研究简报 Short Communications

热处理大豆分离蛋白质饲粮对肉鸡胰腺功能的影响

  • 陈星 ,
  • 吴大伟 ,
  • 温超 ,
  • 周岩民
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  • 南京农业大学动物科技学院, 南京 210095

收稿日期: 2014-01-29

  网络出版日期: 2014-07-01

基金资助

江苏省普通高校研究生科研创新计划项目(CXLX13-289)

Effects of Heat Treatment of Soy Protein Isolate on Pancreatic Function of Broilers

  • CHEN Xing ,
  • WU Dawei ,
  • WEN Chao ,
  • ZHOU Yanmin
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  • College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China

Received date: 2014-01-29

  Online published: 2014-07-01

摘要

本试验旨在探讨热处理大豆分离蛋白质饲粮对肉鸡胰腺功能的影响。选用1日龄爱拔益加(AA)肉鸡320只,随机分为4个组,每组8个重复,每个重复10只,分别饲喂4种饲粮:正常大豆分离蛋白质饲粮(对照组),100℃热处理1、4及8 h的大豆分离蛋白质饲粮,试验期为21 d。结果表明:与对照组相比,各热处理大豆分离蛋白质饲粮对14日龄肉鸡胰腺功能相关指标均无显著影响(P>0.05);21日龄,各热处理大豆分离蛋白质饲粮均显著提高了肉鸡胰腺的相对重量(P<0.05),热处理8 h大豆分离蛋白质饲粮使肉鸡胰腺胰蛋白酶和蛋白酶活性显著降低(P<0.05);热处理8 h组肉鸡胰腺丙二醛含量显著升高(P<0.05),氧化型谷胱甘肽含量有升高趋势(P=0.065),但总抗氧化能力(P=0.058)及过氧化氢酶(P=0.064)、谷胱甘肽还原酶(P=0.061)活性有降低的趋势;此外,热处理8 h大豆分离蛋白质饲粮使胰腺中还原型谷胱甘肽/氧化型谷胱甘肽和谷胱甘肽过氧化物酶活性显著下降(P<0.05)。试验结果提示,摄食热处理大豆分离蛋白质饲粮一定时间后会对肉鸡胰腺功能产生负面作用。

本文引用格式

陈星 , 吴大伟 , 温超 , 周岩民 . 热处理大豆分离蛋白质饲粮对肉鸡胰腺功能的影响[J]. 动物营养学报, 2014 , 26(7) : 1942 -1949 . DOI: 10.3969/j.issn.1006-267x.2014.07.028

Abstract

This experiment was conducted to investigate the effects of heat treatment soy protein isolate (SPI) on pancreatic function of broilers. A total of 320 one-day-old Arbor Acres broilers were randomly divided into 4 groups with 8 replicates per group and 10 broilers per replicate. Broilers were fed a diet contained normal SPI (control group), or diets contained SPI heated in an oven at 100℃ for 1, 4, and 8 h, respectively. The experiment lasted for 21 days. The results showed that compared with the control group, there were no significant differences in pancreatic function parameters of broilers fed diets contained heated SPI at 14 days of age (P>0.05). The pancreas relative weight of broilers fed diets contained heated SPI was significantly higher than that in control group at 21 days of age (P<0.05). The activities of protease and trypsin in pancreas were significantly lower in broilers fed diets contained heated SPI heated for 8 h than those in control group (P<0.05). Broilers fed diets contained SPI heated for 8 h exhibited a decrease in total antioxidant capacity (T-AOC) (P=0.058) and catalase (CAT) activity (P=0.064) compared with the control group at 21 days of age. Malondialdehyde (MDA) content of birds fed diets contained SPI heated for 8 h was significantly higher than that in control group at the same time (P<0.05). In addition, a tendency toward an increase in glutathione disulphide (GSSG) content (P=0.065) and an decrease in glutathione reductase (GR) activity (P=0.061) in broilers fed diets contained SPI heated for 8 h was observed at 21 days of age. Birds fed diets contained SPI heated for 4 and 8 h showed a significantly decrease in the ratio of reduced glutathione (GSH) to GSSG and glutathione peroxidase (GPx) activity than those in control group at 21 days of age (P<0.05). It is concluded that heated SPI ingestion after a specified period can increase the damage of pancreas function.

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