研究简报 Short Communications

植物乳杆菌GF103对生长猪生长性能、营养物质消化率及粪便微生物数量的影响

展开
  • 1. 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081;
    2. 北京大兴区动物卫生监督管理局, 北京 102600
张乃锋(1974—),男,山东章丘人,副研究员,博士,研究方向为动物营养与饲料科学。E-mail: zhangnaifeng@caas.cn

收稿日期: 2015-01-05

  网络出版日期: 2015-06-17

基金资助

现代农业产业技术体系北京市创新团队建设资金(GWZJ-2009-08);农业部引进国际先进农业科学技术计划(948计划)项目(2011-G7-5);国家留学基金(201403250010)

Effects of Lactobacillus plantarum GF103 on the Growth Performance, Nutrient Digestibility and Fecal Microbiota Numbers in Growing Pigs

Expand
  • 1. Feed Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, China;
    2. Bureau of Animal Health Supervision of Daxing District, Beijing 102600, China

Received date: 2015-01-05

  Online published: 2015-06-17

摘要

本试验旨在研究植物乳杆菌(Lactobacillus plantarum)GF103对生长猪生长性能、营养物质消化率及粪便微生物数量的影响。利用随机区组试验设计方法,将平均体重(26.36±1.93) kg的300头生长猪分为5个组,每组3个重复,每重复20头,重复内生长猪日龄一致、公母各占1/2。对照(CON)组饲喂基础饲粮,抗生素(PC)组在基础饲粮中添加杆菌肽锌40 mg/kg,植物乳杆菌1~3(T1、T2和T3)组分别在基础饲粮中添加1×109、5×109、1×1010 CFU/kg的植物乳杆菌GF103。试验预试期7 d,正试期30 d。结果表明:1)T1组生长猪平均日增重和平均日采食量均显著高于其他各组(P <0.05),料重比则显著低于其他各组(P <0.05)。T2和T3组生长猪的平均日增重、平均日采食量和料重比与PC和CON组相比差异均不显著(P > 0.05)。各组间生长猪腹泻率无显著差异(P>0.05)。2)T1、T3及CON组的总能、干物质、有机物质、粗蛋白质、粗脂肪消化率显著高于PC和T2组(P<0.05),各组之间的中性洗涤纤维和磷的消化率无显著差异(P>0.05)。3﹚T1、T2和T3组生长猪粪便中乳酸菌数量显著高于CON和PC组(P<0.05),并且粪便乳酸菌数量随着植物乳杆菌GF103添加剂量的增加而增加;各组生长猪粪便大肠杆菌数量无显著差异(P>0.05);T1组生长猪粪便中乳酸菌/大肠杆菌与T3组无显著差异(P>0.05),显著高于其他各组(P<0.05)。结果提示,在生长猪饲粮中添加1×109 CFU/kg植物乳杆菌GF103,能够改善生长猪肠道菌群环境,提高生长猪生长速度和饲料利用效率。

本文引用格式

张乃锋, 王杰, 崔凯, 沈陈, 祁敏丽, 柴建民, 单瑛琦, 刁其玉 . 植物乳杆菌GF103对生长猪生长性能、营养物质消化率及粪便微生物数量的影响[J]. 动物营养学报, 2015 , 27(6) : 1853 -1860 . DOI: 10.3969/j.issn.1006-267x.2015.06.022

Abstract

The purpose of this present study was to evaluate the effects of dietary Lactobacillus plantarum GF103 on the growth performance, nutrient digestibility and fecal microbiota numbers in growing pigs. A total of 300 growing pigs, whose average body weight was (26.36±1.93) kg, were allotted to 1 of 5 groups. The diets of these groups were basal control diet (CON group), positive control diet containing 40 mg/kg zinc bacitracin (PC group), and experimental diets containing 1×109, 5×109 or 1×1010 CFU/kg Lactobacillus plantarum GF103, respectively (T1, T2 and T3 groups). The pre-test period lasted for 7 days, and the trial period lasted for 30 days. The results showed as follows: 1) the average daily gain (ADG) and average daily feed intake (ADFI) of T1 group were significantly greater, whereas the ratio of feed to gain (F/G) of T1 group was significantly lower than those of other groups (P<0.05). The ADG, ADFI and F/G of T2 and T3 groups had no significant differences compared with CON or PC groups (P > 0.05). The rate of diarrhea had no significant difference between all groups (P > 0.05). 2) The digestibility of total energy (TE), dry matter (DM), organic matter (OM), crude protein (CP) and ether extract (EE) of T1, T3 and CON groups were significantly greater than those of PC and T2 groups (P<0.05). The digestibility of neutral detergent fiber (NDF) and phosphorus (P) among groups had no significant differences (P > 0.05). 3) The number of fecal Lactobacilli of T1, T2 and T3 groups was significantly greater than that of CON and PC groups (P<0.05), and it was increased with the increase of dietary content of Lactobacillus plantarum GF103. There was no difference of E. coli among all groups (P > 0.05). However, the ratio of Lactobacilli to E. coli of T1 group had no significant difference with T3 group, and it was greater than that of other groups (P<0.05). In conclusion, Lactobacillus plantarum GF103, at a dietary concentration of 1×109 CFU/kg, can improve the environment of intestinal microbiota, and may serve as a potential alternative to antibiotic growth promoters to improve the growth performance in growing pigs.

