综述 Review

鱼类钠离子和氯离子转运载体的功能及调控机制研究进展

  • 吉中力 ,
  • 张春晓 ,
  • 麦康森
展开
  • 1. 集美大学, 农业部东海海水健康养殖重点实验室, 厦门 361021;
    2. 集美大学, 厦门市饲料检测与安全评价重点实验室, 厦门 361021;
    3. 中国海洋大学, 水产动物营养与饲料农业部重点实验室, 青岛 266003

收稿日期: 2015-08-24

  网络出版日期: 2016-02-19

基金资助

国家自然科学基金(31001115);福建省高校优秀人才支持计划(JA11145)

A Review on Function and Regulatory Mechanism of Na+ and Cl- Transporters in Fish

  • JI Zhongli ,
  • ZHANG Chunxiao ,
  • MAI Kangsen
Expand
  • 1. The Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen 361021, China;
    2. Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation, Jimei University, Xiamen 361021, China;
    3. The Key Laboratory of Mariculture, Education Ministry of China, Ocean University of China, Qingdao 266003, China

Received date: 2015-08-24

  Online published: 2016-02-19

摘要

钠离子(Na+)和氯离子(Cl-)不仅参与鱼类体液的渗透压平衡调节,也参与细胞膜静息电位平衡调节,并且鱼类机体内部电解质的稳态也离不开Na+和Cl-的参与。位于硬骨鱼类鳃、胃肠道以及肾小管上皮细胞膜上的Na+/钾离子(K+)-ATP酶、Na+-K+-2Cl-协同转运蛋白、Na+/氢离子(H+)交换蛋白、囊性纤维化跨膜调控子等相关载体蛋白,是鱼类调控Na+和Cl-代谢的主要调节通道,这些调节通道蛋白的表达直接影响到机体内电解质的平衡。本文综述了与鱼类Na+和Cl-转运相关的主要载体蛋白的功能、影响其活力的因素及其调控机制等。

本文引用格式

吉中力 , 张春晓 , 麦康森 . 鱼类钠离子和氯离子转运载体的功能及调控机制研究进展[J]. 动物营养学报, 2016 , 28(2) : 369 -378 . DOI: 10.3969/j.issn.1006-267x.2016.02.009

Abstract

Not only Na+ and Cl- can participate the regulation of osmotic equilibrium and the balance of resting potential of cell membranes in fish, but the homeostasis of electrolyte in body. In the membrane of epithelial cells among gill, gastrointestinal tract and renal tubule of teleosts, there are Na+/K+-ATPase, Na+-K+-2Cl- cotransporter, Na+/H+ exchanger, cystic fibrosis transmembrane conductance regulator and the other transport proteins, those are the main control channels involving in the metabolism of Na+ and Cl-. The expression of proteins of these control channels can influence the electrolyte balance directly. This article summarized the function, factors influencing activity changes and regulatory mechanism of the main transport proteins which correlated with the transportation of Na+ and Cl-.

