野生虎斑乌贼不同组织营养成分分析及评价

高晓兰; 蒋霞敏; 乐可鑫; 汪元; 胡晓鹏; 丰迅

doi:10.3969/j.issn.1006-267x.2014.12.041

动物营养学报 >

2014 , Vol. 26 >Issue 12: 3858 - 3867

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.12.041

研究简报 Short Communications

野生虎斑乌贼不同组织营养成分分析及评价

高晓兰 ,
蒋霞敏 ,
乐可鑫 ,
汪元 ,
胡晓鹏 ,
丰迅

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宁波大学海洋学院, 宁波 315211

收稿日期: 2014-06-30

网络出版日期: 2014-12-05

基金资助

国家自然科学基金项目(41206114);宁波市农业科技专项(201401C1111001);宁波市科技重点项目(2011C11002)

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Analysis and Evaluation of Nutritional Components in Different Tissues of Wild Sepia pharaonis

GAO Xiaolan ,
JIANG Xiamin ,
LE Kexin ,
WANG Yuan ,
HU Xiaopeng ,
FENG Xun

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College of Ocean, Ningbo University, Ningbo 315211, China

Received date: 2014-06-30

Online published: 2014-12-05

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摘要

本试验对野生虎斑乌贼5种组织(肌肉、肝脏、卵巢、缠卵腺、副缠卵腺)的营养成分进行分析,旨在对其营养价值进行评估.随机选取雌、雄野生虎斑乌贼[雌性个体平均体长(31.37±0.32) cm,平均体重(2 173.33±141.10) g;雄性个体平均体长(30.97±0.74) cm,平均体重(2 476.33±117.54) g]各10尾,采用生化分析手段对其肌肉、肝脏、卵巢、缠卵腺、副缠卵腺中水分、粗灰分、粗蛋白质、粗脂肪含量以及氨基酸、脂肪酸组成与含量进行测定与分析,每个组织测定3个平行.结果表明:野生虎斑乌贼5种组织中,水分含量为60.12%~78.33%,各组织间差异显著(P<0.05);粗蛋白质含量为31.47%~77.93%,表现为肌肉(77.93%)>卵巢(62.39%)>副缠卵腺(58.62%)>缠卵腺(57.64%)>肝脏(31.47%),各组织间差异显著(P<0.05);粗灰分含量为1.26%~3.18%,除肌肉与缠卵腺无显著差异(P>0.05)外,其他组织间差异显著(P<0.05),以副缠卵腺最高,肝脏最低;粗脂肪含量为2.46%~12.82%,表现为肝脏(12.82%)>缠卵腺(4.80%)>副缠卵腺(3.42%)>肌肉(3.14%)>卵巢(2.46%),除肌肉与副缠卵腺无显著差异(P>0.05)外,其他组织间差异显著(P<0.05).野生虎斑乌贼5种组织均含有17种氨基酸,其中总氨基酸(TAA)含量为27.68%~77.87%,必需氨基酸(EAA)含量为13.60%~31.80%;共检出26种脂肪酸,包括12种饱和脂肪酸(SFA),7种单不饱和脂肪酸(MUFA)和7种多不饱和脂肪酸(PUFA),其中PUFA含量为27.48%~54.15%,以肝脏最低,显著低于其他组织(P<0.05),具体为缠卵腺(54.15%)>肌肉(47.74%)>卵巢(45.50%)>副缠卵腺(41.49%)>肝脏(27.48%);C20:5n-3(EPA)含量为6.72%~16.03%,C22:6n-3(DHA)含量为12.79%~32.20%.由此得出,野生虎斑乌贼是一种高蛋白质、低脂肪、氨基酸含量高且种类齐全、PUFA含量丰富(尤其是富含EPA和DHA)、营养价值较高的头足类.

关键词： 野生虎斑乌贼; 组织; 营养成分; 评价

本文引用格式

高晓兰 , 蒋霞敏 , 乐可鑫 , 汪元 , 胡晓鹏 , 丰迅 . 野生虎斑乌贼不同组织营养成分分析及评价[J]. 动物营养学报, 2014 , 26(12) : 3858 -3867 . DOI: 10.3969/j.issn.1006-267x.2014.12.041

Abstract

In order to evaluate the nutritive value of wild Sepia pharaonis, the nutritional components of different tissues (muscle, liver, ovary, nidamental gland and accessory nidamental gland) were determined. Ten female and 10 male wild Sepia pharaonis were randomly selected [female individual: average body length (31.37±0.32) cm, average body weight (2 173.33±141.10) g; male individual: average body length (30.97±0.74) cm, average body weight (2 476.33±117.54) g]. The contents of moisture, ash, crude protein, crude fat, and the composition and contents of amino acids and fatty acids in muscle, liver, ovary, nidamental gland and accessory nidamental gland were determined by biochemical analysis methods. Each organization was measured with 3 replications. The results showed that moisture and crude protein contents were significantly different among different tissues (P<0.05). The moisture content in 5 tissues of wild Sepia pharaonis was 60.12% to 78.33%. The crude protein content in 5 tissues of wild Sepia pharaonis was 31.47% to 77.93%, with the highest value in muscle (77.93%), followed by ovary (62.39%), accessory nidamental gland (58.62%), nidamental gland (57.64%) and liver (31.47%). The ash content in 5 tissues of wild Sepia pharaonis was 1.26% to 3.18%, and there was a significant difference (P<0.05) with exception of muscle and nidamental gland (P>0.05), with the highest value in accessory nidamental gland and the lowest value in liver. The crude fat content in 5 tissues of wild Sepia pharaonis was 2.46% to 12.82%, and there was a significant difference (P<0.05) with exception of muscle and accessory nidamental gland (P>0.05). The liver had the highest crude fat content (12.82%), followed by nidamental gland (4.80%), accessory nidamental gland (3.42%), muscle (3.14%) and ovary (2.46%). A total of 17 amino acids were identified in 5 tissues of wild Sepia pharaonis. The total amino acid content in 5 tissues of wild Sepia pharaonis was 27.68% to 77.87%, and the essential amino acid content was 13.60% to 31.80%. A total of 26 fatty acids were found, including 12 saturated fatty acids (SFA), 7 mono-unsaturated fatty acids (MUFA) and 7 polyunsaturated fatty acids (PUFA). The PUFA content was 27.48% to 54.15% of total fatty acids, and there was a significant difference between liver and other tissues (P<0.05), decreasing as nidamenta gland (54.15%), muscle (47.74%), ovary (45.50%), accessory nidamental gland (41.49%) and liver (27.48%). The contents of C20:5n-3 (EPA) and C22:6n-3 (DHA) were 6.72% to 16.03% and 12.79% to 32.20%, respectively. In all, wild Sepia pharaonis is a kind of nutrition rich cephalopods with high content of protein, low content of fat, various kinds and high contents of amino acids, and lots of PUFA, especially EPA and DHA.

Key words： wild Sepia pharaonis; tissue; nutritional components; evaluation

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