中华绒螯蟹幼蟹对苏氨酸需求量的研究

王伟, 叶金云, 杨霞, 张易祥, 刘沛

doi:10.3969/j.issn.1006-267x.2015.02.018

动物营养学报 >

2015 , Vol. 27 >Issue 2: 476 - 484

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.02.018

水产营养 Aquaculture Nutrition

中华绒螯蟹幼蟹对苏氨酸需求量的研究

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1. 温州医科大学检验医学院、生命科学学院, 温州 325035;
2. 浙江省水生生物资源养护与开发技术研究重点实验室, 中国水产科学研究院水生动物繁育与营养重点实验室, 湖州师范学院, 湖州 313000;
3. 大连海洋大学, 大连 116023

王伟(1989-), 男, 安徽阜阳人, 硕士研究生, 从事水产动物营养与饲料研究.E-mail:tiexuedaqinww@126.com

收稿日期: 2014-08-08

网络出版日期: 2015-02-09

基金资助

公益性行业(农业)科研专项(201003020);浙江省淡水养殖重点科技创新团队项目(2010R50026-17)

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Threonine Requirement of Juvenile Chinese Mitten Crab (Eriocheir sinensis)

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1. School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China;
2. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding & Nutrition of Chinese Academy of Fishery Science, Huzhou University, Huzhou 313000, China;
3. Dalian Ocean University, Dalian 116023, China

Received date: 2014-08-08

Online published: 2015-02-09

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摘要

通过56 d的生长试验确定中华绒螯蟹(Eriocheir sinensis)幼蟹对苏氨酸的需求量.试验设计了6种以酪蛋白、明胶和晶体氨基酸为蛋白质源的等氮等能纯化饲料,通过添加晶体L-苏氨酸,使得饲料中苏氨酸含量分别为饲料干物质的0.86%(对照,无外源苏氨酸)、1.24%、1.61%、2.04%、2.42%、2.81%.选取初始体重为(0.36±0.00) g的中华绒螯蟹幼蟹360只,随机分为6组,每组3个重复,每个重复20只幼蟹.结果表明:随饲料中苏氨酸含量的升高,幼蟹的增重率、特定生长率以及消化酶(胃蛋白酶、胰蛋白酶、脂肪酶和淀粉酶)活力均先升高后降低,在苏氨酸含量为饲料干物质的1.61%时,各项指标均达到最大值,且此时饲料系数最低,蛋白质效率最高,且该组增重率、特定生长率显著高于0.86%、1.24%、2.42%和2.81%组(P<0.05),消化酶活力显著高于其他各组(P<0.05).饲料中苏氨酸含量对幼蟹的成活率及体成分无显著影响(P>0.05).幼蟹肝胰腺超氧化物歧化酶、碱性磷酸酶和酸性磷酸酶活力均随饲料中苏氨酸含量的升高呈先升高后降低趋势,并分别在苏氨酸含量为饲料干物质的1.61%、1.61%和2.42%时达到最大值.以增重率为评价指标进行折线回归模型分析,得到中华绒螯蟹幼蟹对饲料中苏氨酸的需求量为饲料干物质的1.59%(饲料蛋白质的3.98%).

关键词： 中华绒螯蟹; 苏氨酸; 需求量; 消化酶; 非特异性免疫指标

本文引用格式

王伟, 叶金云, 杨霞, 张易祥, 刘沛 . 中华绒螯蟹幼蟹对苏氨酸需求量的研究[J]. 动物营养学报, 2015 , 27(2) : 476 -484 . DOI: 10.3969/j.issn.1006-267x.2015.02.018

