动物营养学报 >

2015 , Vol. 27 >Issue 6: 1883 - 1890

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.026

研究简报 Short Communications

戊酸雌二醇对雌性巴马香猪脂质代谢的影响及其作用机制

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  • 1. 中国科学院大学亚热带农业生态研究所, 动物生态营养与健康养殖联合实验室, 长沙 410125;
    2. 湖南农业大学动物科学技术学院, 长沙 410128
蒋国礼(1988—),男,湖南衡阳人,硕士研究生,从事畜禽营养生理研究。E-mail: 850021872@qq.com

收稿日期: 2015-01-22

  网络出版日期: 2015-06-17

基金资助

国家自然科学基金资助项目(31272462,30972118,31301985)

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Effect of Estradiol Valerate on Lipid Metabolism of Female Bama Xiang-Pigs and Its Mechanism

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  • 1. Laboratory of Animal Nutrition and Human Health, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2015-01-22

  Online published: 2015-06-17

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摘要

本试验旨在研究戊酸雌二醇对雌性巴马香猪脂质代谢的影响及其作用机制。采用单因子试验设计,选取20头健康的35日龄雌性巴马香猪随机分为2组,即对照组与试验组,每组10个重复,每个重复1头猪。对照组饲喂基础饲粮,试验组饲喂在基础饲粮中添加0.66 mg/kg戊酸雌二醇(相当于0.5 mg/kg雌二醇)的试验饲粮,试验周期为120 d。结果表明:1)饲粮添加戊酸雌二醇显著降低了雌性巴马香猪末体重、体增重及体脂肪率(P<0.05)。2)饲粮添加戊酸雌二醇显著降低了雌性巴马香猪血液葡萄糖、甘油三酯及低密度脂蛋白含量(P<0.05)。3)与对照组相比,试验组雌性香猪背部皮下脂肪中脂肪酸合成酶(FAS)、乙酰辅酶-A羧化酶(ACC)及脂蛋白脂肪酶(LPL)的mRNA相对表达水平显著降低(P<0.05),激素敏感脂肪酶(HSL)、脂肪甘油三酯脂酶(ATGL)及雌激素受体-β(ER-β)的mRNA相对表达水平显著升高(P<0.05),过氧化物酶体增殖因子受体-γ(PPAR-γ)、过氧化物酶体增殖因子受体-α(PPAR-α)及雌激素受体-α(ER-α)的mRNA相对表达水平无显著差异(P>0.05);腹部皮下脂肪中的FAS、ACC及LPL的mRNA相对表达水平显著降低(P<0.05),PPAR-α、HSL、ATGL、ER-α及ER-β的mRNA相对表达水平显著升高(P<0.05),PPAR-γ的mRNA相对表达水平无显著差异(P>0.05);肾周脂肪中的PPAR-γ、FAS、ER-α及ER-β的mRNA相对表达水平显著降低(P<0.05),HSL、ATGL及LPL的mRNA相对表达水平显著升高(P<0.05),ACC及PPAR-α的mRNA相对表达水平无显著差异(P>0.05)。由此可见,戊酸雌二醇可以有效地控制雌性巴马香猪的体增重及脂肪沉积,并且能有效地改善血糖及血脂水平。戊酸雌二醇可能通过2个途径来调节雌性香猪白色脂肪组织中的脂质代谢:其一,通过对脂肪合成及脂肪分解相关基因表达的调节来影响脂质代谢;其二,通过对雌激素受体基因表达的调节来调控LPL的表达,进而影响脂肪的合成代谢。

关键词： 雌性巴马香猪; 戊酸雌二醇; 体脂肪率; 甘油三酯; 脂质代谢; 雌激素受体

本文引用格式

蒋国礼, 范觉鑫, 张彬, 李丽立, 印遇龙 . 戊酸雌二醇对雌性巴马香猪脂质代谢的影响及其作用机制[J]. 动物营养学报, 2015 , 27(6) : 1883 -1890 . DOI: 10.3969/j.issn.1006-267x.2015.06.026

Abstract

To investigate the effect of estradiol valerate on lipid metabolism of female Bama Xiang-pigs and its mechanism, a total of 20 healthy female Bama Xiang-pigs of 35 days old were selected and randomly divided into two groups (control group and experimental group) with each group had 10 replicates and each replicate had 1 pig. The pigs in the control group were fed a basal diet, while the pigs in the experimental group were fed the basal diet supplemented with 0.66 mg/kg estradiol valerate (equivalent to 0.5 mg/kg estradiol) for 120 d. The results showed as follows: 1) diets supplemented with estradiol valerate significantly decreased the final body weight, body weight gain and body fat percentage of female Bama Xiang-pigs (P<0.05). 2) Diets supplemented with estradiol valerate significantly reduced the contents of glucose (GLU), triglyceride (TG) and low density lipoprotein (LDL) in blood of female Bama Xiang-pigs (P<0.05). 3) Compared with control group, the mRNA relative expression levels of fatty acid synthetase (FAS), acetyl CoA carboxylase (ACC) and lipoprteinlipase (LPL) in dorsal subcutaneous adipose of female Bama Xiang-pigs in experimental group were significantly decreased (P<0.05), and the mRNA relative expression levels of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL) and estrogen receptor-β (ER-β) were significantly increased (P<0.05), while the mRNA relative expression levels of peroxisome proliferators-activated receptor-γ (PPAR-γ), peroxisome proliferators-activated receptor-α (PPAR-α) and estrogen receptor-α (ER-α) were not significantly different (P>0.05); the mRNA relative expression levels of FAS, ACC and LPL in abdominal subcutaneous adipose were significantly decreased (P<0.05), and the mRNA relative expression levels of PPAR-α, HSL, ATGL, ER-α and ER-β were significantly increased (P<0.05), while the mRNA relative expression levels of PPAR-γ was not significantly different (P>0.05); the mRNA relative expression levels of PPAR-γ, FAS, ER-α and ER-β in perirenal adipose were significantly decreased (P<0.05), and the mRNA relative expression levels of HSL, ATGL and LPL were significantly increased (P<0.05), while the mRNA relative expression levels of ACC and PPAR-α were not significantly different (P>0.05). Those results indicate that estradiol valerate can control the body weight gain and fat deposition and improve the levels of blood GLU and TG of female Bama Xiang-pigs effectively. In addition, there may be exist two ways for estradiol valerate to regulate the lipid metabolism, one is that influencing the lipid metabolism in different white adipose tissues by regulating the expression of genes related to lipogenic and lipolytic metabolism, the other one is that modulating the expression of LPL by regulating the expression of estrogen receptors, then to affect the lipogenic metabolism.

Key words： female Bama Xiang-pig; estradiol valerate; body fat percentage; triglyceride; lipid metabolism; estrogen receptor

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