猪营养 Swine Nutrition

生长猪空肠液中主要消化酶活性与饲粮养分消化率的相关性研究

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  • 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193
王钰明(1991—),男,陕西咸阳人,硕士研究生,从事饲料营养价值的评定。E-mail:wudixiaoming@163.com

收稿日期: 2015-04-14

  网络出版日期: 2015-10-15

基金资助

国家自然科学基金(31172215);中国农业科学院科技创新工程(ASTIP-IAS07);科技部创新方法工作专项(2009IM033100)

Correlation Study between Digestive Enzyme Activity of Jejunal Fluid and Nutrient Digestibility of Diets in Growing Pigs

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  • State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese academy of Agriculture Science, Beijing 100193, China

Received date: 2015-04-14

  Online published: 2015-10-15

摘要

本试验旨在探讨猪空肠消化液中主要消化酶的活性与饲粮养分消化率间的相关性,为饲粮养分消化影响因素的研究提供参考。试验分为2组,分别饲喂玉米-豆粕型饲粮和玉米-玉米干酒糟及其可溶物(DDGS)-米糠粕型饲粮,每组设16个重复。测定饲粮和粪样的总能和干物质、粗蛋白质含量及空肠液中主要消化酶的活性,然后对每种饲粮的养分消化率与猪空肠液中主要消化酶的活性进行简单相关分析和典型相关分析。结果表明:1)2种饲粮下,生长猪个体间4种主要消化酶活性的变异系数均在20%以上,而饲粮干物质、粗蛋白质和总能消化率的变异系数均在1.5%以内;2)猪空肠液中淀粉酶、胰蛋白酶和糜蛋白酶的活性与饲粮养分消化率间无显著相关性(|r|≤0.51;P>0.05),而脂肪酶活性与玉米-豆粕型饲粮养分消化率呈显著正相关(r≥0.56;P<0.05),与玉米-玉米DDGS-米糠粕型饲粮干物质消化率呈显著负相关(r=-0.51;P<0.05);3)猪空肠液消化酶活性与饲粮养分消化率间无典型相关关系(P>0.05),因此,脂肪酶活性对饲粮养分消化率变异的贡献有限。综合以上结果,生长猪空肠液消化酶活性与饲粮养分消化率间无稳定的相关性。

本文引用格式

王钰明, 赵峰, 廖睿, 张莉, 张虎, 张宏福 . 生长猪空肠液中主要消化酶活性与饲粮养分消化率的相关性研究[J]. 动物营养学报, 2015 , 27(10) : 3033 -3040 . DOI: 10.3969/j.issn.1006-267x.2015.10.007

Abstract

This experiment was conducted to investigate the relationship between digestive enzyme activity of jejunal fluid and dietary nutrient digestibility in pigs to provide reference for the study of nutrient digestion.Sixteen replicates per group were used in 2 groups, which were fed corn-soybean meal diet and corn-corn dry distillers dried grains with soluble (DDGS)-rice bran diet, respectively.The contents of dry matter (DM), crude protein (CP) and gross energy (GE) in the diets and feces, and digestive enzyme activity of jejunal fluid were determined.The relationship between digestive enzyme activity of jejunal fluid and dietary nutrient digestibility were calculated with simple correlation analysis and canonical correlation analysis.The results showed as follows: 1) the coefficient of variation (CV) of 4 digestive enzymes were over 20 % and CV of digestibility of DM, CP and GE were no more than 1.5 % in pigs fed both diets.2) No significant correlation were observed between the nutrient digestibility of diets and activity of amylase, trypsin or chymotrypsin (|r|≤0.51, P>0.05).Positive correlations were observed between the nutrient digestibility and activity of lipase in corn-soybean meal diet (r≥0.56, P<0.05).However, negative correlations were present between the DM digestibility and activity of lipase in corn-corn DDGS-rice bran diet (r=-0.51, P<0.05).3) No significant canonical correlations were observed between the digestive enzyme activity of jejunal fluid and nutrient digestibility of diets in pigs (P>0.05), so the contribution of lipase activity to the variation of nutrient digestibility was little.In conclusion, there are no stable correlation between the nutrient digestibility of diets and digestive enzyme activity of jejunal fluid in pigs.

参考文献

[1] 樊红平,侯水生,黄苇,等.鸡、鸭消化道pH和消化酶活的比较研究[J].畜牧兽医学报,2006,37(10):1009-1015.

[2] 王磊,王康宁.不同品种猪早期消化酶活性差异的研究[J].饲料工业,2008,29(8):58-61.

[3] CORRING T.The adaptation of digestive enzymes to the diet:its physiological significance[J].Reproduction Nutrition Développement,1980,20(4B):1217-1235.  

