反刍动物营养 Ruminant Nutrition

胎次和泌乳阶段对荷斯坦奶牛血液乳成分前体物及泌乳相关激素含量的影响

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  • 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
韩英东(1988-),男,山东菏泽人,硕士研究生,从事反刍动物营养研究。E-mail:hanyingdong2008@126.com

收稿日期: 2014-11-21

  网络出版日期: 2015-04-21

基金资助

973计划课题(2011CB100805);基本科研业务费(2013ywf-zd-3);863计划课题(2012AA101905)

Effects of Parity and Stage of Lactation on Milk Component Precursor and Lactation Related Hormone Contents in Blood of Holstein Dairy Cows

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  • State Key Laboratory of Animal Nutrition, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2014-11-21

  Online published: 2015-04-21

摘要

本文旨在研究胎次和泌乳阶段对荷斯坦奶牛血液乳成分前体物及泌乳相关激素含量的影响。选取不同泌乳阶段的1~4胎奶牛60头荷斯坦奶牛饲喂相同全混合日粮,每15 d采集血液。血液检测参数有葡萄糖(GLU)、甘油三酯(TG)、非酯化脂肪酸(NEFA)、β-羟丁酸(BHBA)、总氨基酸(T-AA)5种乳成分合成前体物及甲状腺素(T4)、生长激素(GH)、皮质醇(COR)、胰岛素(INS)、催乳素(PRL)、孕酮(PRG)6种激素的含量。利用SAS 9.2中Proc Univeriate程序进行正态分布检验,利用Proc Mixed线性模型评估胎次、泌乳阶段的固定效应。结果表明:1)胎次可显著影响泌乳奶牛血液INS及PRL含量(P<0.05),而对血液COR、GH、PRG、T4及5个乳成分前体物含量无显著影响(P>0.05)。2)随着泌乳时间延长,血液GLU含量逐渐递增,且泌乳早期(0~21 d)显著低于其他泌乳阶段(P<0.05),而泌乳早期血液BHBA和NEFA含量显著高于其他泌乳阶段(P<0.05),同时血液T-AA含量在泌乳中期(22~100 d)显著高于其他泌乳阶段(P<0.05);泌乳阶段对奶牛血液COR、GH、PRG、T4和INS含量均有显著的影响(P<0.05),但对血液PRL含量无显著影响(P>0.05),其中泌乳前期COR含量显著高于泌乳中期和后期(201~305 d)(P<0.05),而泌乳后期的GH、PRG和T4含量均较高。本方法能提高利用生化检测指标评估泌乳荷斯坦奶牛代谢状态的准确性,可以作为奶牛养殖者监测奶牛健康状态的工具。

本文引用格式

韩英东, 杨亮, 潘晓花, 辛海瑞, 熊本海 . 胎次和泌乳阶段对荷斯坦奶牛血液乳成分前体物及泌乳相关激素含量的影响[J]. 动物营养学报, 2015 , 27(4) : 1135 -1144 . DOI: 10.3969/j.issn.1006-267x.2015.04.017

Abstract

This experiment was conducted to study the effects of parity and stage of lactation on milk component precursor and lactation related hormone contents in blood of Holstein dairy cows. Sixty Holstein dairy cows from parity 1 to 4 with different stages of lactation were selected and fed the same total mixed ration. The blood samples were collected every 15 days and then were evaluated for the contents of glucose (GLU), triglyceride (TG), non-esterified fatty acid (NEFA), β-hydroxybutyric acid (BHBA), total amino acid (T-AA), thyroxine (T4), growth hormone (GH), cortisol (COR), insulin (INS), prolactin (PRL) and progesterone (PRG). Data was analyzed for their Gaussian distribution using Proc Univeriate, and was statistically processed by Proc Mixed using a linear model to assess the fixed effects of parity, stage of lactation in SAS 9.2. The results were showed as follows: 1) blood INS and PRL contents were significantly affected by parity (P<0.05), while blood COR, GH, PRG, T4 and five MCP contents were not significantly affected (P>0.05). 2) With the extending of lactation stage, blood GLU content was increased gradually, and early lactation (0 to 21 days) was significantly lower than the other lactation stages (P<0.05), while blood BHBA and NEFA contents of early lactation were significantly higher than those of the other lactation stages (P<0.05), meanwhile, blood T-AA content of mid-lactation (22 to 100 days) was significantly higher than that of the other lactation stages (P<0.05); blood COR, GH, PRG, T4 and INS contents were significantly influenced by stage of lactation (P<0.05), but no significant effect was found on blood PRL content (P>0.05), among those, blood COR content of early lactation was significantly higher than that of mid- and late lactation (201 to 305 days)(P<0.05), and blood GH, PRG and T4 contents were higher at late lactation. The outcomes of this work will improve the accuracy of the biochemical profile as a tool for dairy practitioners to assess the metabolic status of lactating Holstein dairy cows.