参考文献

[1] 庄青青.盐度胁迫下尼罗罗非鱼鳃离子细胞和Na+-K+-ATPase a1的渗透调节[D].硕士学位论文.上海:上海海洋大学,2013.
[2] KARNAKY K J,ERNST S A,PHILPOTT C W.Teleost chloride cell.Ⅰ.Response of pupfish Cyprinodon variegatus gill Na,K-ATPase and chloride cell fine structure to various high salinity environments[J].The Journal of Cell Biology,1976,70(1):144-156.  
[3] MACHADO M R.Uso de brânquias de peixes como indicadores de qualidade das águas[J].Unopar Científica Ciências Biológicas e da Saúde,1999,1(1):63-76.
[4] 李加儿,刘匆,段彪.提高鱼类渗透压调节能力研究进展[J].水产养殖,2002(3):30-32.
[5] TANG C H,LAI D Y,LEE T H.Effects of salinity acclimation on Na+/K+-ATPase responses and FXYD11 expression in the gills and kidneys of the Japanese eel (Anguilla japonica)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2012,163(3/4):302-310.
[6] BUCKING C,WOOD C M.Water dynamics in the digestive tract of the freshwater rainbow trout during the processing of a single meal[J].Journal of Experimental Biology,2006,209(10):1883-1893.  
[7] BUCKING C,WOOD C M.Gastrointestinal processing of Na+,Cl-,and K+ during digestion:implications for homeostatic balance in freshwater rainbow trout[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2006,291(6):1764-1772.  
[8] STURROCK A M,HUNTER E,MILTON J A,et al.Analysis methods and reference concentrations of 12 minor and trace elements in fish blood plasma[J].Journal of Trace Elements in Medicine and Biology,2013,27(4):273-285.  
[9] NORDLIE F G.Plasma osmotic,Na+ and Cl- regulation under euryhaline conditions in Cyprinodon variegatus lacépède[J].Comparative Biochemistry and Physiology Part A:Physiology,1987,86(1):57-61.  
[10] EVANS D H.Teleost fish osmoregulation:what have we learned since August Krogh,Homer Smith,and Ancel Keys[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2008,295(2):R704-R713.
[11] BUCKING C,WOOD C M,GROSELL M.Uptake,handling and excretion of Na+ and Cl- from the diet in vivo in freshwater- and seawater-acclimated killifish,Fundulus heteroclitus,an agastric teleost[J].Indian Journal of Experimental Biology,2013,216(20):3925-3936.  
[12] SCOTT G R,SCHULTE P M,WOOD C M.Plasticity of osmoregulatory function in the killifish intestine:drinking rates,salt and water transport,and gene expression after freshwater transfer[J].Journal of Experimental Biology,2006,209(20):4040-4050.  
[13] WOOD C M,BUCKING C,GROSELL M.Acid-base responses to feeding and intestinal Cl- uptake in freshwater-and seawater-acclimated killifish,Fundulus heteroclitus,an agastric euryhaline teleost[J].Blades on Ice,2010,213(Pt 15):2681-2692.
[14] EPSTEIN F H,MANITIUS A,WEINSTEIN E,et al.Sodium- and potassium-activated adenosine triphosphatase in kidneys of Fundulus heteroclitus adapted to fresh and salt water[J].Yale Journal of Biology & Medicine,1969,41(5):388-393.
[15] HWANG P P,LEE T H.New insights into fish ion regulation and mitochondrion-rich cells[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2007,148(3):479-497.  
[16] CUTLER C P,CRAMB G.Molecular physiology of osmoregulation in eels and other teleosts:the role of transporter isoforms and gene duplication[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2001,130(3):551-564.  
[17] MARSHALL W S.Na+,Cl-,Ca2+ and Zn2+ transport by fish gills:retrospective review and prospective synthesis[J].Journal of Experimental Zoology,2002,293(3):264-283.  
[18] EVANS D H,PIERMARINI P M,CHOE K P.The multifunctional fish gill:dominant site of gas exchange,osmoregulation,acid-base regulation,and excretion of nitrogenous waste[J].Physiological Reviews,2005,85(1):97-177.  
[19] LIN Y M,CHEN C N,LEE T H.The expression of gill Na,K-ATPase in milkfish,Chanos chanos,acclimated to seawater,brackish water and fresh water[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2003,135(3):489-497.  
[20] TANG C H,LEE T H.The effect of environmental salinity on the protein expression of Na+/K+-ATPase,Na+/K+/2Cl- cotransporter,cystic fibrosis transmembrane conductance regulator,anion exchanger 1,and chloride channel 3 in gills of a euryhaline teleost,Tetraodon nigroviridis[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2007,147(2):521-528.  
