饲料营养 Feed Science and Technology

新型耐热植酸酶对断奶仔猪钙磷代谢及养分表观消化率的影响

  • 曹贵彬 ,
  • 熊昊 ,
  • 游金明 ,
  • 于会民 ,
  • 韦启鹏 ,
  • 邹志恒 ,
  • 叶亚玲
展开
  • 1. 江西农业大学动物科技学院, 南昌 330045;
    2. 中国农业科学院饲料研究所, 北京 100081;
    3. 江西省农业科学院畜牧兽医研究所, 南昌 330200

收稿日期: 2014-02-23

  网络出版日期: 2014-07-01

基金资助

“十二五”农村领域国家科技计划(2013BAD10B01);“十二五”国家科技支撑计划(2011BAD26B03)

New Heat-Resistant Phytase:Effects on Calcium and Phosphorus Metabolism and Apparent Nutrient Digestibility of Weaner Piglets

  • CAO Guibin ,
  • XIONG Hao ,
  • YOU Jinming ,
  • YU Huimin ,
  • WEI Qipeng ,
  • ZOU Zhiheng ,
  • YE Yaling
Expand
  • 1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
    2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    3. Institute of Animal Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China

Received date: 2014-02-23

  Online published: 2014-07-01

摘要

本研究旨在探讨低磷饲粮中添加新型耐热植酸酶替代一部分磷酸氢钙对断奶仔猪钙磷代谢及养分表观消化率的影响。试验选用256头遗传背景相同、初始体重为(8.37±0.97) kg的30日龄断奶仔猪,按完全随机区组设计分为4个处理,每个处理设4个重复,每个重复16头仔猪。4个处理分别饲喂基础饲粮、低磷饲粮、低磷饲粮基础上添加500 U/kg新型耐热植酸酶和低磷饲粮基础上添加1 000 U/kg新型耐热植酸酶的试验饲粮。试验期30 d。结果表明:1)各处理之间血清钙、磷含量及碱性磷酸酶活性差异均不显著(P>0.05),但添加有新型耐热植酸酶的试验组仔猪血清钙、磷含量有升高的趋势。2)在低磷饲粮中添加500、1 000 U/kg新型耐热植酸酶一定程度上提高了蹄骨灰分率和钙、磷含量,但各处理间差异不显著(P>0.05)。3)将仔猪趾骨制成骨骼切片后进行组织学观察,结果发现500、1 000 U/kg新型耐热植酸酶添加组趾骨骨密质较基础饲粮组和低磷饲粮组厚,骨松质较基础饲粮组和低磷饲粮组密集。4)基础饲粮组和新型耐热植酸酶添加组的钙、磷、能量及干物质的表观消化率显著高于低磷饲粮组(P<0.05)。由此可知,断奶仔猪饲粮中添加新型耐热植酸酶替代部分无机磷,能有效促进仔猪机体对饲粮中钙、磷的代谢,并显著提高饲粮中部分养分的表观消化率。综合各项指标,1 000 U/kg的新型耐热型植酸酶能起到更好的替代无机磷的作用。

本文引用格式

曹贵彬 , 熊昊 , 游金明 , 于会民 , 韦启鹏 , 邹志恒 , 叶亚玲 . 新型耐热植酸酶对断奶仔猪钙磷代谢及养分表观消化率的影响[J]. 动物营养学报, 2014 , 26(7) : 1900 -1907 . DOI: 10.3969/j.issn.1006-267x.2014.07.023

Abstract

This experiment was conducted to study the effects of new heat-resistant phytase (NHP) on the metabolism of calcium (Ca) and phosphorus (P) and apparent nutrient digestibility in weaner piglets by replacing part of calcium hydro phosphate by NHP in low-phosphorus diet. A total of 256 piglets weaned at 30 days of age and with the similar genetic background were randomly allotted to 4 treatments with 4 replicates per treatment and 16 piglets per replicate using a randomized complete-block design. They were fed basal diet, low-phosphorus diet, low-phosphorus diet+500 U/kg NHP, and low-phosphorus diet+1 000 U/kg NHP, respectively. The experiment lasted for 30 d. The results showed as follows: 1) there was no significant difference in serum Ca and P contents and alkaline phosphatase (ALP) activity among all treatments (P>0.05). However, serum Ca and P in the NHP treatments had tendencies of going upward. 2) Compared with low-phosphorus diet, the supplementation of 500 or 1 000 U/kg NHP improved Ca and P contents and ash ratio in coffin bone, but there were no differences among all treatments (P>0.05). 3) The phalanx section of piglets fed the diets supplemented with NHP showeded thicker compact bone and higher cancellous bone than those in basal diet group and low-phosphorus diet group. 4) The apparent digestibility of gross energy (GE), Ca, total phosphorus (TP), and dry matter (DM) in basal diet group, 500 U/kg NHP group and 1 000 U/kg NHP group were higher than those in basal diet group and low-phosphorus diet group (P<0.05). In conclusion, by using NPH to replace part of P in diets can improve the metabolism of Ca and P in piglets as well as the apparent digestibility of some nutrients. In addition, 1 000 U/kg NHP is an appropriate choice to replace phosphorus in diet.

