动物营养学报 >

2015 , Vol. 27 >Issue 4: 1278 - 1292

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.04.035

研究简报 Short Communications

花鲈和西伯利亚鲟生长激素/类胰岛素样生长因子-Ⅰ轴相关基因全长cDNA的克隆和序列分析

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  • 1. 中国农业科学院饲料研究所, 国家水产饲料安全评价基地, 北京 100081;
    2. 中粮营养健康研究院有限公司, 动物营养与饲料中心, 北京 102209;
    3. 农业部饲料生物技术重点开放实验室, 北京 100081
张志勇(1988-),男,湖北武穴人,硕士研究生,从事水产动物营养与饲料研究。E-mail:985461147@qq.com

收稿日期: 2014-10-29

  网络出版日期: 2015-04-21

基金资助

国家自然科学基金(31101907,31372539);公益性行业(农业)专项经费项目(201203015);北京市现代农业产业技术体系(SCGWZJ 20151103-1)

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Cloning and Sequence Analysis of Full Length cDNAs of Growth Hormone/Insulin-Like Growth Factor-Ⅰ Axis Relative Genes of Japanese Seabass (Lateolabrax japonicus) and Siberian Sturgeon (Acipenser baerii Brandt)

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  • 1. The National Aquatic Feed Safety Assessment Center, Feed Institute Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Animal Nutrition and Feed Center, Food Nutrition and Health Research Institute Co., Ltd., Beijing 102209, China;
    3. Key Laboratory of Feed Biotechnology, The Ministry of Agriculture, Beijing 100081, China

Received date: 2014-10-29

  Online published: 2015-04-21

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摘要

本研究采用反转录聚合酶链式反应(RT-PCR)和cDNA末端快速扩增(RACE)技术克隆花鲈(Lateolabrax japonicus)和西伯利亚鲟(Acipenser baerii Brandt)生长激素(GH)和生长激素受体(GHR)cDNA全长序列。结果显示:花鲈GH(GenBank登录号JQ995145)cDNA全长949 bp,开放阅读框615 bp,编码204个氨基酸,预测分子质量为23.06 ku,与其他物种的相似性为36.0%~90.2%;花鲈GHRⅠ(GenBank登录号JX402001)cDNA全长3 070 bp,开放阅读框1 911 bp,编码637个氨基酸,预测分子质量为70.79 ku,与其他物种的相似性为32.2%~86.2%;花鲈GHRⅡ(GenBank登录号JQ995146)cDNA全长2 926 bp,开放阅读框1 749 bp,编码582个氨基酸,预测分子质量为64.42 ku,与其他物种的相似性为30.8%~81.4%。西伯利亚鲟GH(GenBank登录号JX003684)cDNA全长999 bp,开放阅读框645 bp,编码214个氨基酸,预测分子质量为24.14 ku,与其他物种的相似性为38.3%~70.1%;西伯利亚鲟GHR(GenBank登录号JX003685)cDNA全长2 283 bp,开放阅读框1 716 bp,编码571个氨基酸,预测分子质量为63.41 ku,与其他物种的相似性为39.5%~49.2%。花鲈和西伯利亚鲟GH和GHR cDNA全长序列的获得为进一步研究鱼类GH/类胰岛素样生长因子-Ⅰ(IGF-Ⅰ)轴对生长发育的调控机制奠定了科学基础。

关键词： 花鲈; 西伯利亚鲟; 基因克隆; GH/IGF-Ⅰ轴; 生长激素; 生长激素受体

本文引用格式

张志勇, 薛敏, 王嘉, 吴秀峰, 郑银桦, 韩芳 . 花鲈和西伯利亚鲟生长激素/类胰岛素样生长因子-Ⅰ轴相关基因全长cDNA的克隆和序列分析[J]. 动物营养学报, 2015 , 27(4) : 1278 -1292 . DOI: 10.3969/j.issn.1006-267x.2015.04.035

Abstract

The reverse transcription-phosphatase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) methods were used in this experiment to clone the full-length sequences of cDNAs for growth hormone (GH) and GH receptor (GHR) of Japanese seabass (Lateolabrax japonicus) and Siberian sturgeon (Acipenser baerii Brandt). The results showed as follows: the full-length GH cDNA of Japanese seabass (GenBank accession No. JQ995145) was 949 bp, consisting of an open reading frame (ORF) of 615 bp encoding a protein of 204 amino acids with a theoretical molecular weight of 23.06 ku, and shared 36.0% to 90.2% similarity with other species. The full-length GHRⅠ cDNA of Japanese seabass (GenBank accession No. JX402001) was 3 070 bp, consisting of an ORF of 1 911 bp encoding a protein of 637 amino acids with a theoretical molecular weight of 70.79 ku, and shared 32.2% to 86.2% similarity with other species. The full-length GHRⅡ cDNA of Japanese seabass (GenBank accession No. JQ995146) was 2 926 bp, consisting of an ORF of 1 749 bp encoding a protein of 582 amino acids with a theoretical molecular weight of 64.42 ku, and shared 30.8% to 81.4% similarity with other species. The full-length GH cDNA of Siberian sturgeon (GenBank accession No. JX003684) was 999 bp, consisting of an ORF of 645 bp encoding a protein of 214 amino acids with a theoretical molecular weight of 24.14 ku, and shared 38.3% to 70.1% similarity with other species. The full-length GHR cDNA of Siberian sturgeon (GenBank accession No. JX003685) was 2 238 bp, consisting of an ORF of 1 716 bp encoding a protein of 571 amino acids with a theoretical molecular weight of 63.41 ku, and shared 39.5% to 49.2% with other species. The full-length sequences of cDNAs for GH and GHR of Japanese seabass and Siberian sturgeon will provide a scientific basis for further study on the regulatory mechanism of GH/IGF-Ⅰ axis on growth and development of fish.

Key words： Japanese seabass (Lateolabrax japonicus); Siberian sturgeon (Acipenser baerii Brandt); gene cloning; GH/IGF-Ⅰ axis; growth hormone; growth hormone receptor

参考文献

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