动物营养学报 >

2014 , Vol. 26 >Issue 12: 3731 - 3739

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.12.024

饲料营养 Feed Science and Technology

甜菜碱对热应激肉鸡生长性能、十二指肠消化酶活性及盲肠微生物区系的影响

  • 贺绍君 ,
  • 赵书景 ,
  • 李静 ,
  • 车传燕 ,
  • 戴四发 ,
  • 刘德义
展开
  • 安徽科技学院动物科学学院, 凤阳 233100

收稿日期: 2014-06-07

  网络出版日期: 2014-12-05

基金资助

安徽省教育厅自然科学重点项目(KJ2012A068);安徽省教育厅自然科学一般项目(KJ2013B081);安徽省自然科学面上项目(1408085MC62);安徽科技学院自然科学基金项目(ZRC2013334)

收起

Effects of Betaine on Growth Performance, Activities of Duodenum Digestive Enzymes and Cecal Microflora of Heat-Stressed Broilers

  • HE Shaojun ,
  • ZHAO Shujing ,
  • LI Jing ,
  • CHE Chuanyan ,
  • DAI Sifa ,
  • LIU Deyi
Expand
  • College of Animal Science and Techonology, Anhui Science and Technology University, Fengyang 233100, China

Received date: 2014-06-07

  Online published: 2014-12-05

Fold

摘要

本试验通过在饲粮中添加不同剂量的甜菜碱研究其对热应激肉鸡生长性能、十二指肠消化酶活性及盲肠微生物区系的影响.选取270只14日龄爱拔益加(AA)肉鸡随机分成6组:常温组、热应激组,常温+0.1%甜菜碱组、热应激+0.1%甜菜碱组、热应激+0.2%甜菜碱、热应激+0.4%甜菜碱组.热应激肉鸡饲养在(32±1) ℃的高温环境中.每组3个重复,每个重复15只.预试期7 d,正试期21 d.结果表明:1)饲粮添加甜菜碱可极显著增加28~42日龄热应激肉鸡的体重(P<0.01),极显著降低22~35日龄肉鸡料重比(P<0.01);2)除添加0.1%和0.2%甜菜碱饲粮对28日龄热应激肉鸡胰蛋白酶活性无显著影响(P>0.05)和添加0.1%甜菜碱对35日龄热应激肉鸡淀粉酶活性无显著影响(P>0.05)外,不同浓度的甜菜碱均能显著或极显著增加28、35、42日龄热应激肉鸡十二指肠胰蛋白酶、淀粉酶和胰脂肪酶活性(P<0.05或P<0.01);3)饲粮添加甜菜碱极显著提高了28、35、42日龄热应激肉鸡盲肠内乳酸杆菌的数量(P<0.01),极显著降低了产气荚膜梭菌的数量(P<0.01).由此可见,热应激状态下,在基础饲粮中添加甜菜碱可改善AA肉鸡生长性能,提高十二指肠消化酶的活性,维持盲肠内微生物区系平衡,从而减轻热应激对肉鸡的危害.

关键词： 热应激; 肉鸡; 消化酶; 微生物区系

本文引用格式

贺绍君 , 赵书景 , 李静 , 车传燕 , 戴四发 , 刘德义 . 甜菜碱对热应激肉鸡生长性能、十二指肠消化酶活性及盲肠微生物区系的影响[J]. 动物营养学报, 2014 , 26(12) : 3731 -3739 . DOI: 10.3969/j.issn.1006-267x.2014.12.024

Abstract

The study was performed to explore the effects of betaine on growth performance, activities of duodenum digestive enzymes and cecal mircoflora of heat-stressed broilers. A total of 270 14-day-old Arbor Acres (AA) broilers were randomly divided into 6 groups with 3 replicates per group and 15 chicks per replicate. The experimental groups including normal temperature group, heat-stressed group, normal temperature+0.1% betaine group, heat-stressed+0.1% betaine group, heat-stressed+0.2% betaine group, and heat-stressed+0.4% betaine group. Heat stressed broilers were reared in high ambient temperature at (32±1) ℃. The pre-test lasted for 7 d, and formal test lasted for 21 d. The results showed as follows: 1) body weight of heat-stressed broilers aged from 28 to 42 days was significantly increased (P<0.01), and the ratio of feed to gain of heat-stressed broilers aged from 22 to 35 days was significantly decreased by diet supplemented betaine (P<0.01). 2) The activities of trypsinase, amylase and pancrelipase in duodenum of heat-stressed broilers aged 28, 35 and 42 days were significantly increased by betaine with different concentrations (P<0.05 or P<0.01), except trypsinase activity of heat-stressed broilers of 28 days old fed diet supplemented with 0.1% or 0.2% betaine and amylase activity of heat-stressed broilers of 35 days old fed diet supplemented with 0.1% betaine were no significantly difference (P>0.05). 3) Diet supplemented with betaine could significantly increase the numbers of Lactobacillus in cecum (P<0.01), and significantly reduce the numbers of Clostridium perfringens in cecum of heat-stressed broilers (P<0.01). In conclusion, the basal diet supplemented with betaine may improve the growth performance, increase the activities of duodenum digestive enzymes and maintain the stabilization of cecal microflora of heat-stressed AA broilers, then release heat stress hazard to broilers.

