本试验采用锰含量分别为3.45、8.26、15.98、23.16、28.67、33.83 mg/kg的等氮等能微颗粒饲料,对初始体重为(17.11±0.12) mg的方格星虫稚虫进行为期8周的饲养,用以研究饲料中锰含量对方格星虫稚虫生长性能、体成分、体腔液中锰超氧化物歧化酶活性及组织锰含量的影响。每种饲料设3个重复,每个重复饲喂400条方格星虫稚虫。结果表明:饲料中锰含量对方格星虫稚虫的增重率、特定生长率、成活率、虫体粗脂肪含量、虫体锰含量以及体腔液中锰超氧化物歧化酶(Mn-SOD)活性有显著影响(P<0.05),而对虫体水分、粗蛋白质、粗灰分以及体壁锰含量没有显著影响(P>0.05)。随着饲料中锰含量的增加,增重率、特定生长率、成活率、虫体粗脂肪含量以及体腔液中Mn-SOD活性均呈先升后降的趋势,当饲料锰含量为23.16 mg/kg时,增重率和特定生长率均达到最大值;成活率、虫体粗脂肪含量、体腔液中Mn-SOD活性则在饲料锰含量为28.67 mg/kg时达到最大值。以增重率为评价指标,通过回归方程得出方格星虫稚虫对饲料中锰的适宜需求量为22.58 mg/kg。
This trial was conducted to investigate the effects of dietary manganese content on growth performance, body composition, coelomic fluid manganese-superoxide dismutase (Mn-SOD) activity and tissue manganese content of juvenile peanut worm, Sipunculus nudus Linnaeus.Juvenile peanut worm with the average body weight of (17.11±0.12) mg were fed six isonitrogenous and isoenergetic microparticle diets with manganese level of 3.45, 8.26, 15.98, 23.16, 28.67, 33.83 mg/kg for 8 weeks, respectively.Each diet had three replicates and each replicate had 400 juvenile peanut worm.The results showed that dietary manganese content had significant effects on weight gain rate (WGR), specific growth rate (SGR), survival ratio (SR), body ether extract and manganese contents, and coelomic fluid Mn-SOD activity (P<0.05), but had no significant effects on body moisture, crude protein and ash, and body wall manganese contents (P>0.05).With the dietary manganese content increasing, the WGR, SGR, SR, body ether extract content and coelomic fluid Mn-SOD activity were firstly increased and then decreased.When the dietary manganese content was 23.16 mg/kg, WGR and SGR produced the maximum values, while SR, body ether extract content and coelomic fluid Mn-SOD activity had the maximum values when the dietary manganese content was 28.67 mg/kg.With WGR as the evaluating index, the suitable dietary manganese requirement of juvenile peanut worm is 22.58 mg/kg diet by regression equation.
[1] 陈细香,林秀雁,卢昌义,等.方格星虫属动物的研究进展[J].海洋科学,2008,32(6):66-70.
[2] 李凤鲁,孔庆兰,史贵田,等.中国沿海方格星虫属(星虫动物门)的研究[J].青岛海洋大学学报,1990,20(1):93-99.
[3] 张桂和,李理,赵谋明,等.方格星虫营养成分分析及抗疲劳作用研究[J].营养学报,2008,30(3):318-320.
[4] 张琴,童万平,董兰芳,等.饲料中脂肪水平对方格星虫稚虫生长性能、体组成及消化酶活性的影响[J].渔业科学进展,2011,32(6):99-106.
[5] 张琴,童万平,董兰芳,等.饲料蛋白水平对方格星虫稚虫生长和体组成的影响[J].渔业科学进展,2012,33(1):86-92.
[6] 张琴,童潼,童万平,等.饲料蛋白水平对方格星虫稚虫日增重和消化酶活性的影响[J].渔业现代化,2012,39(2):41-46.
[7] 许明珠,张琴,童万平,等.饲料糖水平对方格星虫稚虫生长、体组成和消化酶活性的影响[J].动物营养学报,2013,25(3):534-542.
[8] 许明珠,张琴,童潼,等.饲料铁水平对方格星虫稚虫生长性能、体成分、酶活性及组织铁含量的影响[J].动物营养学报,2014,26(11):3325-3331.
[9] BLAIR T,CASTELL J,NEIL S,et al.Evaluation of microdiets versus live feeds on growth,survival and fatty acid composition of larval haddock (Melanogrammus aeglefinus)[J].Aquaculture,2003,225(1/2/3/4):451-461.
[10] 倪可德.动物微量元素营养(一)[J].粮食与饲料工业,1996(2):28-32.
[11] 井明艳,孙建义,许梓荣.锰的生物学功能及有机态锰的应用研究[J].饲料博览,2004(1):7-9.
[12] 吴锐全,肖学铮,黄樟翰,等.鳗鲡的营养需求与饲料配制[J].饲料研究,1999(7):1-3.
[13] 王道尊,赵亮.一龄草鱼对锰的需要量[J].上海水产大学学报,1994(1):34-39.
[14] 黄耀桐,刘永坚.草鱼种无机盐需要量之研究[J].水生生物学报,1989,13(2):134-151.
[15] 李荷芳,王辉亮,梁海德,等.黑鲷对Ca,P,K,Mg和Mn的营养需求[J].海洋科学集刊,1996(37):135-141.
[16] 金明昌.斑点叉尾鮰营养需要研究进展[J].饲料研究,2008(3):56-58.
[17] 佐藤秀一.关于鱼类对微量元素利用特性的研究[J].饲料研究,1997(4):14-17.
[18] 董晓慧,周歧存,郑石轩,等.锰源和锰水平对南美白对虾生长性能和组织锰含量的影响[J].中国饲料,2005(9):29-31.
[19] 陈冬梅,刘维德.铜、铁、锌、锰不同组合水平对鲤鱼生长的影响[J].饲料工业,2003,24(4):50-52.
[20] 赵振山,徐毅刚,王寿兵,等.团头鲂配合饲料中锰和钴添加量的研究[J].水利渔业,1995(1):22-24.
[21] 蒋蓉.铜、铁、锰、锌对黄颡鱼生长和生理机能的影响[D].硕士学位论文.苏州:苏州大学,2006.
[22] 魏万权,李爱杰,李德尚.牙鲆幼鱼饲料中锰、钴适宜添加量的初步研究[J].浙江海洋学院学报:自然科学版,2001,20(增刊):83-87.
[23] ROMANENKO V D.Metabolic aspects of warmwater culture of carp,Cyprinus carpio L.[J].Aquaculture,38(4):307-314.
[24] 袁建明.饲料Cu、Mn补充量对异育银鲫生长、部分生理机能及器官中微量元素含量的影响[D].硕士学位论文.苏州:苏州大学,2008.
[25] 张金环,甄二英,张燕铭,等.锰的营养学研究进展[J].饲料博览,2005(2):8-11.
[26] 王宏伟,曹向可,钱庆增,等.饲料中锰对日本沼虾抗氧化酶活性的影响[J].河北大学学报:自然科学版,2008,28(3):300-304.
[27] 王宏伟,郭立格,赵建华,等.饲料中锰对中华米虾SOD活性的影响[J].河北大学学报:自然科学版,2006,26(6):649-652.
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