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递增注射脂多糖诱导慢性免疫应激对仔猪生长性能的影响

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  • 1. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
    2. 安徽农业大学动物科技学院, 合肥 230036;
    3. 安徽农业大学生命科学学院, 合肥 230036
贾安峰(1989—),男,安徽六安人,硕士研究生,从事环境与营养研究。E-mail: 714725789@qq.com

收稿日期: 2015-01-23

  网络出版日期: 2015-06-17

基金资助

国家"973"重点基础研究发展计划(2012CB124700);中国农业科学院创新工程(ASTIP-IAS07)资助

Effects of Chronic Immunological Stress Induced by Increasing Administration of Lipopolysaccharide on Growth Performance of Piglets

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  • 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
    3. College of Life Science, Anhui Agricultural University, Hefei 230036, China

Received date: 2015-01-23

  Online published: 2015-06-17

摘要

本试验旨在研究递增30%注射脂多糖(LPS)诱导仔猪慢性免疫应激的效果及对仔猪生长性能、营养物质表观消化率的影响。选取16头断奶仔猪,随机分为2组(对照组、LPS组),单笼饲养。LPS组隔天静脉注射LPS,初始注射剂量80 μg/kg BW,随后逐次递增30%,对照组注射相应剂量的生理盐水,试验期15 d。研究发现:仔猪注射LPS第1、5天血浆白细胞介素-1β和皮质醇含量极显著高于对照组(P<0.01),第9天血浆皮质醇有高于对照组的趋势(P=0.10),整个试验期内血浆结合珠蛋白含量显著高于对照组(P<0.05)。与对照组相比,注射LPS仔猪在试验的3个阶段(1~4 d、5~8 d和9~15 d)平均日增重分别降低了40.00%(P<0.01)、29.52%(P<0.05)和19.30%(P<0.05),平均日采食量分别降低了25.09%(P=0.01)、23.15%(P<0.05)和19.47%(P<0.05),增重耗料比与对照组无显著差异(P>0.05)。与对照组相比,注射LPS仔猪饲粮干物质表观消化率下降了5.43%(P<0.05),能量表观消化率有降低的趋势(P=0.06),粗脂肪和粗蛋白质表观消化率略有降低(P>0.10)。试验结果表明,递增30%注射LPS成功诱导仔猪慢性免疫应激,降低了仔猪平均日采食量和营养物质表观消化率,抑制了仔猪生长。

本文引用格式

贾安峰, 冯京海, 张敏红, 李泽阳, 常玉, 徐胜胜, 何川 . 递增注射脂多糖诱导慢性免疫应激对仔猪生长性能的影响[J]. 动物营养学报, 2015 , 27(6) : 1868 -1874 . DOI: 10.3969/j.issn.1006-267x.2015.06.024

Abstract

This experiment was conducted to study the effects of gradually increasing 30% of lipopolysaccharide (LPS) by injecting on the chronic immunological stress and on growth performance and nutrient apparent digestibility of piglets. Sixteen weaned piglets were randomly allotted into 2 groups (control and LPS groups), and were individually housed in metabolic cages. Saline (control) or LPS were injected at 48 h intervals with an initial dosage of 80 μg/kg BW, increased by 30% at each following injections. The experiment lasted for 15 d. The results showed that the contents of interleukin-1β and cortisol in plasma of the LPS group were significantly higher than those of control group on days 1 and 5 (P<0.01), and there was a trend that the content of cortisol in plasma of LPS group was higher than that of control group (P=0.10). Moreover, the content of haptoglobin in plasma was significantly higher than that of control group during the whole experimental period (P<0.05). Compared with the control group, during three phases of this experiment (from 1 to 4 days, 5 to 8 days and 9 to 15 days), the average daily gain of LPS group was decreased by 40.00% (P<0.01), 29.52% (P<0.05) and 19.30% (P<0.05), respectively. For the average daily feed intake was decreased by 25.09% (P=0.01), 23.15% (P<0.05) and 19.47% (P<0.05), respectively. At last, no significant difference was found in gain to feed ratio (P>0.05). Compared with control group, the apparent digestibility of dry matter in LPS group was decreased by 5.43% (P<0.05), and gross energy apparent digestibility had a decreasing trend (P=0.06), apparent digestibility of crude protein and ether extract were decreased slightly (P>0.10). In conclusion, increasing of 30% injection of LPS successfully induces the chronic immunological stress, and reduces feed intake and nutrient apparent digestibility of piglet, as well as inhibites the growth of piglets.

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