Key words： heat stress; broiler; digestive enzyme; microflora

参考文献

[1] LARA L J,ROSTAGNO M H.Impact of heat stress on poultry production[J].Animals,2013,3(2):356-369.  

[2] 郝婧宇,卢庆萍,张宏福,等.持续高温对不同性别北京油鸡脂肪代谢及血液脂肪细胞因子水平的影响[J].动物营养学报,2012,24(6):1038-1043.

[3] YANG P C,HE S H,ZHENG P Y.Investigation into the signal transduction pathway via which heat stress impairs intestinal epithelial barrier function[J].Journal of Gastroenterology and Hepatology,2007,22(11):1823-1831.  

[4] GUO S H,AL-SADI R,YE D M,et al.Endotoxin induced increase in intestinal epithelial tight junction permeability is mediated by toll-like receptor 4 signal transduction pathway[J].Gastroenterology,2011,140(5S):S475-S476.

[5] PEKKINEN J,OLLI K,HUOTARI A,et al.Betaine supplementation causes increase in carnitine metabolites in the muscle and liver of mice fed a high-fat diet as studied by nontargeted LC-MS metabolomics approach[J].Molecular Nutrition & Food Research,2013,57(11):1959-1968.  

[6] SAKOMURA N K,BARBOSA N A A,DA SILVA E P,et al.Effect of betaine supplementation in diets for broiler chickens on thermoneutral environment[J].Revista Brasileira de Ciências Agrárias,2013,8(2):336-341.  

[7] 林海,杜荣.环境温度对肉鸡消化道内食糜排空和消化酶活性的影响[J].动物营养学报,2001,13(1):49-53.

[8] 康磊,李文立,姜建阳,等.谷氨酰胺对热应激肉鸡盲肠微生物区系的影响[J].中国兽医学报,2013,33(1):80-84.

[9] QUINTEIRO-FILHO W M,RIBEIRO A,FERRAZ-DE-PAULA V,et al.Heat stress impairs performance parameters,induces intestinal injury,and decreases macrophage activity in broiler chickens[J].Poultry Science,2010,89(9):1905-1914.  

[10] SINHA R K.Study of changes in some pathophysiological stress markers in different age groups of an animal model of acute and chronic heat stress[J].Iranian Biomedical Journal,2007,11(2):101-111.

[11] FENG Y,YANG X J,WANG Y B,et al.Effects of immune stress on performance parameters,intestinal enzyme activity and mrna expression of intestinal transporters in broiler chickens[J].Asian-Australasian Journal of Animal Sciences,2012,25(5):701-707.  

[12] SAYED M A M,DOWNING J.The effects of water replacement by oral rehydration fluids with or without betaine supplementation on performance,acid-base balance,and water retention of heat-stressed broiler chickens[J].Poultry Science,2011,90(1):157-167.  

[13] HASSAN R A,ATTIA Y A,EL-GANZORY E H.Growth,carcass quality and serum constituents of slow growing chicks as affected by betaine addition to diets containing Ⅰ.Different levels of choline[J].International Journal of Poultry Science,2005,4(11):840-850.  

[14] HAMIDI H,JAHANIAN R,POURREZA J.Effect of dietary betaine on performance,immunocompetence and gut contents osmolarity of broilers challenged with a mixed coccidial infection[J].Asian Journal of Animal and Veterinary Advances,2010,5(3):193-201.  

[15] EKLUND M,MOSENTHIN R,TAFAJ M,et al.Effects of betaine and condensed molasses solubles on nitrogen balance and nutrient digestibility in piglets fed diets deficient in methionine and low in compatible osmolytes[J].Archives of Animal Nutrition,2006,60(4):289-300.  

[16] 安立龙,效梅,黄志毅,等.不同剂量甜菜碱对热应激肉鸡组织器官发育的影响[J].家畜生态学报,2005,26(3):40-45.

[17] CHAMRUSPOLLERT M,PESTI G M,BAKALLI R I.Influence of temperature on the arginine and methionine requirements of young broiler chicks[J].The Journal of Applied Poultry Research,2004,13(4):628-638.  

[18] DUNN A J,ANDO T,BROWN R F,et al.HPA axis activation and neurochemical responses to bacterial translocation from the gastrointestinal tract[J].Annals of the New York Academy of Sciences,2003,992(1):21-29.  

[19] TOROK V A,OPHEL-KELLER K,LOO M,et al.Application of methods for identifying broiler chicken gut bacterial species linked with increased energy metabolism[J].Applied and Environmental Microbiology,2008,74(3):783-791.  

[20] QUINTEIRO-FILHO W M,RODRIGUES M V,RIBEIRO A,et al.Acute heat stress impairs performance parameters and induces mild intestinal enteritis in broiler chickens:role of acute hypothalamic-pituitary-adrenal axis activation[J].Journal of Animal Science,2012,90(6):1986-1994.  

[21] SONG J,XIAO K,KE Y L,et al.Effect of a probiotic mixture on intestinal microflora,morphology,and barrier integrity of broilers subjected to heat stress[J].Poultry Science,2014,93(3):581-588.  
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2