反刍动物营养 Ruminant Nutrition

不同蛋白质源饲粮对肉牛能氮代谢和甲烷排放的影响

  • 张晓明 ,
  • 王之盛 ,
  • 唐春梅 ,
  • 陈慧 ,
  • 陈艳 ,
  • 邹华围 ,
  • 彭全辉
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  • 四川农业大学动物营养研究所, 雅安 625014

收稿日期: 2014-02-13

  网络出版日期: 2014-07-01

基金资助

农业部公益性行业专项饲料营养价值与畜禽饲养标准研究与应用(20090300608);青藏高原社区畜牧业行业科技项目(201203008)

Effects of Different Dietary Protein Sources on Energy and Nitrogen Metabolism and Methane Emission of Beef Cattle

  • ZHANG Xiaoming ,
  • WANG Zhisheng ,
  • TANG Chunmei ,
  • CHEN Hui ,
  • CHEN Yan ,
  • ZOU Huawei ,
  • PENG Quanhui
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  • Animal Nutrition Institute, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2014-02-13

  Online published: 2014-07-01

摘要

本试验旨在研究不同蛋白质源饲粮对肉牛能氮代谢和甲烷排放的影响。试验选用3头健康状况良好,体重接近并装有永久性瘤胃瘘管的宣汉黄牛,采用3×3完全拉丁方设计。试验饲粮分别以豆粕、菜籽粕、棉籽粕为蛋白质源进行配制;试验分3期,每期20 d,其中预试期10 d,消化试验3 d,呼吸代谢试验7 d。结果表明:1)各试验组的总挥发性脂肪酸浓度差异不显著(P>0.05),豆粕组和菜籽粕组的乙酸浓度显著高于棉籽粕组(P菜籽粕组>棉籽粕组;各组氨态氮含量差异显著(P棉籽粕组>菜籽粕组。2)豆粕组、棉籽粕组、菜籽粕组的采食氮、粪氮、可消化氮以及氮消化率差异都不显著(P>0.05);尿氮则是豆粕组和棉籽粕组显著高于菜籽粕组(P棉籽粕组>菜籽粕组,豆粕组的增重净能、增重净能/总能、增重净能/消化能和增重净能/代谢能均显著低于菜籽粕组和棉籽粕组(P菜籽粕组>棉籽粕组。综上所述,以菜籽粕为蛋白质源的饲粮氮生物学效价最高,豆粕次之,棉籽粕最低;以棉籽粕为蛋白质源的饲粮增重净能最高,菜籽粕次之,豆粕最低;以豆粕为蛋白质源的饲粮的甲烷排放量最高,菜籽粕次之,棉籽粕最低。

本文引用格式

张晓明 , 王之盛 , 唐春梅 , 陈慧 , 陈艳 , 邹华围 , 彭全辉 . 不同蛋白质源饲粮对肉牛能氮代谢和甲烷排放的影响[J]. 动物营养学报, 2014 , 26(7) : 1830 -1837 . DOI: 10.3969/j.issn.1006-267x.2014.07.015

Abstract

This study was conducted to study the effects of different dietary protein sources on energy and nitrogen metabolism and methane emission of beef cattle. Three healthy Xuanhan Yellow cattle with similar body weight fitted with permanent ruminal cannulas were used in a 3×3 complete Latin square design. Soybean meal, rapeseed meal and cottonseed meal were used as protein sources to prepare experimental diets; the experiment was consisted of 3 periods with 20 days in each period (pre-experimental period: 10 days; digestion experiment: 3 days; breath metabolism experiment: 7 days). The results showed as follows: 1) total volatile fatty acid concentration in experimental groups was not significantly different (P>0.05); acetate concentration in soybean meal and rapeseed meal groups was significantly higher than that in cottonseed meal group (Prapeseed meal group>cottonseed meal group; NH3-N content in three groups was significantly different (Prapeseed meal group > cottonseed meal group. 2) Nitrogen intake, fecal nitrogen, digestible nitrogen and nitrogen digestibility in three groups were not significant different (P>0.05); urinary nitrogen in soybean meal and cottonseed meal groups was significantly higher than that in rapeseed meal group (Pcottonseed group meal>rapeseed meal group; net energy for gain (NEg), NEg/gross energy, NEg/digestive energy and NEg/metabolizable energy in soybean meal group were significantly lower than those in rapeseed meal and cottonseed meal groups (Prapeseed meal group>cottonseed group meal. It is concluded that nitrogen biological value of the diet with rapeseed meal as the protein source is the highest, that with soybean meal is lower, and that with cottonseed meal is the lowest; NEg of the diet with cottonseed meal as the protein source is the highest, that with rapeseed meal is lower, and that with soybean meal is the lowest; methane emission of the diet with soybean meal as protein source is the highest, that with rapeseed meal is lower, and that with cottonseed meal is the lowest.

