反刍动物营养 Ruminant Nutrition

饲粮中性洗涤纤维与非纤维性碳水化合物比例对湖羊瘤胃还原硝态氮的影响

  • 林淼 ,
  • 张建刚 ,
  • 陈志远 ,
  • 赵国琦
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  • 扬州大学动物科学与技术学院, 扬州 225009

收稿日期: 2014-09-17

  网络出版日期: 2014-12-05

基金资助

国家自然科学基金(31301997);江苏高校优势学科建设工程资助项目

Effects of Dietary Neutral Detergent Fiber to Non-Fiber Carbohydrates Ratio on Ruminal Nitrate Reduction of Hu Sheep

  • LIN Miao ,
  • ZHANG Jiangang ,
  • CHEN Zhiyuan ,
  • ZHAO Guoqi
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  • College of Animal Sciences and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2014-09-17

  Online published: 2014-12-05

摘要

本试验采用4×4拉丁方试验设计,研究4种中性洗涤纤维与非纤维性碳水化合物比例(NDF/NFC)的饲粮(0.58、0.71、1.20、1.57)对湖羊瘤胃硝态氮含量动态变化和发酵特性的影响.各组饲粮中均含有1.0%硝酸钾,在喂后0、0.5、1.0、1.5、2.0、4.0、6.0、8.0 h通过瘤胃瘘管采集瘤胃液,测定硝酸盐和亚硝酸盐含量以及瘤胃发酵参数;晨饲后2.0 h通过颈静脉采血,测定高铁血红蛋白含量.结果表明,6.0~8.0 h饲粮NDF/NFC=0.71时瘤胃亚硝酸盐含量最低;且0~1.5 h随着NDF/NFC的增大,氨态氮(NH3-N)含量有逐渐降低的趋势;0、8.0 h总微生物蛋白质含量以NDF/NFC=0.58时最高,且与NDF/NFC=0.71时差异不显著(P>0.05),整个试验期未出现亚硝酸盐中毒.因此,在本试验条件下,湖羊饲粮NDF/NFC=0.71时,最有利于瘤胃硝态氮还原及瘤胃发酵.

本文引用格式

林淼 , 张建刚 , 陈志远 , 赵国琦 . 饲粮中性洗涤纤维与非纤维性碳水化合物比例对湖羊瘤胃还原硝态氮的影响[J]. 动物营养学报, 2014 , 26(12) : 3659 -3665 . DOI: 10.3969/j.issn.1006-267x.2014.12.015

Abstract

A 4 × 4 Latin square design in this study was used to investigate the effects of different dietary neutral detergent fiber to non-fiber carbohydrates ratios (NDF/NFC) (0.58, 0.71, 1.20 and 1.57) on dynamic changes of ruminal nitrate nitrogen content and fermentation characteristics of Hu sheep. The diet of each group contained 1.0% potassium nitrate, and rumen fluid was obtained at 0, 0.5, 1.0, 1.5, 2.0, 4.0, 6.0 and 8.0 h after feeding to determine the contents of nitrate and nitrite and ruminal fermentation parameters. Blood sample was collected at 2.0 h after feeding to determine the blood methemoglobin content. The results showed that the nitrite content of sheep fed diet with NDF/NFC=0.71 was lowest at 6.0 to 8.0 h after feeding. With the rising of NDF/NFC, NH3-N content had a trend of decrease at 0 to 1.5 h after feeding. Total microbial protein content of sheep fed diet with NDF/NFC=0.58 was highest at 0 and 8.0 h after feeding, and had no significant difference with sheep fed diet with NDF/NFC=0.71 (P>0.05). In addition, the nitrite toxicity was not detected during experiment period. In conclusion, the optimum NDF/NFC in the diet of Hu sheep is 0.71 for nitrate nitrogen reduction and fermentation in the rumen under this experimental condition.

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