参考文献

[1] 索成.植物乳杆菌ZJ316对断奶仔猪的益生作用[D].硕士学位论文.杭州:浙江工商大学,2012.

[2] LEE J S,AWJI E G,LEE S J,et al.Effect of Lactobacillus plantarum CJLP243 on the growth performance and cytokine response of weaning pigs challenged with enterotoxigenic Escherichia coli[J].Journal of Animal Science,2012,90(11):3709-3717.  

[3] LÄHTEINEN T,LINDHOLM A,RINTTILÄ T,et al.Effect of Lactobacillus brevis ATCC 8287 as a feeding supplement on the performance and immune function of piglets[J].Veterinary Immunology and Immunopathology,2014,158(1/2):14-25.

[4] SUDA Y,VILLENA J,TAKAHASHI Y,et al.Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs[J].BMC Immunology,2014,15(1):24-41.  

[5] HUANG C H,QIAO S Y,LI D F,et al.Effects of lactobacilli on the performance,diarrhea incidence,VFA concentration and gastrointestinal microbial flora of weaning pigs[J].Asian-Australasian Journal of Animal Sciences,2004,17(3):401-409.  

[6] CHEN Y J,MIN B J,CHO J H,et al.Effects of dietary Bacillus-based probiotic on growth performance,nutrients digestibility,blood characteristics and fecal noxious gas content in finishing pigs[J].Asian-Australasian Journal of Animal Sciences,2006,19(4):587-592.  

[7] CHEN Y J,SON K S,MIN B J,et al.Effects of dietary probiotic on growth performance,nutrients digestibility,blood characteristics and fecal noxious gas content in growing pigs[J].Asian-Australasian Journal of Animal Sciences,2005,18(10):1464-1468.  

[8] 张董燕,季海峰,王四新,等.猪源唾液乳杆菌对生长猪生长性能、粪中微生物数量及血清指标的影响[J].动物营养学报,2014,26(3):725-731.

[9] CHESSON A.Probiotics and other intestinal mediators[M]//COLE D,WISIMAN J,VARLEY M Y.Pinciples of Pig Science.Loughborough:Nottingham University Press,1994:197-214.

[10] 周盟,张乃锋,董晓丽,等.益生菌对断奶仔猪生长及消化性能的影响[J].饲料工业,2013,34(2):18-21.

[11] 董晓丽,张乃锋,周盟,等.复合菌制剂对断奶仔猪生长性能、粪便微生物和血清指标的影响[J].动物营养学报,2013,25(6):1285-1292.

[12] TAKAHASHI S,EGAWA Y,SIMOJO N,et al.Oral administration of Lactobacillus plantarum strain Lq80 to weaning piglets stimulates the growth of indigenous lactobacilli to modify the lactobacillal population[J].The Journal of General and Applied Microbiology,2007,53(6):325-332.  

[13] GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science,2010,129(1/2/3):95-103.

[14] MATSUKI T,WATANABE K,FUJIMOTO J,et al.Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces[J].Applied and Environmental Microbiology,2002,68(11):5445-5451.  

[15] NOUSIAINEN J,JAVANAINEN P,SETÄLÄ J,et al.Lactic acid bacteria microbiological and functional aspects[M]//SALMINEN S,VON WRIGHT A,OUWEHAND A.Lactic acid bacteria as animal probiotics.New York:Marcel Dekker Inc.,2004:547-580.

