不同微生态制剂对蛋鸡机体超氧化物歧化酶调节机制的影响

张鑫, 金锡九, 金海国, 张敏

doi:10.3969/j.issn.1006-267x.2015.10.019

动物营养学报 >

2015 , Vol. 27 >Issue 10: 3134 - 3139

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.10.019

饲料营养 Feed Science and Technology

不同微生态制剂对蛋鸡机体超氧化物歧化酶调节机制的影响

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1. 延边大学农学院, 延吉 133002;
2. 吉林省农业科学院, 公主岭 136100

张鑫(1989—),女,硕士研究生,黑龙江大庆人,从事动物营养与饲料科学研究。E-mail:809517162@qq.com

收稿日期: 2015-04-14

网络出版日期: 2015-10-15

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Effects of Different Kinds of Probiotics on Superoxide Dismutase Regulatory Mechanism of Laying Hens

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1. Department of Animal Science, Yanbian University, Yanji 133002, China;
2. Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China

Received date: 2015-04-14

Online published: 2015-10-15

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摘要

本试验旨在研究不同微生态制剂对蛋鸡机体超氧化物歧化酶(SOD)调节机制的影响。选择360只健康的海兰褐蛋鸡,随机分成5组,每组3个重复,每个重复24只。对照组(Ⅰ组)饲喂基础饲粮,试验组(Ⅱ、Ⅲ、Ⅳ、Ⅴ组)分别在基础饲粮中添加0.5%中药乳酸菌、0.25%乳酸菌制剂+0.25%中药红曲霉制剂、0.5%乳酸菌制剂和0.5%中药红曲霉制剂。试验期为79 d。结果表明:1)与对照组相比,试验组中蛋鸡血清总超氧化物歧化酶(T-SOD)活性显著提高(P<0.05),Ⅱ、Ⅲ和Ⅳ组蛋黄中T-SOD活性显著提高(P<0.05)。2)与对照组相比,Ⅱ组蛋鸡肝脏中SOD1 mRNA的表达量显著提高(P<0.05),Ⅱ、Ⅳ和Ⅴ组的蛋鸡肝脏中SOD2 mRNA表达量显著提高(P<0.05)。由此可见,饲粮中添加上述微生态制剂能调节蛋鸡机体SOD机制,以添加0.5%中药乳酸菌制剂效果最好。

关键词： 微生态制剂; SOD; mRNA; 蛋鸡

本文引用格式

张鑫, 金锡九, 金海国, 张敏 . 不同微生态制剂对蛋鸡机体超氧化物歧化酶调节机制的影响[J]. 动物营养学报, 2015 , 27(10) : 3134 -3139 . DOI: 10.3969/j.issn.1006-267x.2015.10.019

Abstract

This experiment was conducted to investigate the effects of different kinds of probiotics on superoxide dismutase (SOD) regulatory mechanism of laying hens.A total of 360 healthy Hyline laying hens were randomly divided into 5 groups with 3 replicates per group and 24 hens per replicate.The hens in control group (group Ⅰ) were fed a basal diet, and the others in experimental groups (groupsⅡ, Ⅲ, Ⅳ and Ⅴ) were fed the basal diets supplemented with 0.5% preparation of lactic acid bacteria with traditional Chinese medicine, 0.25% lactic acid bacteria preparation mixed with 0.25% Monascus preparations with traditional Chinese medicine, 0.5% lactic acid bacteria preparation and 0.5% Monascus preparations with traditional Chinese medicine, respectively.The experiment lasted for 79 days.The results showed as follows: 1)compared with the group Ⅰ, the serum total superoxide dismutase (T-SOD) activity of experimental groups was significantly increased (P<0.05), the yolk T-SOD activity of groups Ⅱ, Ⅲand Ⅳ was significantly increased (P<0.05).2) Compared with the group Ⅰ, the liver SOD1 mRNA expression of group Ⅱ was significantly increased (P<0.05), the liver SOD2 mRNA expression of group Ⅱ, Ⅳ and Ⅴ was significantly increased (P<0.05).In conclusion, different kinds of probiotics can regulate the mechanism on SOD of laying hens, and diets supplemented with 0.5% preparation of lactic acid bacteria with traditional Chinese medicine has the best result in this experiment.

Key words： probiotics; SOD; mRNA; laying hens

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