T-2毒素对BALB/c小鼠生长性能、血液指标和抗氧化能力的影响

陈慧英; 杨俊花; 罗爱琼; 郭文博; 韩薇; 赵志辉

doi:10.3969/j.issn.1006-267x.2015.01.026

动物营养学报 >

2015 , Vol. 27 >Issue 1: 212 - 220

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.01.026

饲料安全 Feed Security

T-2毒素对BALB/c小鼠生长性能、血液指标和抗氧化能力的影响

陈慧英 ,
杨俊花 ,
罗爱琼 ,
郭文博 ,
韩薇 ,
赵志辉

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1. 上海海洋大学食品学院, 上海 201306;
2. 上海市农业科学院农产品质量标准与检测技术研究所, 上海 201403

收稿日期: 2014-07-24

网络出版日期: 2015-01-10

基金资助

真菌毒素对畜禽产品质量安全风险评估关键技术研究[沪农科攻字(2013)第3~8号];食用农产品非法添加物及残留检测技术标准研究(12DZ0502800);热带水果电子束辐照加工关键技术及食品安全相关技术标准研究(11DZ0502800)

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Effects of T-2 Toxin on Growth Performance, Blood Indexes and Antioxidant Ability in BALB/c Mice

CHEN Huiying ,
YANG Junhua ,
LUO Aiqiong ,
GUO Wenbo ,
HAN Wei ,
ZHAO Zhihui

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1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
2. Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Received date: 2014-07-24

Online published: 2015-01-10

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摘要

本试验旨在研究T-2毒素对BALB/c小鼠生长性能、血常规指标、血清生化指标和肝脏抗氧化能力的影响。选用平均体重约为20 g的BALB/c小鼠80只,随机分成4个组,每个组20个重复,每个重复1只小鼠。4个组分别为对照组和3个不同T-2毒素剂量组,即0.4、1.0和2.5 mg/kg BW组,连续灌服28 d。结果表明,2.5 mg/kg BW组小鼠死亡率达25%,平均日增重和平均日采食量极显著降低(P<0.01),心脏、肝脏、脾脏、肺脏、肾脏和大脑的脏器系数显著或极显著增加(P<0.05或P<0.01),睾丸、精囊腺、子宫和卵巢等生殖系统脏器系数显著或极显著降低(P<0.05或P<0.01)。与对照组相比,0.4和1.0 mg/kg BW组肝脏脏器系数极显著增加(P<0.01),白细胞(WBC)、淋巴细胞(LY)、单核细胞(MO)、中性粒细胞(GR)数量均显著或极显著增高(P<0.05或P<0.01)。与对照组相比,2.5 mg/kg BW组MO、GR、血小板(PLT)数量极显著增高(P<0.01),但红细胞数量(RBC)和血红蛋白(HGB)含量显著或极显著降低(P<0.05或P<0.01)。血清生化指标显示,随T-2毒素剂量的增加,葡萄糖(GLU)、尿素(urea)、肌酐(CRE)含量和碱性磷酸酶(ALP)、丙氨酸氨基转移酶(ALT)活性呈增加趋势,但甘油三酯(TG)和白蛋白(ALB)含量呈降低趋势,与对照组相比均差异显著或极显著(P<0.05或P<0.01)。随T-2毒素剂量的增加,小鼠肝脏组织中丙二醛(MDA)含量呈增加趋势,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性呈降低趋势。由此可知,T-2毒素剂量在低于1.0 mg/kg BW时对BALB/c小鼠具有免疫刺激作用,高剂量的T-2毒素抑制动物生长性能、引起肝脏和肾脏组织损伤、对肝脏的抗氧化能力也具有显著的抑制作用,呈剂量效应关系。

关键词： T-2毒素; 生长性能; 血常规指标; 血清生化指标; 抗氧化

本文引用格式

陈慧英 , 杨俊花 , 罗爱琼 , 郭文博 , 韩薇 , 赵志辉 . T-2毒素对BALB/c小鼠生长性能、血液指标和抗氧化能力的影响[J]. 动物营养学报, 2015 , 27(1) : 212 -220 . DOI: 10.3969/j.issn.1006-267x.2015.01.026

Abstract

This study was conducted to investigate the effects of T-2 toxin on growth performance, blood routine examination, serum biochemical indexes and antioxidant ability of liver in mice. A total of 80 BALB/c mice weighted about 20 g were randomly allotted to 4 groups with 20 replicates per group and 1 mouse per replicate. The mice were fed one of the 4 kinds of diets [0 (control), 0.4, 1.0, 2.5 mg/kg BW T-2 toxin exposed daily via intragastric administration] for 28 days. The results showed as follows: in 2.5 mg/kg BW group, the morality of mice was close to 25%, and average daily gain and average daily feed intake were significantly decreased (P<0.01). However, the organ coefficients of heart, liver, spleen, lung, kidney and brain in 2.5 mg/kg BW group were increased markedly (P<0.05 or P<0.01), but those of testis, seminal vesicle, uterus and ovaries were reduced significantly (P<0.05 or P<0.01). Compared with control group, organ coefficient of liver and the counts of white blood cell (WBC), lymphocyte (LY), monocytes (MO), neutrophil granulocyte (GR) were significantly increased in 0.4 and 1.0 mg/kg BW groups (P<0.05 or P<0.01), respectively. Additionally, compared with control group, the counts of MO, GR and platelet (PLT) in 2.5 mg/kg BW group were significantly increased (P<0.01), however, red blood count (RBC) and hemoglobin (HGB) content were significantly reduced (P<0.05 or P<0.01), respectively. Furthermore, in serum biochemical indexes, the contents of glucose (GLU), urea and creatinine (CRE), and the activities of alkaline phosphatase (ALP) and alanine aminotransferase (ALT) had improved trends with the increase of T-2 toxin dose, but the contents of triglyceride (TG) and albumin (ALB) had reduced trends, and those were significantly different with those of control group (P<0.05 or P<0.01). In addition, with the increase of T-2 toxin dose, the content of malondialdehyde (MDA) in liver tissue was also advanced, then the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) had decreased trends. It is concluded that the supplementation of T-2 toxin under 1.0 mg/kg BW can induce the immunostimulation. Nevertheless, the excessive dose can cause the inhibition of growth performance, induce organ damage of liver and kidney, and reduce the antioxidant ability of liver significantly with dose-effect relationship.

Key words： T-2 toxin; growth performance; blood routine examination; serum biochemical indexes; antioxidant

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