动物营养学报 >

2015 , Vol. 27 >Issue 2: 418 - 425

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.02.012

反刍动物营养 Ruminant Nutrition

亚急性瘤胃酸中毒对奶山羊血浆细胞因子和激素含量的影响

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  • 1. 内蒙古农牧业科学院动物营养与饲料研究所, 呼和浩特 010031;
    2. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
    3. 重庆市畜牧科学院动物营养研究所, 重庆 402460
胡红莲(1977-), 女, 内蒙古乌拉特前旗人, 副研究员, 博士, 主要从事反刍动物营养调控技术研究.E-mail:honglianhu2010@163.com

收稿日期: 2014-09-02

  网络出版日期: 2015-02-09

基金资助

国家自然科学基金项目(31101739);现代农业(奶牛)产业技术体系建设专项资金(CARS-37)

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Effects of Subacute Ruminal Acidosis on Plasma Cytokine and Hormone Contents in Dairy Goats

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  • 1. Institue of Animal Nutrition and Feed, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
    2. College of Animal Science, Inner Mongolia Agriculture University, Hohhot 010018, China;
    3. Institute of Animal Nutrition, Chongqing Academy of Animal Sciences, Chongqing 402460, China

Received date: 2014-09-02

  Online published: 2015-02-09

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摘要

本试验旨在研究以递增饲粮非纤维性碳水化合物与中性洗涤纤维比(NFC/NDF)方式诱导奶山羊发生亚急性瘤胃酸中毒(SARA)后,血浆细胞因子和激素含量的变化.选取9只体况良好,体重接近的泌乳期莎能奶山羊,随机分为3组(对照组、SARA组、恢复组,n=3),对照组饲喂基础饲粮,SARA组和恢复组先后饲喂NFC/NDF为1.40、1.79、2.31、3.23的4种试验饲粮诱导SARA发生,每种饲喂15 d,恢复组奶山羊诱导SARA后自由采食青干草30 d.对照组3只羊分别在饲养30、60(与SARA组同时)和90 d(与恢复组同时)屠宰.结果表明:1)SARA长期提高了血浆LPS浓度,恢复组与SARA组差异不显著(P>0.05).2)与对照组相比,SARA组奶山羊血浆中促炎性细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β和IL-6含量呈升高趋势(P>0.05),IL-2含量显著升高(P<0.05),IL-8含量呈降低趋势(P>0.05),γ-干扰素(IFN-γ)含量显著降低(P<0.05),抗炎性细胞因子IL-4和IL-10含量均呈降低趋势(P>0.05);SARA组奶山羊血浆中生长激素、胰岛素样生长因子-Ⅰ、胰岛素含量变化不显著(P>0.05),催乳素(PRL)和皮质醇(COR)含量均显著升高(P<0.05).3)与SARA组相比,恢复组奶山羊血浆中TNF-α、IL-1β、IL-2、IL-6这些促炎性细胞因子的含量仍持续性升高,其中IL-1β和IL-2含量显著升高(P<0.05);恢复组奶山羊血浆中PRL含量仍维持与SARA组同一水平(P>0.05),而COR含量则回归到对照组水平(P>0.05).本研究结果显示,SARA可引起奶山羊血浆中长时间含有一定量的LPS,引发机体免疫和炎症反应,并引起内分泌激素的变化,这一系列变化导致奶山羊长期处于应激状态.

关键词： 亚急性瘤胃酸中毒; 奶山羊; 细胞因子; 激素

本文引用格式

胡红莲, 谢天宇, 杨淑青, 高民, 姚焰础 . 亚急性瘤胃酸中毒对奶山羊血浆细胞因子和激素含量的影响[J]. 动物营养学报, 2015 , 27(2) : 418 -425 . DOI: 10.3969/j.issn.1006-267x.2015.02.012

Abstract

This experiment was conducted to investigate the effects of subacute ruminal acidosis (SARA) induced by gradually increasing dietary non-fiber carbohydrate/neutral detergent fiber ratios (NFC/NDF) on plasma hormone and cytokine contents in dairy goats. Nine healthy lactating Saanen dairy goats with similar body weight were randomly allocated to three groups (control group, SARA group and recovery group, n=3). Dairy goats in control group were fed a basal diet; those in SARA group and recovery group were fed diets with different NFC/NDF (1.40, 1.79, 2.31 and 3.23, respectively, each for 15 days), which gradually induced SARA; those in recovery group were ad libitum fed green hay for 30 days after SARA. The three goats in control group were slaughtered on days 30, 60 (together with SARA goup) and 90 of feeding (together with recovery group). The results showed as follows: 1) plasma lipopolysaccharide (LPS) concentration was increased for a long time as a result of SARA challenge, and there was no significant difference between SARA group and recovery group (P>0.05). 2) Compared with control group, the contents of plasma tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 tended to be increased (P>0.05), and IL-2 content was significantly increased (P<0.05), but interferon-γ (IFN-γ) content was significantly decreased (P<0.05), and the contents of plasma IL-4, IL-8 and IL-10 tended to be decreased in SARA group (P>0.05); the contents of plasma growth hormone, insulin-like growth factor-Ⅰ and insulin were not changed (P>0.05), and the contents of prolactin and cortisol were significantly increased (P<0.05). 3) Compared with SARA group, the contents of plasma TNF-α, IL-1β, IL-2, IL-6 in recovery group were still persistently increased, in which IL-1β and IL-2 contents were significantly increased (P<0.05); plasma prolactin content stayed at the same level as SARA group (P>0.05), and plasma cortisol content was back to the same level as control group (P>0.05). These results indicate that SARA can increase plasma LPS concentration of dairy goats, which can lead to systemic immune and inflammatory responses, endocrine hormones alterations. These changes will result in stress of dairy goats for a long time.

Key words： SARA; dairy goat; cytokine; hormone

参考文献

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