Abstract

A 56-day growth trial was conducted to determine the threonine requirement of juvenile Chinese mitten crab (Eriocheir sinensis). Six isonitrogenous and isoenergetic purified diets were formulated using casein, gelatin and crystalline amino acids as protein sources, and the threonine content in those diets was 0.86%(control, without extra threonine), 1.24%, 1.61%, 2.04%, 2.42% and 2.81% of dietary dry matter by adding crystalline L-threonine, respectively. A total of 360 juvenile Chinese mitten crab with the initial body weight of (0.36±0.00) g were chosen and randomly distributed into 6 groups with 3 replicates per group and 20 crabs per replicate. The results showed that with the increase of dietary threonine content, the weight gain rate (WGR), specific growth rate (SGR), and the activities of digestive enzymes (pepsin, trypsin, lipase and amylase) were firstly increased and then decreased, which reached their peaks in the 1.61% group, and the WGR and SGR in the 1.61% group were significantly higher than those in the 0.86%, 1.24%, 2.42% and 2.81% groups (P<0.05) and the activities of digestive enzymes in the 1.61% group were significantly higher than those in the other groups (P<0.05). Whereas, the feed conversion ratio (FCR) reached the lowest value in the 1.61% group, and the protein efficiency ratio (PER) reached the highest value in the same group. Dietary threonine content had no significant effects on survival rate (SR) and body composition (P>0.05). The activities of superoxide dismutase (SOD), alkaline phosphatase (AKP) and acid phosphatase (ACP) in hepatopancreas were firstly increased and then decreased, which reached their peaks in the 1.61%, 1.61% and 2.42% groups, respectively. Using WGR as the evaluation index, dietary threonine requirement of juvenile Chinese mitten crab is estimated to be 1.59% of dietary dry matter (3.98% of dietary protein) by broken-line regress model analysis.

Key words： juvenile Chinese mitten crab; threonine; requirement; digestive enzymes; non-specific immune indices

参考文献

[1] SMALL B C,SOARES J H,Jr.Quantitative dietary threonine requirement of juvenile striped bass Morone saxatilis[J].Journal of the World Aquaculture Society,1999,30(3):319-323.

[2] RAVI J,DEVARAJ K V.Quantitative essential amino acid requirements for growth of catla,Catla catla (Hamilton)[J].Aquaculture,1991,96(3/4):281-291.

[3] MILLAMENA O M,BAUTISTA M N,REYES O S,et al.Threonine requirement of juvenile marine shrimp Penaeus monodon[J].Aquaculture,1997,151(1/2/3/4):9-14.

[4] AHMED I,KHAN M A,JAFRI A K.Dietary threonine requirement of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)[J].Aquaculture Research,2004,35(2):162-170.

[5] WILSON R P,ALLEN O W,Jr,ROBINSON E H,et al.Tryptophan and threonine requirements of fingerling channel catfish[J].The Journal of Nutrition,1978,108(10):1595-1599.

[6] HELLAND S J,GRISDALE-HELLAND B.Dietary threonine requirement of Atlantic salmon smolts[J].Aquaculture,2011,321(3/4):230-236.

[7] THONG H T,LIEBERT F.Amino acid requirement of growing pigs depending on amino acid efficiency and level of protein deposition.2nd communication:threonine[J].Archives of Animal Nutrition,2004,58(2):157-168.

[8] YAMAMOTO T,UNUMA T,AKIYAMA T.The influence of dietary protein and fat levels on tissue free amino acid levels of fingerling rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2000,182(3/4):353-372.

[9] ZHOU Q C,WANG Y L,WANG H L,et al.Dietary threonine requirements of juvenile Pacific white shrimp,Litopenaeus vannamei[J].Aquaculture,2013,392-395:142-147.

[10] HUAI M Y,TIAN L X,LIU Y J,et al.Quantitative dietary threonine requirement of juvenile Pacific white shrimp,Litopenaeus vannamei (Boone) reared in low-salinity water[J].Aquaculture Research,2009,40(8):904-914.

[11] TESHIMA S,ALAM M S,KOSHIO S,et al.Assessment of requirement values for essential amino acids in the prawn,Marsupenaeus japonicus (Bate)[J].Aquaculture Research,2002,33(6):395-402.

[12] 林仕梅,罗莉,叶元土.中华绒螯蟹的营养需求[J].科学养鱼,2001(4):41-42.

