动物营养学报 >

2014 , Vol. 26 >Issue 12: 3821 - 3828

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.12.036

研究简报 Short Communications

饲粮中添加葵花籽油和茶油对泌乳水牛生产性能及乳脂脂肪酸组成的影响

  • 梁贤威 ,
  • 张双双 ,
  • 韦升菊 ,
  • 梁辛 ,
  • 杨承剑 ,
  • 邹彩霞 ,
  • 杨炳壮 ,
  • 梁明振
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  • 1. 中国农业科学院广西水牛研究所, 农业部(广西)水牛遗传繁育重点实验室, 南宁 530002;
    2. 广西大学动物科学技术学院, 南宁 530005

收稿日期: 2014-06-22

  网络出版日期: 2014-12-05

基金资助

广西科学研究与技术开发国际合作课题(桂科合1298014-6);农业部948项目(2011-G26);广西自然基金重点项目(2012GXNSFDA053012)

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Effects of Sunflower Oil and Tea Oil Supplementations on Performance and Fatty Acid Composition of Milk Fat of Lactating Water Buffaloes

  • LIANG Xianwei ,
  • ZHANG Shuangshuang ,
  • WEI Shengju ,
  • LIANG Xin ,
  • YANG Chengjian ,
  • ZOU Caixia ,
  • YANG Bingzhuang ,
  • LIANG Mingzhen
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  • 1. Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture (Guangxi), Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530002, China;
    2. College of Animal Science, Guangxi University, Nanning 530005, China

Received date: 2014-06-22

  Online published: 2014-12-05

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摘要

本文旨在研究饲粮中添加葵花籽油和茶油对泌乳水牛生产性能及乳脂脂肪酸组成的影响.选取32头健康泌乳水牛(平均年龄为6.0岁,平均体重为620 kg),按产奶量(9 kg/d以上)、泌乳期(处于泌乳中期)相近原则随机分为4组,进行为期6周的饲养试验,其中预试期2周,正试期4周.基础饲料由精料和粗料组成.对照组饲喂基础饲粮;在试验组的精料中分别添加4%的葵花籽油(葵花籽油组)、4%的茶油(茶油组)、2%的葵花籽油+2%的茶油(混合组).结果表明:与对照组相比,各试验组的产奶量均显著下降(P<0.05),乳脂率均显著提高(P<0.05),乳脂产量、乳蛋白率和乳糖率则无显著变化(P>0.05).与对照组相比,葵花籽油组、茶油组和混合组的乳总固形物含量分别提高了5.22%、7.95%和6.17%,其中茶油组和混合组与对照组差异显著(P<0.05).与对照组相比,葵花籽油组乳脂中cis9,trans11-C18:2[共轭亚油酸(CLA)]的含量提高了44.54%(P<0.05),混合组乳脂中trans11-C18:1的含量提高了22.68%(P<0.05).葵花籽油组、混合组乳脂中C18:2的含量均显著高于对照组(P<0.05);葵花籽油组、茶油组和混合组的多不饱和脂肪酸(PUFA)/饱和脂肪酸(SFA)均比对照组提高,且葵花籽油组和混合组显著高于对照组(P<0.05),其中混合组的PUFA/SFA最高.各组水牛的血液总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白、葡萄糖、总蛋白、尿素氮含量及谷丙转氨酶、谷草转氨酶活性差异不显著(P>0.05).由此得出,泌乳水牛饲粮中添加精料饲喂量4%的葵花籽油、4%的茶油或2%的葵花籽油+2%的茶油合均可提高乳脂率和乳脂中CLA的含量,但会导致产奶量降低;优化乳脂脂肪酸组成上,以葵花籽油和茶油的混合添加效果较好.

关键词： 泌乳水牛; 茶油; 葵花籽油; 生产性能; 脂肪酸组成

本文引用格式

梁贤威 , 张双双 , 韦升菊 , 梁辛 , 杨承剑 , 邹彩霞 , 杨炳壮 , 梁明振 . 饲粮中添加葵花籽油和茶油对泌乳水牛生产性能及乳脂脂肪酸组成的影响[J]. 动物营养学报, 2014 , 26(12) : 3821 -3828 . DOI: 10.3969/j.issn.1006-267x.2014.12.036

