饲料营养 Feed Science and Technology

不同剂型丁酸钠对脂多糖应激肉鸡血清生化指标、抗氧化和抗炎功能的影响

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  • 浙江大学动物科学学院, 杭州 310058
鞠婷婷(1990—),女,山东潍坊人,硕士研究生,从事动物营养与调控研究。E-mail:Jutingting1@163.com

收稿日期: 2015-04-14

  网络出版日期: 2015-10-15

基金资助

国家肉鸡产业技术体系(CARS-42-G19);浙江省重大科技专项(2013C02010)

Effects of Different Type of Sodium Butyrate on Serum Biochemical Indices, Antioxidant and Anti-Inflammatory Function of Broilers Challenged with Lipopolysaccharide

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  • College of Animal Science, Zhejiang University, Hangzhou 310058, China

Received date: 2015-04-14

  Online published: 2015-10-15

摘要

本试验旨在研究不同剂型丁酸钠对脂多糖(LPS)应激肉鸡血清生化指标、抗氧化和抗炎功能的影响。饲养试验将1 000羽1日龄岭南黄肉鸡随机分为5组,每组4个重复,每重复50羽。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加500 mg/kg粉剂丁酸钠(NaB)及250、500、750 mg/kg包膜丁酸钠(CNaB),试验为期50 d。应激试验分别从对照组、NaB组以及生长性能最佳组(250 mg/kg CNaB组),每重复随机选6只鸡,按2×3因子试验设计,以应激种类(生理盐水和LPS)和饲粮类型(0、500 mg/kg NaB和250 mg/kg CNaB)作为主效因子,各组1/2鸡只于53、56和59 d腹腔注射大肠杆菌LPS(0.5 mg/kg BW),剩余鸡只则注射等量生理盐水,试验为期10 d。结果表明:1)与生理盐水注射组相比,LPS注射组显著降低了血清中葡萄糖和甘油三酯含量(P<0.05),显著提高了血清尿酸含量(P<0.05)。LPS注射下,NaB组和CNaB组中血清葡萄糖含量均显著高于对照组(P<0.05)。2)与生理盐水注射组相比,LPS注射组显著或极显著降低了血清和肝脏中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05或P<0.01),显著降低了血清过氧化氢酶(CAT)活性(P<0.05);LPS注射下,NaB组和CNaB组血清和肝脏SOD、CAT活性均高于对照组(P>0.05),丙二醛(MDA)含量低于对照组(P>0.05)。LPS注射和饲粮添加丁酸钠对肝脏GSH-Px活性影响存在交互作用(P<0.05),且2种剂型作用效果相类似。3)与生理盐水注射组相比,LPS注射组极显著提高53和59 d血清铜蓝蛋白和白细胞介素-6(IL-6)含量(P<0.01);LPS注射下,NaB组和CNaB组53 d血清铜蓝蛋白含量显著低于对照组(P<0.05)。由此可见,在LPS应激条件下,丁酸钠能改善肉鸡营养代谢,维持机体抗氧化功能,增强抗炎功能,且500 mg/kg NaB和250 mg/kg CNaB(有效含量为75 mg/kg)作用效果基本一致。

本文引用格式

鞠婷婷, 郭孝烨, 随佳佳, 肖雪, 占秀安 . 不同剂型丁酸钠对脂多糖应激肉鸡血清生化指标、抗氧化和抗炎功能的影响[J]. 动物营养学报, 2015 , 27(10) : 3146 -3154 . DOI: 10.3969/j.issn.1006-267x.2015.10.021

Abstract

The trial was conducted to study on the effects of different type of sodium butyrate on serum biochemical indices, antioxidant and anti-inflammatory function of broilers challenged with lipopolysaccharide (LPS).Feeding trial: a total of 1 000 one-day-old Lingnan yellow broilers were randomly allocated into 5 groups with 4 replicates in each group and 50 broilers per replicate.Broilers in control group were fed a basal diet, the others in experimental groups were fed the basal diets supplemented with 500 mg/kg powder sodium butyrate (NaB), and 250, 500 and 750 mg/kg coated sodium butyrate (CNaB), respectively.The LPS injection experiment was conducted followed a 50-day feeding trial, 72 fifty-day-old female broilers were selected randomly from control, NaB and 250 mg/kg CNaB group with the best growth performance, with 6 broilers per replicate.And they were allotted into a 2×3 factorial arrangement with challenge status (saline or LPS exposure) and feed diet (0, 500 mg/kg NaB or 250 mg/kg CNaB) as main factor for endotoxin exposure test.Half of broilers in each group were injected intraperitoneally with E.coli LPS (0.5 mg/kg body weight)at 53, 56 and 59 d of age, the remaining broilers were injected intraperitoneally with the same amount of saline as the LPS injected group.The trial lasted for 10 days.The results showed as follows: 1) compared with the saline injected group, the content of glucose and riglyceride in serum was significantly decreased in LPS injected group (P<0.05), but the serum uric acid content as significantly increased (P<0.05).The serum glucose content of NaB and CNaB groups injected with LPS was significantly higher than that of control group (P<0.05).2) Compared with the saline injected group, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum and liver was significantly decreased in LPS injected group (P<0.05 or P<0.01), the serum catalase (CAT) activity was significantly decreased (P<0.05).The activity of SOD and CAT in serum and liver of NaB and CNaB groups injected with LPS was higher than that of control group (P>0.05), but the malonaldehyde content was lower than that of control group (P>0.05).There were a significant interaction between dietary sodium butyrate and LPS injected on liver GSH-Px activity (P<0.05), and the two forms of sodium butyrate exerted an equal effect on antioxidant enzyme activity.3) Compared with the saline injected group, the content of ceruloplasmin and interleukin-6 in serum at the age of 53 and 59 days was significantly increased in LPS injected group (P<0.05).The serum ceruloplasmin content at the age of 53 days of NaB and CNaB groups injected with LPS was significantly lower than that of control group (P<0.05).In conclusion, sodium butyrate can improve the nutrient metabolism, maintain antioxidant function and promote anti-inflammation function, the effects on anti-stress of 500 mg/kg NaB are equal to those of 250 mg/kg CNaB which contains 75 mg/kg effective substance.

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