饲料维生素C含量对半滑舌鳎抵抗迟缓爱德华氏菌感染的影响

黄亚冬, 孙朦朦, 窦艳君, 王庆奎, 陈成勋, 邢克智

doi:10.3969/j.issn.1006-267x.2015.10.035

动物营养学报 >

2015 , Vol. 27 >Issue 10: 3263 - 3269

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.10.035

研究简报 Short Communications

饲料维生素C含量对半滑舌鳎抵抗迟缓爱德华氏菌感染的影响

展开

1. 天津海升水产养殖有限公司, 天津 300272;
2. 天津海友佳音生物科技股份有限公司, 天津 300356;
3. 天津农学院水产科学系, 天津市水产生态及养殖重点实验室, 天津 300384

黄亚冬(1986—),男,河南漯河人,硕士研究生,从事水产养殖研究。E-mail:yadong6666@126.com

收稿日期: 2015-04-28

网络出版日期: 2015-10-15

基金资助

"十二五"国家科技支撑计划(2011BAD13B07);国家星火重大项目(2013GA610002);天津市高等学校创新团队基金项目(TD12-5018)

收起

Effects of Dietary Vitamin C on Resistance in Edwardsiella tarda Infection of Tongue Sole (Cynoglossus semilaevis)

Expand

1. Tianjin Haisheng Aquaculture Co., Ltd., Tianjin 300272, China;
2. Tianjin Ocean Pal Carol Biotech Co., Ltd., Tianjin 300356, China;
3. Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, Department of Fishery Science, Tianjin 300384, China

Received date: 2015-04-28

Online published: 2015-10-15

Fold

摘要

本试验旨在研究维生素C对半滑舌鳎在感染迟缓爱德华氏菌后红细胞抗氧化能力及葡萄糖-6-磷酸脱氢酶(G-6-PD)活性的影响。在基础饲料中添加L-抗坏血酸-2-磷酸酯(作为维生素C添加剂),制成维生素C含量分别为0.2(对照)、104.8、209.6、313.8、521.6、837.3 mg/kg的6种试验饲料。将健康的半滑舌鳎[平均体重(99.12±1.58) g]分别喂食上述6种饲料84 d后,感染迟缓爱德华氏菌,试验期为14 d。结果显示:试验鱼的终末体重、特定生长率、增重率均以209.6 mg/kg组最高。随着饲料中维生素C含量的升高,半滑舌鳎血浆维生素C含量及红细胞血红蛋白(Hb)含量和G-6-PD活性均呈上升趋势,且除104.8 mg/kg组红细胞G-6-PD活性外各添加组均显著高于对照组(P<0.05)。饲料维生素C含量为209.6 mg/kg时,红细胞超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著高于对照组(P<0.05),而后随着维生素C含量继续升高,红细胞SOD和CAT活性呈下降趋势。对照组红细胞丙二醛(MDA)含量和红细胞膜一氧化氮(NO)含量显著高于各添加组(P<0.05)。以上结果表明,维生素C能有效降低感染迟缓爱德华氏菌半滑舌鳎的红细胞氧化损伤程度;经过折线模型分析,推荐饲料中维生素C含量为257.04~341.36 mg/kg。

关键词： 半滑舌鳎; 维生素C; 迟缓爱德华氏菌; 红细胞; 红细胞膜; 抗氧化能力

本文引用格式

黄亚冬, 孙朦朦, 窦艳君, 王庆奎, 陈成勋, 邢克智 . 饲料维生素C含量对半滑舌鳎抵抗迟缓爱德华氏菌感染的影响[J]. 动物营养学报, 2015 , 27(10) : 3263 -3269 . DOI: 10.3969/j.issn.1006-267x.2015.10.035