Abstract

This experiment was conducted to study the effects of sunflower oil and tea oil supplementations on performance and fatty acid composition of milk fat of lactating water buffaloes.Thirty-two water buffalo (average age=6 years, average body weight=620 kg) were divided into 4 groups according to their milk yield (>9 kg/d) and lactation period (middle) for feeding trial, and whole feeding trial were 6 weeks included 2 weeks for adaptation and 4 weeks for sampling. The basal diet was composed of forage and concentrate. For control group, the experimental water buffalo feed the basal diet, and for the other 3 experimental groups the diets containing the same forage but concentrates supplemented with 4% sunflower oil (sunflower oil group), 4% tea oil (tea oil group), and 2% sunflower oil+2% tea oil (mixed group), respectively. The results showed as follows: compared with the control group, the milk yield in the experimental groups was significantly decreased (P<0.05), while the milk fat percentage was significantly increased (P<0.05). For milk fat yield, milk protein percentage and lactose percentage, there were no significant differences among all groups (P>0.05). Compared with the control group, the milk total solid content in the sunflower oil, tea oil and mixed groups was increased by 5.22%, 7.95% and 6.17%, respectively, and there was a significant difference between the control group and the tea oil and mixed groups (P<0.05). Compared with the control group, the cis9, trans11-C18:2 (conjugated linoleic acid, CLA) content of milk fat in the sunflower oil group was increased by 44.54% (P<0.05), and the trans11-C18:1 content of milk fat in the mixed group was increased by 22.68% (P<0.05). Compared with the control group, the C18:2 content of milk fat in the sunflower oil and mixed groups was significantly increased (P<0.05). Compared with the control group, polyunsaturated fatty acid (PUFA)/saturated fatty acid (SFA) in the sunflower oil, tea oil and mixed groups was increased, and there was a significant difference between the control group and the sunflower oil and mixed groups (P<0.05). The highest PUFA/SFA was found in the mixed group. There were no significant differences in the contents of total cholesterol (TCHO), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL-C), glucose (GLU), total protein (TP), urea nitrogen (UN) and the activities of aspartate aminotransferase (ALT), aspartate aminotransferase (AST) in blood among all groups (P>0.05). In conclusion, for water buffaloes, concentrate supplemented with 4% sunflower oil, 4% tea oil or 2% sunflower oil+2% tea oil can increase the milk fat percentage and milk fat CLA content, but decrease the milk yield. For the better fatty acid composition in buffalo milk fat, supplemented with 2% sunflower oil+2% tea oil has the better effect.

Key words： lactating water buffalo; tea oil; sunflower oil; performance; fatty acid composition

参考文献

[1] BELURY M A.Dietary conjugated linoleic acid in health:physiological effects and mechanisms of action[J].Annual Review of Nutrition,2002,22:505-531.

[2] KHANAL R C,OLSON K C.Factors affecting conjugated linoleic acid (CLA) content in milk,meat,and egg:a review[J].Pakistan Journal of Nutrition,2004,3(2):82-98.  

[3] KELLY M L,KOLVER E S,BAUMAN D E,et al.Effect of intake of pasture on concentrations of conjugated linoleic acid in milk of lactating cows[J].Journal of Dairy Science,1998,81(6):1630-1636.  

[4] 薛秀恒,王菊花,王志耕.饲料调控对奶牛共轭亚油酸合成的影响[J].饲料博览,2004(3):28-31.

[5] GRIINARI J M,CORL B A,LACY S H,et al.Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by delta(9)-desaturase[J].The Journal of Nutrition,2000,130(9):2285-2291.

[6] DHIMAN T R,SATTER L D,PARIZA M W,et al.Conjugated linoleic acid (CLA) content of milk from cows offered diets rich in linoleic and linolenic acid[J].Journal of Dairy Science,2000,83(5):1016-1027.  

[7] MÉNARD O,AHMAD S,ROUSSEAU F,et al.Buffalo vs.cow milk fat globules:size distribution,zeta-potential,compositions in total fatty acids and in polar lipids from the milk fat globule membrane[J].Food Chemistry,2010,120(2):544-551.  

[8] 韦升菊,梁贤威,卢雪芬,等.水牛奶和乳牛奶常规乳成分及乳脂脂肪酸组成的初步比较研究[J].畜牧与兽医,2011,43(10):41-43.

[9] 梁贤威,韦升菊,卢雪芬,等.半胱胺对水牛乳脂脂肪酸组成的影响[J].畜牧与兽医,2012,44(8):40-42.

[10] 杨炳壮,卢雪芬,韦升菊,等.饲喂花生油对水牛乳脂脂肪酸组成的影响[J].饲料工业,2012,33(9):40-42.

