饲料安全 Feed Security

饲粮游离棉酚水平对蛋鸡肝脏棉酚蓄积及生产性能、蛋品质的影响

展开
  • 山东农业大学动物科技学院, 泰安 271000
焦洪超(1977—),男,山东临沂人,博士,研究方向为动物营养与环境。E-mail:hongchao@sdau.edu.cn

收稿日期: 2015-07-27

  网络出版日期: 2015-10-15

基金资助

国家自然科学基金项目(31201816);现代农业产业技术体系建设专项资金项目(CARS-41-K14)

Effects of Dietary Free Gossypol Levels on Liver Gossypol Accumulation, Performance and Egg Quality of Laying Hens

Expand
  • College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China

Received date: 2015-07-27

  Online published: 2015-10-15

摘要

本试验旨在探讨含游离棉酚饲粮长期饲喂蛋鸡的肝脏中棉酚蓄积规律及其对蛋鸡生产性能和蛋品质的影响,为更好地利用棉籽粕资源提供理论支持。选取23周龄海兰褐蛋鸡270只,随机分为3组,饲粮中游离棉酚水平分别为0、20和40 mg/kg,试验期45周。结果表明:长期饲喂含游离棉酚饲粮造成了蛋鸡肝脏中游离棉酚的蓄积,且呈剂量效应,引起肝细胞结构紊乱和损伤,不同游离棉酚水平饲粮长期饲喂未引起蛋鸡生产性能和产蛋性能的显著性变化(P>0.05),但产蛋后期(31~45周)蛋鸡死淘率显著升高(P<0.05),而且1~10周蛋黄颜色显著增大(P<0.05)。由此可见,含游离棉酚饲粮长期饲喂蛋鸡造成其肝脏中游离棉酚的蓄积,进而影响其肝脏功能,饲粮游离棉酚含量为20和40 mg/kg时对蛋鸡生产性能影响较小,但加重了产蛋后期蛋鸡死淘率。结果提示,蛋鸡死淘率的增加可能源于棉酚蓄积毒性造成的肝脏损伤。

本文引用格式

焦洪超, 侯楠楠, 姜明君, 宋志刚, 林海 . 饲粮游离棉酚水平对蛋鸡肝脏棉酚蓄积及生产性能、蛋品质的影响[J]. 动物营养学报, 2015 , 27(10) : 3207 -3217 . DOI: 10.3969/j.issn.1006-267x.2015.10.028

Abstract

This experiment was conducted to investigate the effects of hepatic gossypol accumulation and the performance and egg quality of laying hens which long-term feeding with diet containing free gossypol, in order to provide theoretical support to make better use of cotton meal resources.Two hundred and seventy Hy-Line brown laying hens of 23-week-old were randomly subjected to three groups for 45 weeks, the content of free gossypol in three diets was 0, 20 and 40 mg/kg, respectively.The results showed that laying hens fed with free gossypol diet for long-term induced the accumulation of free gossypol in the liver, lead to disorder and injury of hepatic cell structure.Compared with the control group, long-term fed with free gossypol diets had no significantly effect on performance and laying performance of laying hens (P>0.05), but significantly increased the mortality and elimination rate at later stage (from 31 to 45 weeks) and increased yolk color from 1 to 10 weeks (P<0.05).It is concluded that long-term feeding with gossypol diet can result in accumulation of free gossypol in hens' liver, and then the gossypol toxicity induce liver injury and affect liver function.Although diets containing 20 or 40 mg/kg free gossypol have little effect on the performance and laying performance of laying hens, but the mortality and elimination rate at later stage of hens are significant high.Results indicate that the higher mortality and elimination rate of hens may be connected with the cumulative toxicity of gossypol in liver.

参考文献

[1] FRANCIS G,MAKKAR H P S,BECKER K.Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish[J].Aquaculture,2001,199(3/4):197-227.

[2] ROBINSON P H,GETACHEW G,DE PETERS E J,et al.Influence of variety and storage for up to 22 days on nutrient composition and gossypol level of Pima cottonseed (Gossypium spp.)[J].Animal Feed Science and Technology,2001,91(3/4):149-156.

[3] BARRAZA M L,CORROEK C E,BROOKS K N,et al.Iron sulfate and feed pelleting to detoxify free gossypol in cottonseed diets for dairy cattle[J].Journal of Dairy Science,1991,74(10):3457-3467.  

[4] ZINN R A,MONTAÑO M,ALVAREZ E,et al.Feeding value of cottonseed meal for feedlot cattle[J].Journal of Animal Science,1997,75(9):2317-2322.

[5] 何国茹.玉米、豆粕、棉粕、玉米蛋白粉主要营养成分含量变化规律分析[D].硕士学位论文.杨凌:西北农林科技大学,2011:30-31.

[6] BARAM N I,ISMAILOV A I.Biological activity of gossypol and its derivatives[J].Chemistry of Natural Compounds,1993,29(3):275-287.  

[7] CALHOUN M C,KUHLMANN S W,BALDWIN B C.Assessing the gossypol status of cattle fed cottonseed products[C]//Proceedings of the Pacific Northwest Animal Nutrition Conference.Portland,O.R.:[s.n.],1995:147A-157A.

