分子营养 Molecular Nutrition

饲粮纤维水平对高黎贡山仔猪与杜洛克仔猪生产性能、小肠形态学、小肠黏膜金属硫蛋白1及组织急性期蛋白基因表达影响的比较研究

展开
  • 1. 云南农业大学动物科学技术学院, 云南省动物营养与饲料重点实验室, 昆明 650201;
    2. 云南省畜牧兽医科学院, 昆明 650224
李美荃(1986—),女,黑龙江哈尔滨人,博士研究生,从事猪的营养与免疫基础研究。E-mail: limeiquan2010@163.com

收稿日期: 2014-12-30

  网络出版日期: 2015-06-17

基金资助

云南省重大科技专项基金(2012ZA018);云南现代农业生猪产业技术体系2013[云财教]160号;云南省科技创新人才计划项目(2011HB035)

Comparison Study of Effects of Dietary Fiber Level on Performance, Intestinal Morphology, mRNA Expression of Metallothioneins 1 in Intestinal Mucosa and Acute Phase Proteins in Tissues of Gaoligongshan and Duroc Piglets

Expand
  • 1. Yunnan Animal Nutrition and Feed Laboratory, College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    2. Yunnan Animal Science and Veterinary Institute, Kunming 650224, China

Received date: 2014-12-30

  Online published: 2015-06-17

摘要

本试验旨在比较饲粮纤维水平对杜洛克仔猪、高黎贡山仔猪生产性能、小肠形态学、小肠黏膜金属硫蛋白1(MT1)和组织急性期蛋白(APPs)基因表达的影响。选用35日龄高黎贡山断奶仔猪和杜洛克断奶仔猪各24头(公母各占1/2)为研究对象,试验设对照组和试验组(每组12个重复,每个重复1头猪),分别饲喂纤维水平为3.53%和7.04%饲粮,饲养至60日龄结束。考察仔猪生产性能,观察小肠形态学变化,检测十二指肠、空肠、回肠黏膜MT1 mRNA相对表达量以及肝脏、胰脏、脾脏、肾脏、十二指肠、空肠、回肠7种组织中4种APPs[C-反应蛋白(CRP)、触珠蛋白(HP)、α1-酸性糖蛋白(AGP)和血清淀粉样蛋白A(SAA)]mRNA相对表达量。结果表明:饲粮纤维水平为7.04%时,高黎贡山仔猪日增重、日采食量显著高于杜洛克仔猪(P<0.05),料重比显著低于杜洛克仔猪(P<0.05);高黎贡山仔猪十二指肠、空肠、回肠的绒毛长度极显著大于杜洛克仔猪(P<0.01);十二指肠、空肠隐窝深度极显著低于杜洛克仔猪(P<0.01);十二指肠、空肠、回肠绒毛长度/隐窝深度极显著大于杜洛克仔猪(P<0.01)。饲喂纤维水平为7.04%饲粮的高黎贡山仔猪十二指肠黏膜中MT1 mRNA相对表达量极显著高于饲喂纤维水平为3.53%饲粮的高黎贡山仔猪及饲喂纤维水平为7.04%或3.53%饲粮的杜洛克仔猪(P<0.01)。被测组织中均能检测出CRPHPAGPSAA mRNA的表达。饲粮纤维水平为7.04%时,绝大部分组织的APPs mRNA相对表达量均为高黎贡山仔猪显著高于杜洛克仔猪(P<0.05)。上述结果表明,高黎贡山仔猪相对杜洛克仔猪更加适应高纤维水平饲粮;高黎贡山仔猪通过上调MT1及APPs mRNA的表达,增加仔猪的抗病能力,从而改善仔猪的生产性能。

本文引用格式

李美荃, 信爱国, 张春勇, 安清聪, 王荣蛟, 潘洪彬, 陈克嶙, 郭荣富 . 饲粮纤维水平对高黎贡山仔猪与杜洛克仔猪生产性能、小肠形态学、小肠黏膜金属硫蛋白1及组织急性期蛋白基因表达影响的比较研究[J]. 动物营养学报, 2015 , 27(6) : 1759 -1768 . DOI: 10.3969/j.issn.1006-267x.2015.06.012

