饲粮中添加鼠李糖乳酸杆菌GG缓解轮状病毒感染诱导仔猪氧化应激的研究

汤俊, 王爱娜, 陈代文, 毛湘冰, 陈浩, 刘明辉, 余冰, 何军, 郑萍, 虞洁, 王曲圆

doi:10.3969/j.issn.1006-267x.2015.06.015

动物营养学报 >

2015 , Vol. 27 >Issue 6: 1787 - 1793

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.015

饲料营养 Feed Science and Technology

饲粮中添加鼠李糖乳酸杆菌GG缓解轮状病毒感染诱导仔猪氧化应激的研究

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1. 四川农业大学动物营养研究所, 动物抗病营养教育部重点实验室, 雅安 625014;
2. 潍坊工商职业学院, 诸城 262234

汤俊(1990—),女,重庆铜梁人,硕士研究生,研究方向为猪的营养。E-mail: 965092703@qq.com

收稿日期: 2015-01-21

网络出版日期: 2015-06-17

基金资助

国家自然科学基金青年科学基金项目(31201812);四川省科技支撑项目(2013NZ0056);十二五国家科技支撑计划(2011BAD26B02)

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Dietary Lactobacillus rhamnosus GG Supplementation Alleviates the Oxidant Stress Induced by Rotavirus in Weaned Piglets

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1. Institute of Animal Nutrition, Sichuan Agricultural University, Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Ya'an 625014, China;
2. Weifang Business Vocational College, Zhucheng 262234, China

Received date: 2015-01-21

Online published: 2015-06-17

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摘要

本研究旨在探讨鼠李糖乳酸杆菌GG(LGG)对轮状病毒(RV)感染诱导的断奶仔猪氧化应激的缓解作用。试验选取24头平均体重为(6.69±0.32) kg健康的21日龄断奶的"杜×长×大"去势公猪,采用2×2双因子试验设计,饲粮中添加0和10⁹ CFU/kg LGG;仔猪接受RV感染(灌服RV)或不感染(灌服无菌培养液)处理,RV感染于试验第15天早上进行。试验期为19 d。结果表明,RV感染显著提高了仔猪的腹泻指数和料重比(F/G)(P<0.05),显著提高了十二指肠、空肠、回肠和血清中丙二醛(MDA)水平(P<0.05),显著降低了空肠中总超氧化物歧化酶(T-SOD)及十二指肠、空肠、回肠和血清中谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05),显著降低了空肠和血清中总抗氧化能力(T-AOC)(P<0.05)。添加LGG显著影响了仔猪腹泻指数、F/G、空肠中MDA水平和T-SOD活性,及血清中GSH-Px活性(P<0.05),显著影响了空肠和血清中T-AOC(P<0.05);并可显著缓解RV感染诱导引起的仔猪腹泻率提高和空肠、血清T-AOC的下降(P<0.05)。综上所述,饲粮LGG的添加可以降低仔猪的料重比,并可缓解RV感染诱导的断奶仔猪腹泻,这可能源于LGG提高了仔猪机体(尤其是肠道)抗氧化能力,维持机体正常氧化还原平衡,继而增强仔猪的抗病力。

关键词： 鼠李糖乳酸杆菌; 断奶仔猪; 轮状病毒; 生产性能; 抗氧化能力

本文引用格式

汤俊, 王爱娜, 陈代文, 毛湘冰, 陈浩, 刘明辉, 余冰, 何军, 郑萍, 虞洁, 王曲圆 . 饲粮中添加鼠李糖乳酸杆菌GG缓解轮状病毒感染诱导仔猪氧化应激的研究[J]. 动物营养学报, 2015 , 27(6) : 1787 -1793 . DOI: 10.3969/j.issn.1006-267x.2015.06.015

Abstract

This experiment was aimed at investigate the effects of dietary Lactobacillus rhamnosus GG (LGG) supplementation on the oxidant stress induced by rotavirus (RV) challenge in weaned piglets. Twenty-four healthy crossbred (Duroc×Landrace×Yorkshire) castration piglets with body weight of (6.69±0.32) kg weaned at age of 21 days were used in the experiment. A 2×2 factorial design was used. Dietary supplementing LGG levels were 0 and 10⁹ CFU/kg, respectively. The piglets were orally infused with RV or not (sterilized MEM medium) on the morning of day 15. The experiment lasted for 19 days. The results showed that rotavirus challenge significantly enhanced diarrhea index, feed to gain ratio (F/G) and the malondialdehyde (MDA) level of the duodenum, jejunum, ileum and serum in the piglets (P<0.05), significantly inhibited the total superoxide dismutase (T-SOD) activity of the jejunum and the glutathione peroxidase (GSH-Px) activity of the duodenum, jejunum, ileum and serum in the piglets (P<0.05), and significantly reduced the total antioxidant capacity (T-AOC) of the jejunum and serum in the piglets (P<0.05). Dietary LGG supplementation significantly affected diarrhea index, F/G, the MDA level and T-SOD activity of the jejunum, the GSH-Px activity of serum and the T-AOC of jejunum and serum in the piglets (P<0.05). In addition, dietary LGG supplementation might, in a certain extent, significantly alleviate the effects of RV challenge on the diarrhea and antioxidant capacity of the piglets. These results suggest that dietary LGG supplementation reduces the diarrhea induced by rotavirus infection, and improves the F/G of the piglets, which can be due that dietary LGG supplementation may increase the antioxidant capacity of the body (intestine especially), inhibit the production of lipid peroxides and free radicals, and maintain the normal antioxidant system.

Key words： Lactobacillus rhamnosus GG; weaned piglets; rotavirus; performance; antioxidant capacity

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