参考文献

[1] MACRAE J C.Advancing our understanding of amino-acid utilization and metabolism in ruminant tissues //KORNEGAY E T.Nutrient management of food animals to enhance and protect the environment.Boca Raton:CRC Press Inc.,1996:73-89.
[2] 乔岩瑞.养殖业氮污染分析和营养学调控[J].饲料研究,2006(10):21-24.
[3] MULLIGAN F J,DILLON P,CALLAN J J,et al.Supplementary concentrate type affects nitrogen excretion of grazing dairy cows[J].Journal of Dairy Science,2004,87(10):3451-3460.  
[4] 俞联平,张林.反刍家畜安全性无公害生产的饲料安全对策[J].中国乳业,2002(9):23-26.
[5] BARGO F,MULLER L D,VARGA G A,et al.Ruminal digestion and fermentation of high-producing dairy cows with three different feeding systems combining pasture and total mixed rations[J].Journal of Dairy Science,2002,85(11):2964-2973.  
[6] 李胜利,金鑫,范学珊,等.反刍动物生产与碳减排措施[J].动物营养学报,2010,22(1):2-9.
[7] 娜仁花.不同日粮对奶牛瘤胃甲烷及氮排放的影响研究 .博士学位论文.北京:中国农业科学院,2010.
[8] LODMAN D W,BRANINE M E,CARMEAN B R,et al.Estimates of methane emissions from manure of US cattle[J].Chemosphere,1993,26(1/2/3/4):189-199.
[9] 李新建,高腾云.影响瘤胃内甲烷气产量的因素及其控制措施[J].家畜生态,2002,23(4):67-69.
[10] 娜仁花,董红敏.营养因素对反刍动物甲烷排放影响的研究现状[J].安徽农业科学,2009,37(6):2534-2536.
[11] MOSS A R,GIVENS D I.The effect of supplementing grass silage with soya bean meal on digestibility,in sacco degradability,rumen fermentation and methane production in sheep[J].Animal Feed Science and Technology,2002,97(3/4):127-143.
[12] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.
[13] ERWIN E S,MARCO G J,EMERY E M.Volatile fatty acid analyses of blood and rumen fluid by gas chromatography[J].Journal of Dairy Science,1961,44(9):1768-1771.  
[14] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].内蒙古畜牧科学,1993(4):40-41.
[15] BLAXTER K L.The energy metabolism of ruminants[M].London:Hutchinson Scientific and Technical,1962.
[16] 杨占山.SF-6示踪法测定荷斯坦奶牛能量代谢的研究 .硕士学位论文.泰安:山东农业大学,2010.
[17] 王海荣,侯先志,王贞贞,等.日粮不同蛋白源对绵羊瘤胃发酵功能的影响[J].河北农业大学学报,2009,32(5):88-93.
[18] CORLEY Ⅲ R N,MURPHY M R.An in vitro technique for measuring the production rate of volatile fatty acids in the rumen under dynamic conditions[J].Small Ruminant Research,2004,54(3):219-225.  
[19] 高秀华,王峰,赵景辉,等.日粮蛋白质水平对梅花鹿瘤胃内pH值、NH3-N和VFA浓度的影响[J].动物营养学报,1996,8(4):27-33.
[20] 王潍波,赵国琦,王瑞龙,等.不同蛋白日粮对山羊瘤胃环境参数的影响[J].饲料工业,2008,29(20):44-48.
[21] 李旺.瘤胃挥发性脂肪酸的作用及影响因素[J].中国畜牧杂志,2012,48(7):63-66.
[22] 杨红建,冯仰廉.不同纤维素与淀粉比率等氮纯化日粮瘤胃发酵挥发性脂肪酸产生量[J].中国畜牧杂志, 2003,39(5):9-10.
[23] 胡红莲,卢德勋,刘大程,等.日粮不同NFC/NDF比对奶山羊瘤胃pH,挥发性脂肪酸及乳酸含量的影响[J].动物营养学报,2010,22(3):595-601.
[24] TAMMINGA S.Nutrition management of dairy cows as a contribution to pollution control[J].Journal of Dairy Science,1992,75(1):345-357.  
[25] 翟少伟.日粮蛋白质摄入量对泌乳奶牛氮排泄量的影响[J].中国奶牛,2009(8):56-59.
[26] GRESSLEY T F,ARMENTANO L E.Effect of abomasal pectin infusion on digestion and nitrogen balance in lactating dairy cows[J].Journal of Dairy Science,2005,88(11):4028-4044.  
[27] FLIS S A,WATTIAUX M A.Effects of parity and supply of rumen-degraded and undegraded protein on production and nitrogen balance in Holsteins[J].Journal of Dairy Science,2005,88(6):2096-2106.  
[28] 陈代文.动物营养与饲料学[M].北京:中国农业出版社,2005.
[29] 中华人民共和国农业部.肉牛饲养标准NY/T815-2004[S].北京:中国农业出版社,2004.
[30] 徐红蕊,时建青,赵国琦.反刍动物体内尿素氮代谢研究进展[J].畜牧兽医杂志,2006,25(1):23-24,27.
[31] 杨凤.动物营养学[M].北京:中国农业出版社,1993.
[32] WOLIN M J,MILLER T L.Microbe interactions in the rumen microbial ecosystem[M].HOBSON P N.The rumen microbial ecosystem.London:Elsevier Applied Science,1988:343-359.
[33] 韩继福,冯仰廉,张晓明,等.阉牛不同日粮的纤维消化、瘤胃内VFA对甲烷产生量的影响[J].中国兽医学报,1997,17(3):278-280.
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