Abstract

This study was conducted to compare the effects of dietary fiber level on performance, intestinal morphology, mRNA expression of metallothioneins 1 (MT1) in intestinal mucosa and acute phase proteins (APPs) in tissues of Gaoligongshan and Duroc piglets. Each twenty four (half male and half female) Duroc and Gaoligongshan weaner piglets of 35 days old were selected. They divided into control group and experimental group with 12 replicates per group and 1 piglet per replicate. Piglets in the control group and the experimental group were fed the diets with the fiber level was 3.53% and 7.04%, respectively. The experiment finished at 60 days old. The performance, intestinal morphological change, mRNA relative expression of MT1 in duodenum, jejunum, ileum mucosa and four kinds of APPs [C-reactive protein (CRP), haptoglobin (HP), α1-acid glycoprotein (AGP) and serum amyloid A (SAA)] in liver, pancreas, spleen, kidney, duodenum, jejunum and ileum were detected. The results showed as follows: when dietary fiber level was 7.04%, daily gain and daily feed intake of Gaoligongshan piglets was significantly higher than those of Duroc piglets (P<0.05), while feed conversion ratio was significantly lower than that of Duroc piglets (P<0.05); villus length of duodenum, jejunum and ileum of Gaoligongshan piglets was significantly higher than that of Duroc piglets (P<0.01); crypt depth of duodenum and jejunum was significantly lower than that of Duroc piglets (P<0.01); villus length/crypt depth of duodenum, jejunum and ileum was significantly higher than that of Duroc piglets (P<0.01). When dietary fiber level was 7.04%, MT1 mRNA relative expression in duodenum mucosa of Gaoligongshan piglets fed the diet with 7.04% fiber level was significantly higher than that of Gaoligongshan piglets fed the diet with 3.53% fiber level and Duroc piglets fed the diets with 7.04% or 3.53% fiber level (P<0.01). CRP, HP, AGP and SAA mRNA expression could be detected in the measured tissues. When dietary fiber level was 7.04%, APPs mRNA relative expression in most tissues of Gaoligongshan piglets was significantly higher than that of Duroc piglets. These results suggest that Gaoligongshan piglets is better adapted to high fiber level diets than Duroc piglets; disease resistance is improved of Gaoligongshan piglets by increasing MT1 and APPs mRNA expression, thereby improving the performance of piglets.

参考文献

[1] 安清聪,张春勇,李美荃,等.谷氧还蛋白1和硫氧还蛋白1基因在高黎贡山猪不同组织中表达规律及维生素E对其在氧化应激细胞中表达的影响[J].动物营养学报,2013,25(8):1825-1835.

[2] VAREL V H,YEN J T.Microbial perspective on fiber utilization by swine[J].Journal of Animal Science,1997,75(10):2715-2722.

[3] URRIOLA P E,STEIN H H.Comparative digestibility of energy and nutrients in fibrous feed ingredients fed to Meishan and Yorkshire pigs[J].Journal of Animal Science,2012,90(3):802-812.  

[4] HEDEMANN M S,ESKILDSEN M,LAERKE H N,et al.Intestinal morphology and enzymatic activity in newly weaned pigs fed contrasting fiber concentrations and fiber properties[J].Journal of Animal Science,2006,84(6):1375-1386.

[5] HERMES R G,MOLIST F,YWAZAKI M,et al.Effect of dietary level of protein and fiber on the productive performance and health status of piglets[J].Journal of Animal Science,2009,87(11):3569-3577.  

[6] 陈恒灿,马黎,陈克嶙,等.丙氨酰谷氨酰胺二肽对早期断奶仔猪抗氧化能力及肠道保护的研究[J].畜牧兽医学报,2011,42(2):251-259.