[8] BLACKWELDER J T,HOPKINS B A,DIAZ D E,et al.Milk production and plasma gossypol of cows fed cottonseed and oilseed meals with or without rumen-undegradable protein[J].Journal of Dairy Science,1998,81(11):2934-2941.  

[9] NOFTSGER S M,HOPKINSB A,DIAZ D E,et al.Effect of whole and expanded-expelled cotton seed on milk yield and blood gossypol[J].Journal of Dairy Science,2000,83(11):2539-2547.  

[10] ABOU-DONIA M B,LYMAN C M,DIECDKERT J W.Metabolic fate of gossypol:the metabolism of 14C-gossypol in rats[J].Lipids,1970,5(11):938-946.  

[11] REKHA G K,SLADEK N E.Inhibition of human class 3 aldehyde dehydrogenase,and sensitization of tumor cells that express significant amounts of this enzyme to oxazaphosphorines,by the naturally occurring compound gossypol[M]//WEINER H,LINDAHL R,CRABB D W,et al.Enzymology and molecular biology of carbonyl metabolism 6.New York:Plenum Press,1997,414:133-146.

[12] SHELLEY M D,HARTLEY L,FISH R G,et al.Stereo-specific cytotoxic effects of gossypol enantiomers and gossypolone in tumour cell lines[J].Cancer Letters,1999,135(2):171-180.  

[13] KIM H L,CALHOUN M C.Determination of gossypol in plasma and tissues of animals[J].Inform,1995,6(4):486.

[14] HRON R J,KUK M S,ABRAHAM G.Determination of free and total gossypol by high performance liquid chromatography[J].Journal of the American Oil Chemists' Society,1990,67(3):182-187.  

[15] CHEN Q Q,CHEN H,LEI H P.Comparative study on the metabolism of optical gossypol in rats[J].Journal of Ethnopharmacology,1987,20(1):31-37.  

[16] LORDELO M M,CALHOUN M C,DALE N M,et al.Relative Toxicity of gossypol enantiomers in laying and broiler breeder hens[J].Poultry Science,2007,86(3):582-590.  

[17] SHARMA M P,SMITH F H,CLAWSON A J.Effects of levels of protein and gossypol,and length of feeding period on the accumulation of gossypol in tissues of swine[J].Journal of Nutrition,1966,88(4):434-438.

[18] MORGAN S E.Gossypol residues in organ meats vs thresholds of toxicity[J].Veterinary and Human Toxicology,1990,32(Suppl.):76-80.

[19] GAMBOA D A,CALHOUN M C,KUHLMANN S W,et al.Tissue distribution of gossypol enantiomers in broilers fed various cottonseed meals[J].Poultry Science,2001,80(7):920-925.  

[20] LORDELO M M,DAVIS A J,CALHOUN M C,et al.Relative toxicity of gossypol enantiomers in broilers[J].Poultry Science,2005,84(9):1376-1382.  

[21] 张志峰,焦海宏,俞华英,等.育肥猪棉酚中毒的病理形态学观察与分析[J].中国兽医杂志,2011,47(4):52-53.

[22] 庄元忠,徐敏源,赵秀菊,等.棉酚对猕猴肝肾影响的电镜初步观察[J].浙江医学,1981,3(1):27.

[23] 周兰芳,王英,李娟,等.醋酸棉酚对大鼠肝脏影响的组织化学及电镜观察[J].中国医学科学院学报,1988(5):385.

[24] 冷青文,申红,王志祥.未脱毒棉籽饼对蛋鸡生产性能及其生殖器官的影响[J].畜禽业,1999(1):34-35.

[25] 李建国,王建华.游离棉酚水平对海兰蛋鸡生产性能的影响[J].家畜生态学报,2007,28(6):31-33.

[26] PANIGRAHI S,PLUMB V E,MACHIN D H.Effects of dietary cottonseed meal,with and without iron treatment,on laying hens[J].British Poultry Science,1989,30(3):641-651.  

[27] 方琴音.饲粮不同棉籽饼用量对商品蛋鸡生产性能、蛋品质、血液生化指标的影响研究[D].硕士学位论文.雅安:四川农业大学,2004:17-18.

[28] 杨茹洁.可消化AA平衡的高棉粕饲粮对蛋鸡的生产性能、健康状况及蛋品质的影响[D].硕士学位论文.太谷:山西农业大学,2003:12-14.

[29] 陈连颐,唐梦君,陆俊贤,等.棉籽粕对海赛克斯蛋鸡生产性能及蛋品质的影响[J].中国家禽,2012,34(24):29-32.

[30] GILANI A,KERMANSHAHI H,GOLIAN A,et al.Impact of sodium bentonite addition to the diets containing cottonseed meal on productive traits of HY-line W-36 hens[J].The Journal of Animal and Plant Sciences,2013,23(2):411-415.

[31] 尹进,王美岭,徐贝力,等.棉粕饲料导致鸡蛋变色的机理探讨[J].中国粮油学报,1992,7(3):57-60.
文章导航

/