鹅血红素对患缺铁性贫血症幼龄大鼠生长发育、铁代谢和器官组织元素分布的影响

解超, 王宝维, 葛文华, 张名爱, 徐晓波, 岳斌

doi:10.3969/j.issn.1006-267x.2015.02.011

动物营养学报 >

2015 , Vol. 27 >Issue 2: 408 - 417

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.02.011

禽营养 Poultry Nutrition

鹅血红素对患缺铁性贫血症幼龄大鼠生长发育、铁代谢和器官组织元素分布的影响

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青岛农业大学优质水禽研究所, 国家水禽产业技术体系营养与饲料功能研究室, 青岛 266109

解超(1989-), 女, 山东滨州人, 硕士研究生, 研究方向为营养与保健.E-mail:xiechaomm@126.com

收稿日期: 2014-09-22

网络出版日期: 2015-02-09

基金资助

国家水禽产业技术体系专项基金(CARS-43-11);山东省农业重大应用技术创新项目(6681301)

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Effects of Goose Heme on Growth Development, Iron Metabolism and Organic Tissues Element Distribution of Iron Deficiency Anemia Young Rats

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Nutrition and Feed Laboratory of China Agriculture Research System Qingdao, Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2014-09-22

Online published: 2015-02-09

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摘要

本试验旨在研究不同水平鹅血红素对患缺铁性贫血症(IDA)3~7周龄大鼠生长发育、铁代谢及其器官组织元素(铁、铜、锰、锌、硒)分布的干预修复作用,以确定鹅血红素的使用效果和适宜补充量.选取同期受孕母鼠开始造模,模型组孕鼠受孕15 d后,开始饲喂低铁饲粮,持续至哺乳期结束(分娩后21 d),IDA模型建成后选取3周龄大鼠180只,随机分为5组(每组6个重复,每个重复6只),即:缺铁组、FeSO₄组、低剂量组、中剂量组、高剂量组,各组分别每天灌注去离子水10 mL/kg BW,1% FeSO₄ 10 mL/kg BW和鹅血红素0.11、0.22、0.33 g/kg BW,均饲喂低铁饲粮.正常组自孕期开始一直饲喂基础标准对照饲粮.试验期28 d.结果表明:1)高剂量组幼龄大鼠的平均日增重、末重、器官指数(除心脏指数)与正常组无显差异著(P>0.05).与缺铁组相比较,高剂量组幼龄大鼠血液中血红蛋白含量、红细胞数、红细胞压积、平均红细胞体积、血清铁、转铁蛋白饱和度显著或极显著升高(P<0.05或P<0.01),红细胞分布宽度和总铁结合力显著或极显著降低(P<0.05或P<0.01),大鼠心脏铁、铜、锌含量显著升高(P<0.05),肝脏铜、锰含量显著或极显著降低(P<0.05或P<0.01).2)FeSO₄溶液作为补铁剂长期服用对幼龄大鼠缺铁模型的干预效果不明显.由此可见,鹅血红素摄入量为0.33 g/kg BW时能有效干预IDA幼龄大鼠的生长发育和造血能力,并能调控幼龄大鼠各器官组织内的元素分布趋于正常,效果优于FeSO₄.

关键词： 鹅血红素; 幼龄大鼠; 生长发育; 血液指标; 元素分布

本文引用格式

解超, 王宝维, 葛文华, 张名爱, 徐晓波, 岳斌 . 鹅血红素对患缺铁性贫血症幼龄大鼠生长发育、铁代谢和器官组织元素分布的影响[J]. 动物营养学报, 2015 , 27(2) : 408 -417 . DOI: 10.3969/j.issn.1006-267x.2015.02.011

Abstract

The experiment was conducted to study the effects of different dosages of goose heme on growth development, iron metabolism and organic tissues element (iron, copper, manganese, zinc and selenium) distribution of iron deficiency anemia (IDA) rats aged from 3 to 7 weeks, and to find the appropriate supplemental level of goose heme and its feeding effect. The same late pregnancy period maternal were selected and fed by low-iron diet, from 15 days after pregnancy to the end of lactation, to establish the IDA model. After that, a total of 180 three-week-old young rats were randomly assigned into 5 groups with 6 replicates per group and 6 rats per replicate. Adding distilled water 10 mL/kg BW (iron deficiency group), 1% FeSO₄ 10 mL/kg BW (FeSO₄ group) and goose heme 0.11 (low-dose group), 0.22 (mid-dose group), 0.33 g/kg BW (high-dose group), fed low iron feed. Normal group had been fed a basal control diet during modling and intervention period. The experiment lasted for 4 weeks. The results showed as follows: 1) the average body gain, final weight and organ indexes (except heart index) in high-dose group had no significant different with normal group (P>0.05). Compared with the iron deficiency group, the hemoglobin, red blood cell, hematokrit, mean corpuscular volume, serum iron and transferrin saturation in blood of young rats in high-dose group were significantly increased (P<0.05 or P<0.01), the erythrocyte hemoglobin distribution width and total iron binding capacity in high-dose group were significantly decreased (P<0.05 or P<0.01), the contents of iron, copper and zinc in heart of rats in high-dose group were significantly increased (P<0.05), the contents of copper and manganese in high-dose group were significantly decreased (P<0.05 or P<0.01). 2)Taking FeSO₄ as iron supplement to intervene IDA model was not significant after long-term use. In conclusion, supplemented with 0.33 g/kg BW goose heme can effective intervene the growth development and hematopoietic ability of IDA young rats, and to make organic tissues element distribution tends to be normal, the effects are better than FeSO₄.

Key words： goose heme; young rats; growth development; blood parameters; element distribution

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