饲料营养 Feed Science and Technology

N-氨甲酰谷氨酸与牛磺酸对公猪精液品质、血清激素指标及精浆抗氧化能力的影响

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  • 1. 沈阳农业大学畜牧兽医学院, 沈阳 110866;
    2. 辽宁德宝农牧集团有限公司, 沈阳 110171;
    3. 沈阳福康农牧科技有限公司, 沈阳 110164
朱宇旌(1972—),女,辽宁彰武人,博士,副教授,主要从事动物营养与饲料科学研究。E-mail:syndzhyj@163.com

收稿日期: 2015-04-15

  网络出版日期: 2015-10-15

基金资助

国家自然科学基金(31440082,31101253)

Effects of N-Carbamyl Glutamate and Taurine on Semen Quality, Serum Hormone Parameters and Antioxidant Capacity of Breeding Boars

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  • 1. College of Animal Sciences and Veterinary Sciences, Shenyang Agricultural University, Shenyang 110866, China;
    2. Liaoning Debao Argi-Animal Husbandry Co., Ltd., Shenyang 110171, China;
    3. Shenyang Fukang Agriculture and Animal Husbandry Technology Co., Ltd., Shenyang 110164, China

Received date: 2015-04-15

  Online published: 2015-10-15

摘要

本试验旨在研究饲粮添加N-氨甲酰谷氨酸(NCG)与牛磺酸(Tau)对公猪精液品质、血清激素指标、精浆蛋白含量及抗氧化能力的影响。选取18月龄、体重相近的健康大约克公猪20头,随机分为4组,每组5个重复,每个重复1头猪。各组分别饲喂基础饲粮(对照组)、基础饲粮+0.2%NCG(NCG组)、基础饲粮+0.6%牛磺酸(Tau组)、基础饲粮+0.2%NCG+0.6%牛磺酸(NCG+Tau组)。试验期100 d。结果表明:1)试验56~100 d,与对照组比较,饲粮中添加NCG、Tau及NCG+Tau公猪射精反应时间极显著降低(P<0.01),射精持续时间极显著提高(P<0.01)。主效应分析结果表明,试验56~100 d,饲粮中添加NCG可显著提高采精量(P<0.05),饲粮中添加Tau或Tau+NCG可显著提高精子活力(P<0.05),饲粮中添加NCG可显著降低精子畸形率(P<0.05);2)试验56~100 d,与对照组比较,饲粮中添加NCG或NCG+Tau可极显著提高血清促卵泡素(FSH)含量(P<0.01),饲粮中添加NCG+Tau可极显著提高血清黄体生成素(LH)和睾酮(T)含量(P<0.01)。3)主效应分析结果表明,饲粮中添加NCG后精浆总蛋白(TP)含量显著提高(P<0.05),饲粮中添加Tau后精浆白蛋白(ALB)含量显著提高(P<0.05)。4)主效应分析结果表明,试验56~100 d,饲粮中添加NCG可显著提高精浆总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05),饲粮中添加NCG或Tau后精浆超氧化物歧化酶(SOD)活性显著提高(P<0.05)。综合分析,饲粮中添加NCG与Tau可调控公猪血清激素分泌水平,提升性欲,提高精浆蛋白含量和抗氧化能力,从而提高精液品质。

本文引用格式

朱宇旌, 朱广楠, 李方方, 郑丽莉, 高原, 孟玲, 郭福来, 丁兰, 米春桃, 张勇 . N-氨甲酰谷氨酸与牛磺酸对公猪精液品质、血清激素指标及精浆抗氧化能力的影响[J]. 动物营养学报, 2015 , 27(10) : 3125 -3133 . DOI: 10.3969/j.issn.1006-267x.2015.10.018

Abstract

This study was conducted to determine the effects of dietary supplementation of N-carbamyl glutamate (NCG) and taurine (Tau) on semen quality, serum hormone parameters, seminal plasma protein content and antioxidant capacity of breeding boars.Twenty 18-month-old Yorkshire boars with similar weight healthy were randomly divided into 4 groups with 5 replicates per group and 1 pig per replicate.Pigs were fed basal diet (control group), basal diet+0.2% NCG (NCG group), basal diet+0.6% Tau (Tau group), basal diet+0.2% NCG+0.6% Tau (NCG+Tau group).The trial lasted for 100 days.The results showed as follows: 1) compared with the control group, dietary supplemented with NCG, Tau and NCG+Tau significantly decreased the ejaculation reaction time (P<0.01), and significantly increased the ejaculation time (P<0.01).The main effects analytic result showed that during 56 to 100 days, dietary supplemented with NCG significantly improved the semen volume (P<0.05), dietary supplemented with NCG or Tau+NCG significantly improved the sperm motility (P<0.05), dietary supplemented with NCG significantly decreased the percentage of abnormal sperm (P<0.05).2) Compared with the control group, during 56 to 100 days, dietary supplemented with NCG or NCG+Tau significantly improved serum follicle stimulating hormone (FSH) content (P<0.01), dietary supplemented with NCG+Tau significantly improved the content of luteinizing hormone (LH) and testosterone (T) in serum (P<0.01).3) The main effects analytic result showed that dietary supplemented with NCG significantly improved seminal plasma total protein (TP) content (P<0.05), dietary supplemented with Tau significantly improved seminal plasma albumin (ALB) content (P<0.05).4) The main effects analytic result showed that during 56 to 100 days, dietary supplemented with NCG significantly improved total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activity in seminal plasma (P<0.05), dietary supplemented with Tau or NCG+Tau significantly improved seminal plasma superoxide dismutase (SOD) activity (P<0.05).Analyze the data synthetically, dietary supplemented with NCG and Tau can regulate the serum hormone secretion level, help to increase the sexual desire, and improve the protein quality in seminal plasma, as well as the antioxidant capacity and the quality of semen.

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