动物营养学报 >

2014 , Vol. 26 >Issue 7: 1864 - 1872

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.07.019

水产营养 Aquaculture Nutrition

养成期异育银鲫对饲料赖氨酸的需求量

  • 王鑫 ,
  • 薛敏 ,
  • 王嘉 ,
  • 郑银桦 ,
  • 吴秀峰 ,
  • 韩芳 ,
  • 吴立新
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  • 1. 大连海洋大学水产与生命学院, 大连 116023;
    2. 中国农业科学院饲料研究所, 国家水产饲料安全评价基地, 北京 100081

收稿日期: 2014-02-17

  网络出版日期: 2014-07-01

基金资助

公益性行业（农业）科研专项经费项目（201003020，201203015）；国家自然科学基金（31372539，31101907）

收起

Dietary Lysine Requirement of Grow-Up Gibel Carp (Carassiusauratus gibeilo)

  • WANG Xin ,
  • XUE Min ,
  • WANG Jia ,
  • ZHENG Yinhua ,
  • WU Xiufeng ,
  • HAN Fang ,
  • WU Lixin
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  • 1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
    2. The National Aquatic Feed Safety Assessment Center, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2014-02-17

  Online published: 2014-07-01

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摘要

本试验旨在确定养成期异育银鲫对饲料赖氨酸的需求量。以实用饲料配方为基础，以L-赖氨酸盐酸盐（L-Lys·HCl）为赖氨酸来源，并以混合晶体氨基酸平衡其他必需氨基酸，配制赖氨酸水平分别为1.01%、1.23%、1.47%、1.70%、1.93%和2.21%的6种试验饲料。试验选择初重为（52.53±0.03） g的异育银鲫480尾，随机分为6组，每组4个重复，每个重复20尾鱼。试验期为59 d。结果表明：1.23%~2.21%组的增重率和特定生长率显著高于1.01%组（P0.05）。1.70%组全鱼水分含量显著低于1.93%、2.21%组（P0.05）。饲料赖氨酸水平显著影响肝胰脏谷草转氨酶活性（P0.05）。饲料赖氨酸水平对鲜肉的硬度、弹性和咀嚼性及熟肉的硬度和咀嚼性有显著影响（P<0.05）。以特定生长率、蛋白质沉积率和肝胰脏谷草转氨酶活性为指标进行二次回归分析，得出养成期异育银鲫对饲料赖氨酸的需求量分别为1.78%、1.43%和1.51%（分别占饲料蛋白质的5.14%、4.14%和4.44%）。

关键词： 异育银鲫; 赖氨酸; 需求量; 生长; 转氨酶; 肌肉品质

本文引用格式

王鑫 , 薛敏 , 王嘉 , 郑银桦 , 吴秀峰 , 韩芳 , 吴立新 . 养成期异育银鲫对饲料赖氨酸的需求量[J]. 动物营养学报, 2014 , 26(7) : 1864 -1872 . DOI: 10.3969/j.issn.1006-267x.2014.07.019

Abstract

A 59-day growth trial was conducted to determine the dietary lysine (Lys) requirement of grow-up gibel carp (Carassiusauratus gibeilo). On the basis of practical diet formulation, six isonitrogenous experimental diets were formulated containing 1.01%, 1.23%, 1.47%, 170%, 1.93% and 2.21% Lys, respectively. L-Lys·HCl was Lys source and essential amino acid crystal mixture was supplied in diets to balance the other essential amino acid requirement except Lys for gibel carp. A total of 480 gibel carp with an initial body weight of (52.53±0.03) g were chosen and randomly distributed into 6 groups with 4 replicates per group and 20 fish per replicate. The results showed as follows: the weight gain rate (WGR) and specific growth rate (SGR) in 1.23% to 2.21% groups were significantly higher than those in 1.01% group (P0.05). The moisture content in whole body was significantly lower, while the ash content was significantly higher in 1.70% group than those in 1.93% and 2.21% groups (P0.05). Dietary Lys level had significant effects on the activity of hepatopancreas aspartate aminotransferase (AST), and showed a trend of first increase and then down with the dietary Lys level increasing, while the activity of hepatopancreas alanine transarninase (ALT) was not affected by dietary Lys level (P>0.05). Dietary Lys level significantly affected the hardness, springiness and chewiness of fresh meat, and the hardness and chewiness of cooked meat (P<0.05). Using SGR, PRR and hepatopancreas AST activity as indices, quadratic regression analysis indicate the dietary Lys requirements of grow-up gibel carp are 1.78%, 1.43% and 1.51%, respectively (5.14%, 4.14% and 4.44% of dietary protein, respectively).

Key words： gibel carp (Carassiusauratus gibeilo); lysine; requirement; growth; transaminase; muscle quality

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