参考文献

[1] 罗专溪,朱波,唐家良,等.自然沟渠控制村镇降雨径流中氮磷污染的主要作用机制[J].环境科学学报,2009,29(3):561-568.
[2] 李光辉.微量元素间及其与营养素之间的协同与拮抗作用[J].乳业科学与技术,2005,27(2):80-82.
[3] SIMONS P C M,VERSTEEGH H A J,JONGBLOED A W,et al.Improvement of phosphorus availability by microbial phytase in broilers and pigs[J].British Journal of Nutrition,1990,64(2):525-540.  
[4] JONGBLOED A W,KEMME P A.Effect of pelleting mixed feeds on phytase activity and the apparent absorbability of phosphorus and calcium in pigs[J].Animal Feed Science and Technology,1990,28(3/4):233-242.
[5] DENBOW D M,RAVINDRAN V,KORNEGAY E T,et al.Improving phosphorus availability in soybean meal for broilers by supplemental phytase[J].Poultry Science,1995,74(11):1831-1842.  
[6] 韩进诚,杨晓丹,杨凤霞,等.微生物植酸酶与1-21日龄肉鸡饲粮无机磷当量模型研究[J].畜牧兽医学报,2008,39(7):907-914.
[7] 周佳萍.耐热植酸酶对植酸降解及肉鸡生产性能、矿物元素利用率影响的研究 .硕士学位论文.泰安:山东农业大学,2008.
[8] CROMWELL G L,STAHLY T S,COFFEY R D,et al.Efficacy of phytase in improving the bioavailability of phosphorus in soybean meal and corn-soybean meal diets for pigs[J].Journal of Animal Science,1993,71(7):1831-1840.
[9] RAVINDRAN V,BRYDEN W L,KORNEGAY E T.Phytates:occurrence, bioavailability and implications in poultry nutrition[J].Poultry and Avian Biology Reviews,1995,6(2):125-143.
[10] BROZ J,OLDALE P,PERRIN-VOLTZ A H,et al.Effects of supplemental phytase on performance and phosphorus utilisation in broiler chickens fed a low phosphorus diet without addition of inorganic phosphates[J].British Poultry Science,1994,35(2):273-280.  
[11] 徐奇友,金明玉,孙玉红,等.植酸酶对肉仔鸡生产性能及钙、磷利用率影响的研究[J].动物营养学报,2000,12(1):26-31.
[12] VARLEY P F,CALLAN J J,O'DOHERTY J V.Effect of phosphorus level and phytase inclusion on the performance, bone mineral concentration, apparent nutrient digestibility, and on mineral and nitrogen utilisation in finisher pigs[J].Irish Journal of Agricultural and Food Research,2010,49(2):141-152.
[13] KUMAR V,SINHA A K,MAKKAR H P S,et al.Phytate and phytase in fish nutrition[J].Journal of Animal Physiology and Animal Nutrition,2012,96(3):335-364.  
[14] 冯定远.酶制剂在饲料工业中的应用[M].北京:中国农业科学技术出版社,2005:348-353.
[15] BEHNAM B N,YAHYA E N,MASOUD S,et al.Effects of dietary available phosphorus and microbial phytase on growth performance,carcass traits,serum minerals and toe ash content in broiler chicks[J].International Journal of Agriculture and Biology,2012,14(3):435-439.
[16] 刘宗平,曲亚玲,马小军,等.双峰驼骨质疏松症的研究[J].中国农业科学,2003,36(2):214-217.
[17] 齐宗利,陈耀明,吴瑞宗,等.兔应力性骨折发生过程中钙磷锌元素的改变[J].微量元素与健康研究,2004,21(4):1-3.
[18] MA Z J,YAMAGUCHI M.Role of endogenous zinc in the enhancement of bone protein synthesis associated with bone growth of newborn rats[J].Journal of Bone and Mineral Metabolism,2001,19(1):38-44.  
[19] 蔡青和,计成,岳洪源.玉米豆粕型日粮中添加植酸酶对断奶仔猪生产性能、养分消化率及血清生化指标的影响[J].动物营养学报,2004,16(2):15-21.
[20] 梁陈冲,陈宝江,于会民,等.不同来源植酸酶对猪生长性能、营养物质表观消化率及肠道微生物区系的影响[J].动物营养学报,2013,25(11):2705-2712.
[21] KYRIAZAKIS I,EMMANS G C.The effects of varying protein and energy intakes on the growth and body composition of pigs[J].British Journal of Nutrition,1992,68(3):615-625.  
[22] 楼洪兴,许松,吴建良,等.糠麸棉粕日粮中植酸酶添加水平对肉鸡生长性能和磷利用的影响[J].畜牧兽医学报,2000,31(1):16-21.
[23] CENTENO C,ARIJA I,VIVEROS A,et al.Effects of citric acid and microbial phytase on amino acid digestibility in broiler chickens[J].British Poultry Science,2007,48(4):469-479.  
[24] 董其国,徐稳,王恬.不同剂型植酸酶对育肥猪饲料养分利用率的影响[J].饲料研究,2010(3):6-9.
[25] 高俊杰,车向荣.饲粮中添加微生物植酸酶和有机酸对断奶仔猪生产性能的影响[J].饲料工业,2006,27(24):21-23.
[26] QIAN H,KORNEGAY E T,CONNER D E,Jr.Adverse effects of wide calcium:phosphorus ratios on supplemental phytase efficacy for weanling pigs fed two dietary phosphorus levels[J].Journal of Animal Science,1996,74(6):1288-1297.
文章导航

/