鸭的危害调查[J].中国兽医科技,1988(2):20-22.
[7] 王岗.绵羊矿物质营养研究的最新进展及其对中国养羊生产可能产生的影响[J].动物营养学报,1998,10(1):1-11.
[8] 潘翠玲,黄克和,赵玉鑫,等.不同硒源对蛋鸡组织硒含量及其抗氧化能力的影响[J].畜牧兽医学报,2010,41(12):1605-1613.
[9] SHIELLS R,FALK G.Retinal on-bipolar cells contain a nitric oxide-sensitive guanylate cyclase[J].Neuroreport,1992,3(10):845-848.  
[10] ROBERFROID M,CALDERON P B.Free radicals and oxidation phenomena in biological systems[M]. :CRC Press,1994.
[11] 杨原志,吴业阳,董晓慧,等.方斑东风螺饲料中锌需要量的研究[J].动物营养学报,2013,25(3):643-650.
[12] 杨自军,张才,王宏伟,等.锌、铜对实验性钼中毒绵羊肝脏、肾脏中钼含量的影响[J].中国兽医学报,2011,31(6):895-898.
[13] CAO J H,MA G Z,FANG Z Q.Effects of cadmium on the activity of enzymes in the gill and liver of the grass carp ctenopharyngodon idellus[J].Reservoir Fisheries,2004,24(1):9-11.
[14] OGNJANOVI ČĆBŘ I,PAVLOVI ĆS Z,MALETI ĆS D,et al.Protective influence of vitamin E on antioxidant defense system in the blood of rats treated with cadmium[J].Physiological Research,2003,52(5):563-570.
[15] OCHI T,TAKAHASHI K,OHSAWA M.Indirect evidence for the induction of a prooxidant state by cadmium chloride in cultured mammalian cells and a possible mechanism for the induction[J].Mutation Research,1987,180(2):257-266.  
[16] ZHOU J F,CAI D,TONG G Z.Oxidative stress and potential free radical damage associated with photocopying. A role for ozone?[J].Free Radical Research,2003,37(2):137-143.  
[17] MARTIN G M,LAMARCO K,STRAUSS E,et al.Research on aging:the end of the beginning[J].Science,2003,299(5611):1339-1341.  
[18] 孙存普.自由基生物学导论[M].合肥:中国科学技术大学出社,1999:236-255.
[19] CHOE W,KIM S,HWANG T S,et al.Expression of inducible nitric oxide synthase in thyroid neoplasms:immunohistochemical and molecular analysis[J].Pathology International,2003,53(7):434-439.  
[20] 张金波,郎庆玲,朱金玲,等.Bcl-2基因家族在调节细胞凋亡中的作用[J].中华中西医学杂志,2005,3(3):16-18.
[21] 于振刚,张居民,王炳辙.凋亡相关基因Bcl-2、Bax在NSCLC中的表达与细胞凋亡的关系[J].临床肺科杂志,2010,15(1):13-15.
[22] 刘志,郑军.Bcl-2家族蛋白及其在细胞凋亡中的作用[J].生命的化学,2007,27(1):22-25.
[23] 张明,袁慧.镉对小鼠生精细胞凋亡及bax和Bcl-2基因表达的影响[J].中国兽医科学,2007,37(3):251-254.
[24] 杨帆,崔恒敏,肖杰,等.高钼对艾维茵肉鸡胸腺细胞周期和凋亡影响的研究[J].畜牧兽医学报,2010,41(6):761-768.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2