动物营养学报 >

2015 , Vol. 27 >Issue 6: 1976 - 1982

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.06.038

研究简报 Short Communications

竹鼠肠道中厌氧纤维素降解菌的分离与鉴定

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  • 1. 广西大学动物科学技术学院, 南宁 530005;
    2. 南宁学院, 南宁 530200
曹晓燕(1985—),女,山东临沂人,硕士研究生,从事生物技术在饲料中应用研究。E-mail: helpwater@163.com

收稿日期: 2015-01-05

  网络出版日期: 2015-06-17

基金资助

中柬越英国际合作项目(DelPHE ROUND 5,DelPHE project 852)

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Isolation and Identification of Anaerobic Cellulolytic Bacteria in Intestine of Rhizomyidae

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  • 1. College of Animal Science and Technology of Guangxi University, Nanning 530005, China;
    2. Nanning University, Nanning 530200, China

Received date: 2015-01-05

  Online published: 2015-06-17

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摘要

本试验旨在从竹鼠肠道中分离厌氧纤维素降解菌并鉴定。取中华竹鼠盲肠内容物稀释,采用含2种不同纤维素[微晶纤维素(MCC)和羧甲基纤维素钠(CMC-Na)]的培养基在厌氧条件下对纤维素降解菌进行初筛,后又经以纤维二糖为唯一碳源的培养基的多次复筛,选出的菌株进行经形态学和分子生物学的鉴定。结果表明:经过初筛和复筛,筛选出了2株厌氧纤维素降解菌。初筛培养基采用MCC和CMC-Na最终筛选出的2株纤维素降解菌的纤维素酶活力分别为6.599和5.268 U/mL。菌株经PCR鉴定、测序,用所得序列构建系统发育树,得出这2株菌与酪酸梭状芽孢杆菌(Clostridium butyricum)具有很高的同源性,达99%,二者均属于梭菌属(Clostridium)。本试验从竹鼠盲肠中成功筛选出2株纤维素降解菌,经鉴定确定属于梭菌属。

关键词： 竹鼠; 盲肠; 厌氧; 纤维素降解菌; 16S rDNA

本文引用格式

曹晓燕, 汪珊如, 徐雯, 徐高跷, 张胜男, 王士长, 梁世忠 . 竹鼠肠道中厌氧纤维素降解菌的分离与鉴定[J]. 动物营养学报, 2015 , 27(6) : 1976 -1982 . DOI: 10.3969/j.issn.1006-267x.2015.06.038

Abstract

In order to isolate and identify anaerobic cellulolytic bacteria in intestine of rhizomyidae, cecum content of Rnizomyssinehsis was collected and diluted. Culture mediums with different kinds of cellulose [microcrystalline cellulose (MCC) and sodium carboxymethyl cellulose (CMC-Na), respectively] were used for preliminary screening of cellulolytic bacteria under anaerobic condition, and then multiple screening using culture medium with cellobiose as the only carbon source was carried out. The screened strains were identified by the methods of morphology and molecular biology. The results showed as follows: after preliminary screening and screening, two strains of anaerobic cellulolytic bacteria were isolated. After preliminary screening on culture medium with MCC and CMC-Na, the cellulolytic enzyme activity of final screened strains was 6.599 and 5.268 U/mL, respectively. After PCR identification and sequencing, the sequence was used to construct phylogenetic tree, and high homology was found between the two strains and Clostridium butyricum, which reached 99%. The two strains belonged to Clostridium. In conclusion, two strains of anaerobic cellulolytic bacteria are successfully screened from cecum in of rhizomyidae, and are belonged to Clostridium.

Key words： rhizomyidae; cecum; anaerobic; cellulolytic bacteria; 16S rDNA

参考文献

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