[13] 叶金云,王友慧,郭建林,等.中华绒螯蟹对赖氨酸、蛋氨酸和精氨酸的需要量[J].水产学报,2010,34(10):1541-1548.

[14] 王树英,陈辉.中华绒螯蟹不同部位氨基酸的测定与分析[J].氨基酸和生物资源,1999,21(2):35-36.

[15] 张璐,陈立侨,洪美玲,等.中华绒螯蟹对11种饲料原料蛋白质和氨基酸的表观消化率[J].水产学报,2007,31(增刊):116-121.

[16] SANTIAGO C B,LOVELL R T.Amino acid requirements for growth of Nile tilapia[J].The Journal of Nutrition,1988,118(12):1540-1546.

[17] BORLONGAN I G.Arginine and threonine requirements of milkfish (Chanos chanos Forsskal) juveniles[J].Aquaculture,1991,93(4):313-322.

[18] BOREN R S,GATLIN D M.Dietary threonine requirement of juvenile red drum Sciaenops ocellatus[J].Journal of the World Aquaculture Society,1995,26(3):279-283.

[19] TIBALDI E,TULLI F.Dietary threonine requirement of juvenile european sea bass (Dicentrarchus labrax)[J].Aquaculture,1999,175(1/2):55-166.

[20] AHMED I,KHAN M A.Dietary lysine requirement of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)[J].Aquaculture,2004,235(1/2/3/4):499-511.

[21] ALAM M S,TESHIMA S,KOSHIO S,et al.Optimum dietary threonine level for juvenile Japanese flounder Paralichthys olivaceus[J].Asian Fisheries Science,2003,16(1/2):175-184.

[22] SHIVANANDA M H,VARGHESE T J.Quantitative dietary requirement of threonine for the growth of the Indian major carp,Labeo rohita (Hamilton)[J].Journal of Aquaculture in the Tropics,1996,11:1-7.

[23] 何志刚,艾庆辉,麦康森,等.鲈幼鱼对饲料中苏氨酸的需要量[J].水产学报,2012,36(1):124-131.

[24] 潘鲁青,刘泓宇,肖国强.甲壳动物幼体消化酶研究进展[J].中国水产科学,2006,13(3):492-501.

[25] 冯琳.苏氨酸对幼建鲤消化吸收能力和抗病力以及组织器官中蛋白质调控信号分子TOR表达的影响[D].博士学位论文.雅安:四川农业大学,2010:37-51.

[26] 胡晓霞.生长中期草鱼的苏氨酸需要量研究[D].硕士学位论文.雅安:四川农业大学,2012:21-49.

[27] 洪杨.苏氨酸对生长后期草鱼消化吸收功能和抗氧化能力影响的研究[D].硕士学位论文.雅安:四川农业大学,2012:11-41.

[28] 林林,丁美丽,孙舰军,等.有机污染提高对虾对病原菌易感性研究[J].海洋学报,1998,20(1):90-93.

[29] 丁美丽,林林,李光友,等.有机污染对中国对虾体内外环境影响的研究[J].海洋与湖沼,1997,28(1):7-12.

[30] 王雷,李光友,毛远兴,等.口服免疫型药物对养殖中国对虾病害防治作用的研究[J].海洋与湖沼,1994,25(5):486-492.

[31] 孙舰军,丁美丽.氨氮对中国对虾抗病力的影响[J].海洋与湖沼,1999,30(3):267-272.

[32] WELLEN K E,THOMPSON C B.Cellular metabolic stress:considering how cells respond to nutrient excess[J].Molecular Cell,2010,40(2):323-332.

[33] CHENG T C.The role of lysosomal hydrolases in molluscan cellular response to immunologic challenge[M]//BULLA L A,Jr,CHENG T C.Invertebrate models for biomedical research.New York:Springer,1978:59-71.

[34] 刘树青,江晓路,牟海津,等.免疫多糖对中国对虾血清溶菌酶、磷酸酶和过氧化物酶的作用[J].海洋与湖沼,1999,30(3):278-283.

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