饲料中硒含量对方格星虫稚虫生长、体成分、组织硒含量及相关酶活性的影响

许明珠, 张琴, 童潼, 董兰芳, 杨家林, 蒋艳, 黄国强

doi:10.3969/j.issn.1006-267x.2015.06.009

动物营养学报 >

2015 , Vol. 27 >Issue 6: 1733 - 1739

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.009

水产营养 Aquaculture Nutrition

饲料中硒含量对方格星虫稚虫生长、体成分、组织硒含量及相关酶活性的影响

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广西壮族自治区海洋研究所, 广西海洋生物技术重点实验室, 北海 536000

许明珠(1988—),女,湖南湘潭人,硕士研究生,从事水产动物营养与饲料的研究。E-mail: xumingzhulian@163.com

收稿日期: 2014-12-23

网络出版日期: 2015-06-17

基金资助

国家自然科学基金项目(31402304);广西科学研究与技术开发计划(桂科转13129024,桂科合1346011-12,桂科合14125008-2-20);广西自然科学基金项目(2011GXNSFB018057)

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Effects of Dietary Selenium Content on Growth, Body Composition, Tissue Selenium Content and Related Enzyme Activities of Juvenile Peanut Worm, Sipunculus nudus Linnaeus

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Guangxi Institute of Oceanology, Key Laboratory of Marine Biotechnology of Guangxi, Beihai 536000, China

Received date: 2014-12-23

Online published: 2015-06-17

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摘要

本试验旨在研究饲料中硒含量对方格星虫(Sipunculus nudus Linnaeus)稚虫生长、体成分、组织硒含量及相关酶活性的影响。选取平均体重为(16.12±0.26) mg的方格星虫稚虫苗种7 200条,随机分为6组,每组3个重复,每个重复400条。此6组方格星虫稚虫分别饲喂饲料中硒含量为0.19、0.37、0.63、0.82、1.06和1.29 mg/kg的饲料8周。结果表明:饲料中硒含量对方格星虫稚虫增重率、特定生长率和成活率都有显著的影响(P<0.05)。增重率和特定生长率随着饲料中硒含量的增加而上升,在1.29 mg/kg组达到最大,但成活率在此组则最低。饲料中硒含量对虫体的粗脂肪含量有显著影响(P<0.05),虫体粗脂肪含量随着饲料中硒含量的增加而增加。虫体的水分、粗灰分和粗蛋白质含量各组间差异不显著(P>0.05)。饲料中硒含量对体壁和虫体硒含量都有显著影响(P<0.05),两者都随着饲料中硒含量的增加而增加。此外,饲料中硒含量的变化还对虫体谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性有显著影响(P<0.05),其中谷胱甘肽过氧化物酶的活性随着饲料中硒含量的增加而提高,而谷胱甘肽还原酶的活性随着饲料中硒含量的增加而降低。由此得出,饲料中适宜含量的硒可以促进方格星虫稚虫的生长,从而增加方格星虫的养殖产量。

关键词： 方格星虫稚虫; 硒; 生长; 体成分; 谷胱甘肽过氧化物酶; 谷胱甘肽还原酶; 组织硒含量

本文引用格式

许明珠, 张琴, 童潼, 董兰芳, 杨家林, 蒋艳, 黄国强 . 饲料中硒含量对方格星虫稚虫生长、体成分、组织硒含量及相关酶活性的影响[J]. 动物营养学报, 2015 , 27(6) : 1733 -1739 . DOI: 10.3969/j.issn.1006-267x.2015.06.009

Abstract

This trial was conducted to investigate the effects of dietary selenium content on growth, body composition, tissue selenium content and related enzyme activities of juvenile peanut worm, Sipunculus nudus Linnaeus. A total of 7 200 juvenile peanut worm with the average body weight of (16.12±0.26) mg were randomly divided into 6 groups with 3 replicates per group and 400 juveniles per replicate. The juveniles in those 6 groups were fed diets with six selenium contents (0.19, 0.37, 0.63, 0.82, 1.06 and 1.29 mg/kg, respectively) for 8 weeks. The results showed that the dietary selenium content had significant effects on weight gain rate (WGR), specific growth rate (SGR) and survival ratio (SR) of juvenile peanut worm (P<0.05). With the increase of dietary selenium content, the WGR and SGR were increased, and the highest values were observed in 1.29 mg/kg group, but the lowest value of SR was also found in this group. The dietary selenium content had a significant effect on body lipid content (P<0.05), the body lipid content showed an increasing trend as the dietary selenium content increasing. No significant differences were observed in body moisture, crude protein and ash contents among all groups (P>0.05). And dietary selenium content could significantly affected the contents of selenium in body wall and body (P<0.05), and those were increased with the dietary selenium content increasing. Moreover, dietary selenium content could significantly affected body glutathione peroxidas (GSH-Px) and glutathione reductase (GR) activities of juvenile peanut worm (P<0.05). With the of dietary selenium content increasing, the GSH-Px activity was increased and GR activity was declined. In conclusion, the suitable dietary selenium content can improve the growth of juvenile peanut worm, thus increase the cultured production of peanut worm.

Key words： juvenile peanut worm; selenium; growth; body composition; GSH-Px; GR; tissue selenium content

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