[7] PFAFFL M W.A new mathematical model for relative quantification in real-time RT-PCR[J].Nucleic Acids Research,2001,29(9):e45

[8] CASTILLO M,MARTÍN-ORÙE S M,TAYLOR-PICKARD J A,et al.Use of mannan-oligosaccharides and zinc chelate as growth promoters and diarrhea preventative in weaning pigs:Effects on microbiota and gut function[J].Journal of Animal Science,2008,86(1):94-101.

[9] URRIOLA P E,STEIN H H.Effects of distillers dried grains with solubles on amino acid,energy,and fiber digestibility and on hindgut fermentation of dietary fiber in a corn-soybean meal diet fed to growing pigs[J].Journal of Animal Science,2010,88(4):1454-1462.  

[10] JAYASURYA A,BAY B H,YAP W M,et al.Proliferative potential in nasopharyngeal carcinoma:correlations with metallothionein expression and tissue zinc levels[J].Carcinogenesis,2000,21(10):1809-1812.  

[11] 李振华,田子强,丛斌,等.食管癌组织中MT-1、-2与-3的表达差异及临床意义[J].中国肿瘤临床,2012,39(15):1091-1095.

[12] 林林.MTBag-1在胃癌中的表达[D].硕士学位论文.石家庄:河北医科大学,2003.

[13] 谢忆山,王蕊,陶卫平.金属硫蛋白在大肠癌及正常黏膜组织中阳性表达的临床意义[J].肿瘤防治研究,2009,36(10):886-891.

[14] PIEIRO C,PIEIRO M,MORALES J,et al.Pig-MAP and haptoglobin concentration reference values in swine from commercial farms[J].The Veterinary Journal,2009,179(1):78-84.  

[15] 许美芳,李永明,齐珂珂,等.3种评价猪群健康的急性期蛋白相关性的研究[J].中国畜牧杂志,2013,49(1):57-60.

[16] SASAKI K,FUJITA I,HAMASAKI Y,et al.Differentiating between bacterial and viral infection by measuring both C-reactive protein and 2'-5'-oligoadenylate synthetase as inflammatory markers[J].Journal of Infection and Chemotherapy,2002,8(1):76-80.  

[17] MONTAGNE L, LE FLOC'H N,ARTURO-SCHAAN M,et al.Comparative effects of level of dietary fiber and sanitary conditions on the growth and health of weanling pigs[J].Journal of Animal Science,2012,90(8):2556-2569.  

[18] 鲁建云,吴志强,谭丽娜,等.结合珠蛋白mRNA及其蛋白在尖锐湿疣皮损中的表达[J].中南大学学报:医学版,2007,32(6):1020-1025.

[19] LANGLOIS M R,DELANGHE J R.Biological and clinical significance of haptoglobin polymorphism in humans[J].Clinical Chemistry,1996,42(10):1589-1600.

[20] QUAYE I K.Haptoglobin,inflammation and disease[J].Transactions of the Royal Society of Tropical Medicine and Hygiene,2008,102(8):735-742.  

[21] 许美芳,李永明,徐子伟.急性期蛋白及其用于动物健康状态的评价[J].家畜生态学报,2010,31(3):76-80.

[22] GUNNARSSON P,LEVANDER L,PHLSSON P,et al.The acute-phase protein α1-acid glycoprotein (AGP) induces rises in cytosolic Ca2+ in neutrophil granulocytes via sialic acid binding immunoglobulin-like lectins (Siglecs)[J].The FASEB Journal,2007,21(14):4059-4069.  

[23] 张建江,易著文,罗雪梅,等.血清C-反应蛋白、α1-酸性糖蛋白和前白蛋白的检测在儿童感染性疾病中的应用[J].中国综合临床,2006,22(5):470-472.

[24] URIELI-SHOVAL S,FINCI-YEHESKEL Z,DISHON S,et al.Expression of serum amyloid a in human ovarian epithelial tumors:implication for a role in ovarian tumorigenesis[J].Journal of Histochemistry and Cytochemistry,2010,58(11):1015-1023.  

[25] MCDONALD T L,LARSON M A,MACK D R,et al.Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum[J].Veterinary Immunology and Immunopathology,2002,83(3/4):203-211.
文章导航

/