参考文献

[1] FLINT D J, GARDNER M.Evidence that growth hormone stimulates milk synthesis by direct action on the mammary gland and that prolactin exerts effects on milk secretion by maintenance of mammary deoxyribonucleic acid content and tight junction status[J].Endocrinology, 1994, 135(3):1119-1124.

[2] LACASSE P, LOLLIVIER V, DESSAUGE F, et al.New developments on the galactopoietic role of prolactin in dairy ruminants[J].Domestic Animal Endocrinology, 2012, 43(2) :154-160.  

[3] MENZIES K K, LEE H J, LEFÈVRE C, et al.Insulin, a key regulator of hormone responsive milk protein synthesis during lactogenesis in murine mammary explants[J].Functional & Integrative Genomics, 2010, 10(1):87-95.  

[4] CHILLIARD Y, DELAVAUD C, BONNET M.Leptin expression in ruminants:nutritional and physiological regulations in relation with energy metabolism[J].Domestic Animal Endocrinology, 2005, 29(1):3-22.  

[5] RUSSELL K E, ROUSSEL A J.Evaluation of the ruminant serum chemistry profile[J].Veterinary Clinics of North America:Food Animal Practice, 2007, 23(3):403-426.  

[6] KADZERE C T, MURPHY M R, SILANIKOVE N et al.Heat stress in lactating dairy cows:a review[J].Livestock Production Science, 2002, 77(1):59-91.  

[7] OSPINA P A, NYDAM D V, STOKOL T, et al.Associations of elevated nonesterified fatty acids and β-hydroxybutyrate concentrations with early lactation reproductive performance and milk production in transition dairy cattle in the northeastern United States[J].Journal of Dairy Science, 2010, 93(4):1596-1603.  

[8] QUIROZ-ROCHA G F, LEBLANC S J, DUFFIELD T F, et al.Reference limits for biochemical and hematological analytes of dairy cows one week before and one week after parturition[J].Canadian Veterinary Journal:La Revue Veterinaire Canadienne, 2009, 50(4):383-388.

[9] 中国奶业协会.NY/T 34—2004 奶牛饲养标准[S].北京:中国农业出版社, 2005.

[10] SOLBERG H E.Approved recommendation (1987) on the theory of reference values.Part 5.Statistical treatment of collected reference values.Determination of reference limits[J].Clinica Chimica Acta, 1987, 170(2/3):S13-S32.

[11] 熊本海, 易渺, 杨琴, 等.中国北方荷斯坦奶牛乳成分及相关指标的季节性与胎次变化规律研究[J].畜牧兽医学报, 2013, 44(1):31-37.

[12] COZZI G, RAVAROTTO L, GOTTARDO F, et al.Short communication:reference values for blood parameters in Holstein dairy cows:effects of parity, stage of lactation, and season of production[J].Journal of Dairy Science, 2010, 94(8):3895-3901.

[13] DOORNENBAL H, TONG A K W, MURRAY N L.Reference values of blood parameters in beef cattle of different ages and stages of lactation[J].Canadian Journal of Veterinary Research-Revue Canadienne de Recherche Vétérinaire, 1988, 52(1):99-105.  

[14] FREEMAN M E, KANYICSKA B, LERANT A, et al.Prolactin:structure, function, and regulation of secretion[J].Physiological Reviews, 2000, 80(4):1523-1631 .