[4] ZEBROWSKA T,LOW A G.The influence of diets based on whole wheat,wheat flour and wheat bran on exocrine pancreatic secretion in pigs[J].The Journal of Nutrition,1987,117(7):1212-1216.

[5] HODGKINSON S M,MOUGHAN P J,REYNOLDS G W,et al.The effect of dietary peptide concentration on endogenous ileal amino acid loss in the growing pig[J].British Journal of Nutrition,2000,83(4):421-430.

[6] SOUFFRANT W B.Effect of dietary fibre on ileal digestibility and endogenous nitrogen losses in the pig[J].Animal Feed Science and Technology,2001,90(1/2):93-102.

[7] 胡光源,赵峰,张宏福,等.饲粮蛋白质来源与水平对生长猪空肠液组成的影响[J].动物营养学报,2010,22(5):1220-1225.

[8] DAHLQVIST A.A method for the determination of amylase in intestinal content[J].Scandinavian Journal of Clinical & Laboratory Investigation,1962,14(2):145-151.  

[9] WIRNT R.Trypsin,measurement with n-α-p-toluene-sulfonyl-l-arginine methyl ester as substrate[M]//Bergmeyer H U.Methods of Enzymatic Analysis.3rd ed.Weinheim,Germany:Verlag Chemie,1974:1021-1024.

[10] WIRNT R.Chymotrypsin,measurements with n-benzoyl-l-tyrosin methyl ester as substrate[M]//Bergmeyer H U.Methods of Enzymatic Analysis.3rd ed.Weinheim,Germany:Verlag Chemie,1974:1009-1012.

[11] KIM B G,LINDEMANN M D,CROMWELL G L,et al.The correlation between passage rate of digesta and dry matter digestibility in various stages of swine[J].Livestock Science,2007,109(1/2/3):81-84.

[12] 王钰明,赵峰,陈寿飞,等.猪生长阶段与饲粮类型对酸不溶灰分法测定养分消化率的影响[J].动物营养学报,2015,27(3):811-819.

[13] LEWIS A J,SOUTHERN L L.Swine nutrition[M].2nd ed.Boca Raton,London,New York,Washington,DC.:CRC Press,2001.

[14] HU C H,SONG J,YOU Z T,et al.Zinc oxide-montmorillonite hybrid influences diarrhea,intestinal mucosal integrity,and digestive enzyme activity in weaned pigs[J].Biological Trace Element Research,2012,149(2):190-196.  

[15] XU Y,SHI B,YAN S,et al.Effects of chitosan supplementation on the growth performance,nutrient digestibility,and digestive enzyme activity in weaned pigs[J].Czech Journal of Animal Science,2014,59(4):156-163.

[16] AUMAITRE A,CORRING T.Development of digestive enzymes in the piglet from birth to 8 weeks[J].Annals of Nutrition & Metabolism,1978,22(4):244-255.  

[17] MOUROT J,CORRING T,GUEUGNEAU A M,et al.Adaptation of the lipase-colipase system to dietary lipid content in pig pancreatic tissue[J].Annales de Biologie Animale Biochimie Biophysique,1979,19(1A):119-124.  

[18] 全国饲料工业标准化技术委员会.GB/T 26438—2010 畜禽饲料有效性与安全性评价全收粪法测定猪饲料表观消化能技术规程[S].北京:中国标准出版社,2011.

[19] PAREDES S P,JANSMAN A J M,VERSTEGEN M W A,et al.Identifying the limitations for growth in low performing piglets from birth until 10 weeks of age[J].Animal,2014,8(6):923-930.  

[20] MCCARTHY J F,AHERNE F X,OKAI D B.Use of HCl insoluble ash as an index material for determining apparent digestibility with pigs[J].Canadian Journal of Animal Science,1974,54(1):107-109.  

[21] OWSLEY W F,ORR D E,Jr.,TRIBBLE L F.Effects of age and diet on the development of the pancreas and the synthesis and secretion of pancreatic enzymes in the young pig[J].Journal of Animal Science,1986,63(2):497-504.

[22] KELLY D,SMYTH J A,MCCRACKEN K J.Digestive development of the early-weaned pig[J].British Journal of Nutrition,1991,65(2):169-180.  

[23] LOW A G.The activity of pepsin,chymotrypsin and trypsin during 24 h periods in the small intestine of growing pigs[J].British Journal of Nutrition,1982,48(1):147-159.  

[24] HORSZCZARUK F,BURACZEWSKA L,BURACZEWSKI S.Amount and composition of intestinal juice collected from isolated intestinal loops in pigs[J].Roczniki Nauk Rolniczych,1974,95(B-4):69-77.
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