研究简报 Short Communications

油脂类型和水平对肉鸡组织18S rRNA表达的影响

  • 张雯雯 ,
  • 鲁卫卫 ,
  • 王婷 ,
  • 赵艺文 ,
  • 张朋飞 ,
  • 王现勇 ,
  • 陈文 ,
  • 黄艳群
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  • 1. 河南农业大学牧医工程学院, 郑州 450002;
    2. 河南商都生物技术股份有限公司, 郑州 466311

收稿日期: 2014-06-30

  网络出版日期: 2014-12-05

基金资助

国家自然科学基金(1272434);郑州市动物生物技术重点实验室;郑州市创新型科技人才队伍建设工程——科技创新团队项目(121PCXTD516)

Effects of Different Types and Levels of Oil on the Expression of 18S rRNA in Broilers

  • ZHANG Wenwen ,
  • LU Weiwei ,
  • WANG Ting ,
  • ZHAO Yiwen ,
  • ZHANG Pengfei ,
  • WANG Xianyong ,
  • CHEN Wen ,
  • HUANG Yanqun
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  • 1. Animal Husbandry Medical College of Engineering, Henan Agricultural University, Zhengzhou 450002, China;
    2. Shang Du Biological Technology Co., Ltd. of Henan, Zhengzhou 466311, China

Received date: 2014-06-30

  Online published: 2014-12-05

摘要

本试验旨在探究不同类型和水平的油脂对肉鸡肝脏、胸肌中18S rRNA表达的影响.试验选用240只1日龄科宝肉鸡母雏,随机分为8个组(分别在饲粮中添加2.50%、5.00%的亚麻油、玉米油、芝麻油和猪油),每个组6个重复,每个重复5只鸡,于42日龄分别从每个重复随机选取1只屠宰取样.结果表明:1)油脂类型和水平对肉鸡胸肌、肝脏中18S rRNA相对表达量影响极显著(P<0.01),且两者的交互作用对肝脏中18S rRNA相对表达量影响也极显著(P<0.01);2)与2.50%油脂水平相比,除5.00%亚麻油水平对肝脏中18S rRNA相对表达量影响不显著(P>0.05)以外,提高油脂水平(5.00%)可显著或极显著上调肉鸡胸肌、肝脏中18S rRNA相对表达量(P<0.05或P<0.01);3)在肉鸡胸肌、肝脏中,亚麻油组18S rRNA相对表达量不同程度地高于玉米油组、芝麻油组、猪油组(P<0.05或P>0.05).研究表明,肉鸡肝脏和胸肌18S rRNA表达受饲粮添加油脂类型和水平的影响.

本文引用格式

张雯雯 , 鲁卫卫 , 王婷 , 赵艺文 , 张朋飞 , 王现勇 , 陈文 , 黄艳群 . 油脂类型和水平对肉鸡组织18S rRNA表达的影响[J]. 动物营养学报, 2014 , 26(12) : 3814 -3820 . DOI: 10.3969/j.issn.1006-267x.2014.12.035

Abstract

This experiment was conducted to study the effects of different types and levels of oil on the expression of 18S rRNA in liver and chest muscle of broilers. A total of 240 1-day-old female Cobb-500 broilers were fed in this experiment. Broilers were randomly assigned to 8 groups (fed basal diets added with 2.50% and 5.00% linseed oil, corn oil, sesame oil and lard oil, respectively) with 6 replicates per group and 5 broilers per replicate. One broiler of each replicate was selected to slaughter at age of 42 days. The results showed as follows: 1) the relative expression levels of 18S rRNA in liver and breast muscle of broilers were both significantly affected by the oil type and level (P<0.01), and the interactive effect of oil type and level also had significantly effect on the relative expression level of 18S rRNA in liver of broilers (P<0.01). 2) Compared with oil level of 2.50%, except 5.00% linseed level did not significantly affect relative expression level of 18S rRNA in liver of broilers (P>0.05), the increasing of oil level (5.00%) significantly increased the relative expression level of 18S rRNA in liver and breast muscle of broilers (P<0.05 or P<0.01). 3) The relative expression levels of 18S rRNA of linseed oil groups were higher than corn oil, sesame oil and lard oil groups both in liver and breast muscle of broilers at varying degrees (P<0.05 or P>0.05). The results of this study show that the 18S rRNA expression in liver and breast muscle of broilers is affected by both oil type and level.

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