动物营养学报 >

2015 , Vol. 27 >Issue 1: 59 - 66

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.01.009

猪营养 Swine Nutrition

丙氨酰-谷氨酰胺对断奶仔猪小肠黏膜固有层免疫球蛋白A浆细胞数量、分泌型免疫球蛋白A及黏膜中白细胞介素含量的影响

  • 叶亚玲 ,
  • 王自蕊 ,
  • 游金明 ,
  • 邓宸玺 ,
  • 辛向荣
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  • 江西农业大学江西省动物营养重点实验室, 南昌 330045

收稿日期: 2014-07-18

  网络出版日期: 2015-01-10

基金资助

国家自然科学基金(31360554);江西省青年科学家培养计划项目(2011BCB23008);江西省教育厅落地计划项目(KJLD14027)

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Effects of Alanyl-Glutamine on the Number of Immunoglobulin A Plasma Cells in Intestinal Lamina Propria and the Contents of Secretory Immunoglobulin A and Interleukin in Mucosal of Weaner Piglets

  • YE Yaling ,
  • WANG Zirui ,
  • YOU Jinming ,
  • DENG Chenxi ,
  • XIN Xiangrong
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  • Key Lab of Animal Science of Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2014-07-18

  Online published: 2015-01-10

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摘要

本试验旨在研究丙氨酰-谷氨酰胺(Ala-Gln)对断奶仔猪小肠黏膜固有层免疫球蛋白A(IgA)浆细胞数量、分泌型免疫球蛋白A(SIgA)及黏膜中白细胞介素(IL)含量的影响。试验选用(21±2)日龄断奶、初始体重(6.7±0.9) kg的长白×大白去势仔猪100头,按完全随机区组法分为4组,每组5个重复,每重复5头仔猪。对照组饲喂基础饲粮,试验组分别饲喂基础饲粮中添加0.15%、0.30%和0.45% Ala-Gln的试验饲粮。试验期21 d。结果显示:1)与对照组相比,饲粮中添加0.15%~0.45% Ala-Gln均显著提高仔猪断奶后第7、14、21天十二指肠以及空肠黏膜固有层IgA浆细胞数量(P<0.05),特别是0.30% Ala-Gln组仔猪在断奶第14天空肠IgA浆细胞数比对照组增加22.6%(P<0.05);在回肠中,0.30% Ala-Gln主显著提高断奶后第7、14天仔猪IgA浆细胞数(P<0.05)。2)断奶仔猪空肠黏膜SIgA含量随着Ala-Gln添加量呈二次曲线增长规律。在断奶后第7天,0.30% Ala-Gln组仔猪SIgA含量与对照组相比极显著提高了70.4%(P<0.01);在断奶后第14、21天,0.15%、0.30%Ala-Gln与对照组相比显著提高了SIgA含量(P<0.05)。3)与对照组相比,饲粮中添加0.15%、0.30% Ala-Gln可显著提高仔猪早期断奶后第7天黏膜IL-2、IL-5含量,分别显著提高断奶后第14天IL-5、IL-2含量(P<0.05),其他添加水平无显著影响(P>0.05);随着饲粮中Ala-Gln添加量的增加,各组IL-6、IL-10的含量呈线性增长趋势,且显著高于对照组(P<0.05)。综上所述,饲粮中添加Ala-Gln可提高肠黏膜固有层IgA浆细胞数量、黏膜SIgA分泌量及IL含量,提高断奶仔猪肠道黏膜细胞免疫。

关键词： Ala-Gln; 肠道黏膜; 免疫; 断奶仔猪

本文引用格式

叶亚玲 , 王自蕊 , 游金明 , 邓宸玺 , 辛向荣 . 丙氨酰-谷氨酰胺对断奶仔猪小肠黏膜固有层免疫球蛋白A浆细胞数量、分泌型免疫球蛋白A及黏膜中白细胞介素含量的影响[J]. 动物营养学报, 2015 , 27(1) : 59 -66 . DOI: 10.3969/j.issn.1006-267x.2015.01.009

Abstract

The study was conducted to study the effects of alanyl-glutamine (Ala-Gln) on the number of Immunoglobulin A (IgA) plasma cells in intestinal lamina propria and the contents of secretory immunoglobulin A (SIgA) and interleukin (IL) in mucosal of weaner piglets. The experiment used a randomized complete block design, a total of 100 castrated weaner piglets (Landrace ×Large White ) at (21±2) days of age with initial body weight of (6.7±0.9) kg were assigned to four groups with five replicates in each group and five piglets in each replicate. Piglets in the control group were fed a basal diet (free alanyl-glutamine), and the others were fed the basal diet supplemented with 0.15%, 0.30% and 0.45% Ala-Gln, respectively. The experiment lasted for 21 days. The results showed as follows: 1) compared with the control group, dietary supplemented with 0.15% to 0.45% Ala-Gln significantly elevated the number of IgA plasma cells in duodenum and jejunum lamina propria on 7, 14 and 21 days of post-weaning (P<0.05), especially, the number of IgA plasma cells in jejunum of 0.30% Ala-Gln group was increased by 22.6% on 14 days of post-weaning (P<0.05); in ileum, the number of IgA plasma cells of 0.30% Ala-Gln group was significantly increased on 7 and 14 days of post-weaning (P<0.05). 2) With the increasing of Ala-Gln addition in diet, the content of SIgA in jejunal mucosal quadratically increased. Compared with the control group, the SIgA content of 0.30% Ala-Gln group was increased by 70.4% on 7 days of post-weaning (P<0.01), both 0.15% and 0.30% Ala-Gln groups was significantly increased on 14 and 21 days of post-weaning (P<0.05). 3) Compared with the control group, dietary supplemented with 0.15% and 0.30% Ala-Gln significantly increased the contents of IL-2 and IL-5 on on 7 days of post-weaning (P<0.05), and also significantly increased the contents of IL-5, IL-2 on 14 days of post-weaning (P<0.05), respectively. The other Ala-Gln addition had no significantly difference (P>0.05). With the increasing of Ala-Gln addition in diet, the contents of IL-6, IL-10 showed a linear increasing trend, and was markedly higher than the control group (P<0.05). In conclusion, dietary supplemented with Ala-Gln can elevate the number of IgA plasma cells and the content of SIgA and interleukin in intestinal mucosa, and improve the intestinal mucosa cellular immunity of weaner piglets.

Key words： Ala-Gln; intestinal mucosa; immunity; weaner piglets

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