Abstract

This study was conducted to investigate the effects of vitamin C on red blood cell (RBC) antioxidation capacity and glucose-6-phosphate (G-6-PD) activity of tongue sole (Cynoglossus semilaevis).Six experimental diets were prepared with vitamin C content of 0.2 (control), 104.8, 209.6, 313.8, 521.6 and 837.3 mg/kg in form of L-ascorbyl-2-polyphosphate, respectively, and those diets were fed healthy tongue sole [average body weight of (99.12±1.58) g] for 84 days.After feeding trial, The test fish were challenged with Edwardsiella tarda for 14 days.The results showed as follows: the highest values of final body weight, special growth rate and weight gain rate were found in the 209.6 mg/kg group.With the dietary vitamin C content increasing, the vitamin C content in plasma, and hemoglobin (Hb) content and G-6-PD activity in RBC all showed an increasing tendency, and all additional groups were all significantly higher than the control group (P<0.05) except RBC G-6-PD activity in 104.8 mg/kg group.The SOD and CAT activities in RBC in 209.6 mg/kg group were significantly higher than those in the control group (P<0.05), and then the activities decreased with continued to increase the dietary vitamin C content.The malondialdehvde (MDA) content in RBC and nitric oxide (NO) in RBC membrane in the control group were significantly higher than those in all additional groups (P<0.05).The results indicate that the vitamin C can effectively reduce the oxidant damage of RBC of tongue sole with Edwardsiella tarda infection.The suitable dietary vitamin C content is 257.04 to 341.36 mg/kg which estimated by broken-line models.

Key words： tongue sole (Cynoglossus semilaevis); vitamin C; Edwardsiella tarda; RBC; RBC membrane; antioxidation capacity

参考文献

[1] 莫简.医用自由基生物学导论[M].北京:人民卫生出版社,1989.

[2] 海春旭.自由基医学[M].西安:第四军医大学出版社,2006.

[3] FARHUD D D,YAZDANPANAH L.Glucose-6-phosphate dehydrogenase (G6PD) deficiency[J].Iranian Journal of Public Health,2008,37(4):1-18.

[4] 陈晓军.红细胞G6PD速率法检测及对贫血患者的诊断意义[J].中国实验诊断学,2006,10(7):773.

[5] 詹彤,陶靖,唐荣才,等.维生素C对人全血红细胞的抗氧化保护作用[J].中国实验血液学杂志,2005,13(6):1106-1108.

[6] 孙永叶,马爱国,张秀珍,等.维生素C缺乏对豚鼠抗氧化能力及红细胞膜流动性的影响[J].卫生研究,2005,34(3):329-330.

[7] 薛美兰,马爱国,张秀珍.大剂量维生素C对大鼠抗氧化能力及淋巴细胞增殖功能影响的研究[J].营养学报,2008,30(5):525-527.

[8] PIMPIMOL T,PHOONSAMRAN K,CHITMANAT C.Effect of dietary vitamin c supplementation on the blood parameters of Mekong giant catfish (Pangasianodon gigas)[J].International Journal of Agriculture and Biology,2012,14(2):256-260.

[9] IBRAHEM M D,FATHI M,MESALHY S,et al.Effect of dietary supplementation of inulin and vitamin C on the growth,hematology,innate immunity,and resistance of Nile tilapia (Oreochromis niloticus)[J].Fish & Shellfish Immunology,2010,29(2):241-246.

[10] 朱国霞,黄亚冬,孙朦朦,等.维生素E对半滑舌鳎抗迟缓爱德华氏菌感染的影响[J].饲料研究,2013(9):1-4.

[11] 封家旺.维生素E缺乏对雏鸡红细胞膜的影响[D].硕士学位论文.哈尔滨:东北农业大学,2008.

[12] BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantifies of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72:248-254.

[13] 封家旺,李艳飞,张金龙,等.维生素E缺乏对雏鸡红细胞抗氧化性能及G-6-PD活性的影响[J].中国家禽,2007,29(14):43-44,57.

[14] 严建伟,阮积慧.生物体系中自由基检测方法评述[J].杭州大学学报:自然科学版,1998,25(3):75-80.

[15] EISENSTEIN T K,HUANG D,MEISSLER J J,et al.Macrophage nitric oxide mediates immunosuppression in infectious inflammation [J].Immunobiology,1994,191(4/5):493-502.

[16] FARUQI R,DE LA MOTTE C,DICORLETO P E.Alpha-tocopherol inhibits agonist-induced monocytic cell adhesion to cultured human endothelial cells[J].Journal of Clinical Investigation,1994,94:592-600.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们