[16] YU H F,WANG A N,LI X J,et al.Effect of viable Lactobacillus fermentum on the growth performance,nutrient digestibility and immunity of weaned pigs[J].Journal of Animal and Feed Sciences,2008,17(1):61-69.

[17] MODESTO M,D'AIMMO M R,STEFANINI I,et al.A novel strategy to select Bifidobacterium strains and prebiotics as natural growth promoters in newly weaned pigs[J].Livestock Science,2009,122(2/3):248-258.

[18] MIKKELSEN L L,NAUGHTON P J,HEDEMANN M S,et al.Effects of physical properties of feed on microbial ecology and survival of Salmonella enterica serovar Typhimurium in the pig gastrointestinal tract[J].Applied and Environmental Microbiology,2004,70(6):3485-3492.  

[19] MIKKELSEN L L,JAKOBSEN M,JENSEN B B.Effects of dietary oligosaccharides on microbial diversity and fructo-oligosaccharide degrading bacteria in faeces of piglets post-weaning[J].Animal Feed Science and Technology,2003,109(1/2/3/4):133-150.

[20] DE CHAMPS C,MARONCLE N,BALESTRINO D,et al.Persistence of colonization of intestinal mucosa by a probiotic strain,Lactobacillus casei subsp.rhamnosus Lcr35,after oral consumption[J].Journal of Clinical Microbiology,2003,41(3):1270-1273.  

[21] GARDINER G E,CASEY P G,CASEY G,et al.Relative ability of orally administered Lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract[J].Applied and Environmental Microbiology,2004,70(4):1895-1906.  

[22] WANG Y,CHO J H,CHEN Y J,et al.The effect of probiotic BioPlus 2B® on growth performance,dry matter and nitrogen digestibility and slurry noxious gas emission in growing pigs[J].Livestock Science,2009,120(1/2):35-42.

[23] DAVIS M E,BROWN D C,BAKER A,et al.Effect of direct-fed microbial and antibiotic supplementation on gastrointestinal microflora,mucin histochemical characterization,and immune populations of weanling pigs[J].Livestock Science,2007,108(1/2/3):249-253.

[24] LIM H S,KIM B H,PAIK I K.Effects of natufermen® supplementation to the diet on the perfermance of weanling pigs[J].Journal of Animal Science and Technology,2004,46(6):981-988.  

[25] 张董燕,季海峰,刘辉,等.猪源唾液乳杆菌对生长猪氮表观消化率及血清指标的影响[J].中国畜牧杂志,2013,49(7):60-62.

[26] 张常明,李路胜,王修启,等.乳酸菌对断奶仔猪生产性能及免疫力的影响[J].华南农业大学学报,2006,27(3):81-84.

[27] 王志祥,乔家运,王自恒,等.乳酸杆菌对断奶仔猪生长性能、养分表观消化率和消化酶活性的影响[J].西北农林科技大学学报:自然科学版,2006,34(4):23-27.

[28] KORNEGAY E T,RISLEY C R.Nutrient digestibilities of a corn-soybean meal diet as influenced by Bacillus products fed to finishing swine[J].Journal of Animal Science,1996,74(4):799-805.

[29] SHON K S,HONG J W,KWON O S,et al.Effects of Lactobacillus reuteri-based direct-fed microbial supplementation for growing-finishing pigs[J].Asian Australasian Journal of Animal Sciences,2005,18(3):370-374.  

[30] XUAN Z N,KIM J D,HEO K N,et al.Study on the development of a probiotics complex for weaned pigs[J].Asian-Australasian Journal of Animal Sciences,2001,14(10):1425-1428.  

[31] STAVRIC S,KORNEGAY E T.Microbial probiotics for pigs and poultry[M]//WALLACE R,CHESSON A.Biotechnology in Animal Feeds and Animal Feeding.Weinheim:Wiley-VCH Verlag GmbH,1995:205-231.

[32] CASTILLO M,MARTÍN-ORÙE S M,MANZANILLA E G,et al.Quantification of total bacteria,enterobacteria and lactobacilli populations in pig digesta by real-time PCR[J].Veterinary Microbiology,2006,114(1/2):165-170.

[33] CASTILLO M,MARTÍN-ORÙE S M,ANGUITA M,et al.Adaptation of gut microbiota to corn physical structure and different types of dietary fibre[J].Livestock Science,2007,109(1):149-152.
文章导航

/