动物营养学报 >

2014 , Vol. 26 >Issue 7: 1950 - 1961

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.07.029

研究简报 Short Communications

不同硒源及水平对肉鸡生长性能、血浆和组织硒含量及血浆谷胱甘肽过氧化物酶活性的影响

  • 郭军蕊 ,
  • 刘国华 ,
  • 郑爱娟 ,
  • 常文环 ,
  • 张姝 ,
  • 蔡辉益
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  • 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081

收稿日期: 2014-02-21

  网络出版日期: 2014-07-01

基金资助

国家科技支撑计划课题（2011BAD26B03）

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Effects of Selenium Sources and Supplemental Levels on the Growth Performance, Plasma and Tissue Selenium Content and Plasma Glutathione Peroxidase Activity of Broilers

  • GUO Junrui ,
  • LIU Guohua ,
  • ZHENG Aijuan ,
  • CHANG Wenhuan ,
  • ZHANG Shu ,
  • CAI Huiyi
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  • Ministry of Agriculture Key Open Laboratory of Feed Biotechnology, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2014-02-21

  Online published: 2014-07-01

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摘要

本文主要以低硒基础饲粮作为负对照，亚硒酸钠、酵母硒为正对照，研究蛋氨酸硒对肉鸡生长性能、血浆和组织硒含量及血浆谷胱甘肽过氧化物酶（GSH-Px）活性的影响。本试验选用756只1日龄爱拔益加（AA）健康肉雏鸡随机分为7组，每组6个重复，每个重复18只鸡。组1为负对照组，采用低硒基础饲粮；组2~7饲粮分别在低硒基础饲粮中添加0.1、0.3、0.5、0.7 mg/kg蛋氨酸硒、0.3 mg/kg酵母硒（正对照组）和0.3 mg/kg亚硒酸钠（正对照组），试验期为42 d。结果表明：各组肉鸡平均日增重、平均日采食量、料重比、初体重、末体重及死淘率无显著差异（P>0.05）；但对于不同日龄血浆硒含量，各加硒组均显著高于负对照组（P<0.05），且随着硒添加水平的增加而增大，相同添加水平蛋氨酸硒组优于2个正对照组，日龄和硒添加水平之间存在极显著交互作用（P<0.01）；对于不同日龄不同硒水平间血浆GSH-Px活性，各加硒组显著高于负对照组（P<0.05），且0.1 mg/kg加硒组GSH-Px活性显著低于其他各加硒组（P<0.05），日龄和添加水平之间存在极显著交互作用（P<0.01）；对于42日龄肝脏、肾脏、肌肉、皮肤和腹脂硒含量，各加硒组随添加水平的增加而增大，且均显著高于负对照组（P<0.05），相同添加水平蛋氨酸硒组优于2个正对照组。综上，饲粮中添加亚硒酸钠、酵母硒和蛋氨酸硒对肉鸡生长性能无明显改善，但提高了血浆GSH-Px活性和血浆及各组织硒的含量，尤其对于组织硒的含量，蛋氨酸硒优于亚硒酸钠和酵母硒。

关键词： 肉鸡; 生长性能; 血浆和组织硒含量; GSH-Px活性

本文引用格式

郭军蕊 , 刘国华 , 郑爱娟 , 常文环 , 张姝 , 蔡辉益 . 不同硒源及水平对肉鸡生长性能、血浆和组织硒含量及血浆谷胱甘肽过氧化物酶活性的影响[J]. 动物营养学报, 2014 , 26(7) : 1950 -1961 . DOI: 10.3969/j.issn.1006-267x.2014.07.029

Abstract

The purpose of this study was to investigate the effects of selenomethionine (Se-Met) on growth performance, plasma and tissue selenium (Se) content and plasma glutathione peroxidase (GSH-Px) activity of broilers, based on low selenium diet as a negative control, sodium selenite and selenium-enriched yeast (Se-yeast) as positive control. Seven hundred and fifty-six 1-day-old Arbor Acres (AA) broilers were randomly allocated into 7 groups with 6 replicates in each group and 18 broilers per replicate. Group 1 was the negative control group, and a low selenium basal diet was used. The diets of groups 2 to 7 were added 0.1, 0.3, 0.5, 0.7 mg/kg Se-Met, 0.3 mg/kg sodium selenite (positive control group) and 0.3 mg/kg Se-yeast (positive control group) in the low selenium basal diet, respectively. The experiment lasted for 42 d. The results showed that there were no significant differences in average daily gain (ADG), average daily feed intake (ADFI), feed/gain (F/G), initial weight, final weight and mortality of boilers (P>0.05). However, compared with the negative control group, the supplementation of Se significantly increased Se content in plasma of broilers at different days of age (P<0.05). Se content in plasma was increased with the increase of Se supplemental level, and the effects of Se-Met were superior to thoae of the 2 positive control groups on the same supplemental level. There was a significant interaction between days of age and Se supplemental level about Se content in plasma (P<0.01). GSH-Px activity in plasma was significantly higher in Se supplemental groups compared with negative control group at different days of age (P<0.05). Among the Se supplemental groups, GSH-Px activity of the 0.1 mg/kg Se group was lower than that of the other groups (P<0.05). There was significant interaction between days of age and Se supplemental level (P<0.01). Compared with negative control group, the supplementation of Se significantly increased Se content in liver, kidney, muscle, skin and fat (P<0.05). The Se content in tissues was increased with the supplemental level of Se increased. The effects of Se-Met supplemental groups were superior to 2 positive control groups at the same supplemental level. In conclusion, adding organic Se or inorganic Se can't improve growth performance of broilers. However, it can increase GSH-Px activity and Se content of plasma and tissue, especially, in terms of tissue Se content, and Se-Met is superior to sodium selenite and Se-yeast.

Key words： broiler; growth performance; plasma and tissue selenium content; GSH-Px activity

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