研究简报 Short Communications

猪源肠道嗜酸乳杆菌的分离、鉴定与驯化

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  • 江西农业大学生物科学与工程学院, 南昌市发酵应用技术重点实验室, 南昌 330045
胡国才(1986-),男,江西乐平人,硕士,从事微生物资源利用研究。E-mail:450201089@qq.com

收稿日期: 2014-11-30

  网络出版日期: 2015-04-21

基金资助

江西省农业开发项目

Isolation, Identification and Acclimation of Lactobacillus acidophilus from Intestinal Tracts of Piglets

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  • College of Bioscience and Bioengineering of Jiangxi Agricultural University, Nanchang Key Laboratory of Fermentation Application Technology, Nanchang 330045, China

Received date: 2014-11-30

  Online published: 2015-04-21

摘要

本文旨在分离筛选适于在猪肠道生长的嗜酸乳杆菌(Lactobacillus acidophilus)菌株。选用仔猪新鲜粪便,利用MRS培养基,以平板划线法分离纯化菌株;采用形态、生理生化和16S rRNA基因序列分析对分离菌株进行鉴定;通过逐步提高培养基酸度、胆盐浓度和培养温度,对分离菌株进行驯化。结果表明:1)经分离纯化获得的菌株La-5,被鉴定为嗜酸乳杆菌。2)驯化后的菌株La-5c在pH=1.5~3时存活率比原始菌株La-5高出36%~44%,极低pH(1.5)条件下仍有37%的存活率。3)在0.03%~0.3%胆盐中的存活率为85%~90%,而原始菌株La-5为28%~53%。4)在50 ℃时的存活率为66.1%,而原始菌株La-5 < 0.01%。由此可见,与仔猪粪便分离纯化的嗜酸乳杆菌原始菌株La-5比较,驯化获得的菌株La-5c具有耐低pH、高浓度胆盐及高温的特性。

本文引用格式

胡国才, 黄静, 吴晓玉 . 猪源肠道嗜酸乳杆菌的分离、鉴定与驯化[J]. 动物营养学报, 2015 , 27(4) : 1316 -1325 . DOI: 10.3969/j.issn.1006-267x.2015.04.039

Abstract

The paper aimed at isolation and screening of a strain, Lactobacillus acidophilus, which can be localized well in intestinal tracts of pigs. Fresh feces of piglets were selected for bacteria source, MRS medium was used, and streak plate techniques were applied for isolation and purification. Bacteria were identified through morphological observation, biochemical and physiological tests, as well as 16S rRNA gene sequence analyis. Bacteria strains were acclimated in gradually elevated acidity and concentration of bile salt in medium and culture temperatures. The results showed as follows: 1) a strain named La-5 was isolated and purified, which was identified as Lactobacillus acidophilus. 2) Strain La-5c screened from original strain La-5 by adapting had a 36% to 44% increment of survival rate compared with the original strain La-5 under the range of pH 1.5 to 3. The strain La-5 had a survival rate of 37% at pH=1.5. 3) In the range of 0.03% to 0.3% of bile salt concentration, strain La-5c showed a survival rate of 85% to 90%, while strain La-5 only had 28% to 53%. 4) At the culture temperature of 50 ℃, survival rate of strain La-5c was 66.1%, but it is <0.01% for the original strain La-5. It is concluded that the acclimated strain La-5c had elevated tolerances to low pH, high concentration of bile salt and high culture temperature compared with the original Lactobacillus acidophilus La-5 from piglet feces.

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