动物营养学报 >

2015 , Vol. 27 >Issue 6: 1794 - 1803

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.016

饲料营养 Feed Science and Technology

果胶酶和纤维素酶对五龙鹅养分表观利用率、消化酶活性及排泄物指标的影响

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  • 1. 青岛农业大学动物科技学院, 青岛 266109;
    2. 青岛农业大学优质水禽研究所, 青岛 266109
殷太岳(1991—),男,山东青岛人,硕士研究生,从事单胃动物营养与饲料科学研究。E-mail: 296719683@qq.com

收稿日期: 2015-01-05

  网络出版日期: 2015-06-17

基金资助

国家水禽技术产业体系(CARS-43-11);青岛市科技计划项目(11-2-3-23-nsh)

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Effects of Pectinase and Cellulase on Nutrient Apparent Utilization, Digestive Enzyme Activities and Excretion Indexes of Wulong Geese

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  • 1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China;
    2. Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2015-01-05

  Online published: 2015-06-17

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摘要

本试验旨在研究高纤维饲粮中添加果胶酶和纤维素酶对鹅养分表观利用率、消化酶活性及排泄物指标的影响。选用1日龄五龙鹅216只,随机分为6组,每组3个重复,每个重复12只鹅。Ⅰ组饲喂高纤维基础饲粮,Ⅱ、Ⅲ、Ⅳ组分别在Ⅰ组饲粮基础上添加5 000、10 000、15 000 U/kg的果胶酶,Ⅴ组添加5 000 U/kg果胶酶和40 000 U/kg纤维素酶,Ⅵ组添加10 000 U/kg果胶酶和40 000 U/kg纤维素酶。试验期112 d。结果表明:1)Ⅴ、Ⅵ组干物质、粗蛋白质、粗纤维、酸性洗涤纤维、中性洗涤纤维和能量的表观利用率显著高于Ⅰ组(P<0.05),钙表观利用率极显著高于Ⅰ组(P<0.01);Ⅲ、Ⅳ组钙表观利用率显著高于Ⅰ组(P<0.05)。2)4周龄时,Ⅴ、Ⅵ组胰腺淀粉酶活性、十二指肠淀粉酶和脂肪酶活性显著高于Ⅰ组(P<0.05);16周龄时,Ⅴ、Ⅵ组胰腺淀粉酶和脂肪酶活性、十二指肠胰蛋白酶和脂肪酶活性显著高于Ⅰ组(P<0.05),Ⅴ、Ⅵ组十二指肠淀粉酶活性极显著高于Ⅰ组(P<0.01)。3)16周龄时,Ⅴ、Ⅵ组盲肠乳酸杆菌和双歧杆菌的数量显著高于Ⅰ组(P<0.05),产气荚膜梭菌的数量显著低于Ⅰ组(P<0.05)。4)各组排泄物中大肠杆菌数量及总氮、总磷含量无显著差异(P>0.05)。由此可见,饲粮添加果胶酶和纤维素酶可提高五龙鹅养分表观利用率和肠道酶活性,对排泄物指标无影响。

关键词： 果胶酶; 纤维素酶; 表观利用率; 肠道酶活性; 大肠杆菌

本文引用格式

殷太岳, 毛倩倩, 王宝维, 葛文华, 张名爱, 李文立 . 果胶酶和纤维素酶对五龙鹅养分表观利用率、消化酶活性及排泄物指标的影响[J]. 动物营养学报, 2015 , 27(6) : 1794 -1803 . DOI: 10.3969/j.issn.1006-267x.2015.06.016

Abstract

This experiment was conducted to study the effects of high fiber diet supplemented with pectinase and cellulase on nutrient apparent utilization, digestive enzyme activies and excretion indexes of Wulong geese. A total of 216 one-day-old Wulong geese were randomly divided into 6 groups with 3 replicates per group and 12 geese in each replicate. Geese in the group Ⅰ were fed the high fiber basal diet, and the others were fed the basal diet supplemented with 5 000 U/kg pectinase (group Ⅱ), 10 000 U/kg pectinase (group Ⅲ), 15 000 U/kg pectinase (group Ⅳ), 5 000 U/kg pectinase and 40 000 U/kg cellulose (group Ⅴ), 10 000 U/kg pectinase and 40 000 U/kg cellulose (group Ⅵ), respectively. The trial lasted for 112 days. The results showed as follows: 1) the apparent utilization of dry matter, crude protein, crude fiber, acid detergent fiber, neutral detergent fiber and energy of groups Ⅴ and Ⅵ was significantly higher than that of group Ⅰ (P<0.05), the calcium apparent utilization was significantly higher than that of group Ⅰ (P<0.01); the calcium apparent utilization of groups Ⅲ and Ⅳ was significantly higher than that of group Ⅰ (P<0.05). 2) At 4 weeks of age, the activity of amylase in pancreatic, amylase and lipase in duodenal of groups Ⅴ and Ⅵ was significantly higher than that of group Ⅰ (P<0.05); at 16 weeks of age, the activity of amylase and lipase in pancreatic, trypsin and lipase in duodenal of groups Ⅴ and Ⅵ was significantly higher than that of group Ⅰ (P<0.05), the activity of amylase in duodenal of groups Ⅴ and Ⅵ was significantly higher than that of group Ⅰ (P<0.01). 3) At 16 weeks of age, the number of Lactobacilli and Bifidobacteria in cecal of groups Ⅴ and Ⅵ was significantly higher than that of group Ⅰ (P<0.05), the number of Clostridium perfringens was significantly lower than that of group Ⅰ (P<0.05). 4)There were no significant effects on the number of Escherichia coli and the contents of total nitrogen and total phosphorus in fecal of Wulong geese (P>0.05). In conclusion, dietary supplemented with pectinase and cellulase can improve the nutrient apparent utilization and intestinal enzymes activity of Wulong geese,but has no effect on excretion indexes.

Key words： pectinase; cellulase; nutrient apparent utilization; intestinal enzymes activity; Escherichia coli

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