[15] TUCKER H A.Hormones, mammary growth, and lactation:a 41-year perspective[J].Journal of Dairy Science, 2000, 83(4):874-884.  

[16] BARBER M C, TRAVERS M T, FINLEY E, et al.Growth-hormone-prolactin interactions in the regulation of mammary and adipose-tissue acetyl-CoA carboxylase activity and gene expression in lactating rats[J].Biochemical Journal, 1992, 285(Pt 2):469-475.

[17] BASSETT N S, CURRIE M J, KLEMPT M, et al.The effects of ovine placental lactogen and bovine growth hormone on hepatic and mammary gene expression in lactating sheep[J].Growth Hormone & IGF Research, 1998, 8(6):439-446.  

[18] SEVI A, TAIBI L, ALBENZIO H, et al.Effect of parity on milk yield, composition, somatic cell count, renneting parameters and bacteria counts of Comisana ewes[J].Small Ruminant Research, 2000, 37(1/2):99-107.

[19] 王小龙, 切·玛克鲁.对围产期乳牛某些血液生化成分变化的研究[J].中国畜牧兽医杂志, 1993(7):16-17.

[20] CANT J P, TROUT D R, QIAO F, et al.Milk synthetic response of the bovine mammary gland to an increase in the local concentration of arterial glucose[J].Journal of Dairy Science, 2002, 85(3):494-503.  

[21] ZHAO F Q, KEATINGA F.Expression and regulation of glucose transporters in the bovine mammary gland[J].Journal of Dairy Science, 2007, 90S:E76-E86.

[22] GRVNWALDT E G, GUEVARA J C, ESTÉVEZ J C, et al.Biochemical and haematological measurements in beef cattle in Mendoza plain rangelands (Argentina)[J].Tropical Animal Health and Production, 2005, 37(6):527-540.  

[23] BLUM J W, KUNZ P, LEUENBERGER H, et al.Thyroid hormones, blood plasma metabolites and haematological parameters in relationship to milk yield in dairy cows[J].Animal Science, 1983, 36(1):93-104.

[24] VAN KNEGSEL A T, VAN DEN BRAND H, GRAAT E A, et al.Dietary energy source in dairy cows in early lactation:metabolites and metabolic hormones[J].Journal of Dairy Science, 2007, 90(3):1477-1485.  

[25] GRUMMER R R.Nutritional and management strategies for the prevention of fatty liver in dairy cattle[J].The Veterinary Journal, 2008, 176(1):10-20.  

[26] WALTERS A H, PRYOR A W, BAILEY T Let al.Milk yield, energy balance, hormone, follicular and oocyte measures in early and mid-lactation Holstein cows[J].Theriogenology, 2002, 57(2):949-961.  

[27] PURDIEN G, TROUT D R, POPPI D P, et al.Milk synthetic response of the bovine mammary gland to an increase in the local concentration of amino acids and acetate[J].Journal of Dairy Science, 2008, 91(1):218-228.  

[28] 孙满吉.阴外动脉灌注乙酸盐, 氨基酸和葡萄糖对奶山羊乳腺营养物质和乳成分影响的研究[R].呼和浩特:内蒙古农业大学, 2008.

[29] WINTERMANTEL T M, BOCK D, FLEIG V, et al.The epithelial glucocorticoid receptor is required for the normal timing of cell proliferation during mammary lobuloalveolar development but is dispensable for milk production[J].Molecular Endocrinology, 2005, 19(2):340-349.  

[30] DAVIS S R, GLUCKMAN P D, HART I C, et al.Effects of injecting growth hormone or thyroxine on milk production and blood plasma concentrations of insulin-like growth factors Ⅰ andⅡ in dairy cows[J].Journal of Endocrinology, 114(1):17-24.

[31] KAHL S, CAPUCO A V, BITMA N J.Serum concentrations of thyroid hormones and extrathyroidal thyroxine-5'-monodeiodinase activity during lactation in the rat[J].Experimental Biology and Medicine, 1987, 184(2):144-150.  

[34] BAUMAN D E.Bovine somatotropin:review of an emerging animal technology[J].Journal of Dairy Science, 1991, 75(12):3432-3451.
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