[11] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2003.

[12] 王小静,沈向真,韩航如,等.毛细管气相色谱法测定乳脂中的cis-9,trans-11-共轭亚油酸[J].色谱,2006,24(6):645-647.

[13] 王永,段现来,刘建兵,等.脂肪在奶牛生产中的应用[J].兽药与饲料添加剂,2006,11(6):24-26.

[14] 金曙光,周玉财,陈润易,等.添加不同种类棕榈酸产品对奶牛生产性能和血液指标的影响[J].饲料工业,2010(Z2):68-71.

[15] 刘子睿,王佳丽.日粮中添加油脂对于奶牛产奶量及乳脂率的影响[J].今日畜牧兽医,2007(4):6-7.

[16] 薛秀恒,王菊花,王志耕,等.日粮添加葵花籽油与豆油对奶牛生产性能及牛奶中共轭亚油酸含量的影响[J].粮食与饲料工业,2010(4):52-55,63.

[17] SOLOMON R,CHASE L E,BEN-GHEDALIA D,et al.The effect of nonstructural carbohydrate and addition of full fat extruded soybeans on the concentration of conjugated linoleic acid in the milk fat of dairy cows[J].Journal of Dairy Science,2000,83(6):1322-1329.  

[18] WARD A T,WITTENBERG K M,PRZYBYLSKI R.Bovine milk fatty acid profiles produced by feeding dietscontaining solin,flax and canola[J].Journal of Dairy Science,2002,85(5):1191-1196.  

[19] 李鹏,兰伯毅.提高奶牛乳脂率的措施和途径[J].畜牧与饲料科学,2012,33(2):63-65.

[20] GÓMEZ-CORTÉS P,TORAL P G,FRUTOS P,et al.Effect of the supplementation of dairy sheep diet with incremental amounts of sunflower oil on animal performance and milk fatty acid profile[J].Food Chemistry,2011,125(2):644-651.  

[21] KATHIRVELAN C,TYAGI A K.Conjugated linoleic acid content of milk from buffaloes fed a mustard oil-based diet[J].International Journal of Dairy Technology,2009,62(2):141-146.  

[22] LUCY M C,STAPLES C R,THATCHER W W,et al.Influence of diet composition,dry-matter intake,milk production and energy balance on time of post-partum ovulation and fertility in dairy cows[J].Animal Production,1992,54(3):323-331.  

[23] 王丽芳.奶牛日粮中添加整粒胡麻籽与棉籽对牛奶品质的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2005.

[24] 李春,刘丽波,张兰威.共轭亚油酸生理功能及其应用[J].粮食与油脂,2004(7):15-17.

[25] TYAGI A K,KEWALRAMANI N,DHIMAN T R,et al.Enhancement of the conjugated linoleic acid content of buffalo milk and milk products through green fodder feeding[J].Animal Feed Science and Technology,2007,133(3/4):351-358.

[26] ALLRED S L,DHIMAN T R,BRENNAND C P,et al.Milk and cheese from cows fed calcium salts of palm and fish oil alone or in combination with soybean products[J].Journal of Dairy Science,2006,89(1):234-248.  

[27] KITESSA S M,GULATI S K,ASHES J R,et al.Utilisation of fish oil in ruminants:Ⅱ.Transfer of fish oil fatty acids into goats' milk[J].Animal Feed Science and Technology,2001,89(3/4):201-208.

[28] BELL J A,GRIINARI J M,KENNELLY J J.Effect of safflower oil,flaxseed oil,monensin,and vitamin E on concentration of conjugated linoleic acid in bovine milk fat[J].Journal of Dairy Science,2006,89(2):733-748.  

[29] JOHNSON K A,KINCAID R L,WESTBERG H H,et al.The effect of oilseeds in diets of lactating cows on milk production and methane emissions[J].Journal of Dairy Science,2002,85(6):1509-1515.  

[30] 刘瑞芳.日粮中添加植物油对奶牛瘤胃发酵和乳脂中共轭亚油酸含量影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2006.

[31] 卜登攀,王加启,DHIMAN T R,等.植物油来源亚油酸和亚麻酸对乳脂CLA合成的影响[J].畜牧兽医学报,2007,38(7):663-671.

[32] 刘仕军,卜登攀,王加启,等.日粮添加LA和DHA对乳脂脂肪酸含量及比值的影响[J].动物营养学报,2008,20(5):515-521.
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