[21] LIN C H,LEE T H.Sodium or potassium ions activate different kinetics of gill Na,K-ATPase in three seawater- and freshwater-acclimated euryhaline teleosts[J].Journal of Experimental Zoology Part A:Comparative Experimental Biology,2005,203(1):57-65.
[22] HIROSE S,KANEKO T,NAITO N,et al.Molecular biology of major components of chloride cells[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2003,136(4):593-620.  
[23] HWANG P P,SUN C M,WU S M.Changes of plasma osmolality,chloride concentration and gill Na+-K+-ATPase activity in tilapia Oreochromis mossambicus during seawater acclimation[J].Marine Biology,1989,100(3):295-299.  
[24] LIN Y M,CHEN C N,YOSHINAGA T,et al.Short-term effects of hyposmotic shock on Na+/K+-ATPase expression in gills of the euryhaline milkfish,Chanos chanos[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2006,143(3):406-415.  
[25] KANG C K,TSAI S C,LEE T H,et al.Differential expression of branchial Na+/K+-ATPase of two medaka species,Oryzias latipes and Oryzias dancena,with different salinity tolerances acclimated to fresh water,brackish water and seawater[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2008,151(4):566-575.  
[26] UCHIDA K,KANEKO T,TAGAWA M,et al.Localization of cortisol receptor in branchial chloride cells in chum salmon fry[J].General and Comparative Endocrinology,1998,109(2):175-185.  
[27] D'COTTA H,VALOTAIRE C,GAC F L,et al.Synthesis of gill Na+-K+-ATPase in Atlantic salmon smolts:differences in α-mRNA and α-protein levels[J].American Journal of Physiology:Regulatory, Integrative and Comparative Physiology,2000,278(1):R101-R110.
[28] TIPSMARK C K,MADSEN S S,SEIDELIN M,et al.Dynamics of Na+,K+,2Cl- cotransporter and Na+,K+-ATPase expression in the branchial epithelium of brown trout (Salmo trutta) and atlantic salmon (Salmo salar)[J].Journal of Experimental Zoology,2002,293(2):106-118.  
[29] SCOTT G R,RICHARDS J G,FORBUSH B,et al.Changes in gene expression in gills of the euryhaline killifish (Fundulus heteroclitus) after abrupt salinity transfer[J].American Journal of Physiology:Cell Physiology,2004,287(2):C300-C309.
[30] TIPSMARK C K,MADSEN S S,BORSKI R J.Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis)[J].Journal of Experimental Zoology Part A:Comparative Experimental Biology,2004,301(12):979-991.
[31] RICHARDS J G,SEMPLE J W,BYSTRIANSKY J S,et al.Na+/K+-ATPase ɑ-isoform switching in gills of rainbow trout (Oncorhynchus mykiss) during salinity transfer[J].Journal of Experimental Biology,2003,206(24):4475-4486.  
[32] 冯平,王峰,范光丽.盐度对青鳉鱼肠内Na+-K+-ATPase基因表达的影响[J].西北农业学报,2006,15(6):24-27.
[33] SEALE A P,STAGG J J,YAMAGUCHI Y,et al.Effects of salinity and prolactin on gene transcript levels of ion transporters,ion pumps and prolactin receptors in Mozambique tilapia intestine[J].General and Comparative Endocrinology,2014,206:146-154.
[34] 吴庆元,蒋玫,李磊,等.低盐度胁迫对鲻鱼(Mugil cephalus)幼鱼鳃丝、肌肉、肠Na+-K+-ATP酶活性和MDA含量的影响[J].生态与农村环境学报,2014,30(4):481-487.
[35] GROSELL M.Intestinal anion exchange in marine fish osmoregulation[J].Journal of Experimental Biology,2006,209(15):2813-2827.  
[36] 张春晓,周磊,叶继丹,等.急性盐度胁迫对摄食不同镁水平饲料鲈血清渗透压和离子水平以及鳃丝ATP酶活力的影响[J].水产学报,2012,36(9):1425-1434.
[37] MCCORMICK S D.Endocrine control of osmoregulation in teleost fish[J].American Zoologist,2001,41(4):781-794.
[38] GROSELL M,GILMOUR K M,PERRY S F.Intestinal carbonic anhydrase,bicarbonate,and proton carriers play a role in the acclimation of rainbow trout to seawater[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2007,293(5):R2099-R2111.
[39] RUSSELL J M.Sodium-potassium-chloride cotransport[J].Physiological Reviews,2000,80(1):211-276.
[40] HEBERT S C,MOUNT D B,GAMBA G.Molecular physiology of cation-coupled Cl- cotransport:the SLC12 family[J].Pflügers Archiv,2004,447(5):580-593.
[41] GAMBA G.Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters[J].Physiological Reviews,2005,85(2):423-493.  
[42] 廖雅丽,张晨捷,高权新,等.鱼类离子细胞及离子通道的研究进展[J].海洋渔业,2015,37(1):77-86.
[43] CUTLER C P,CRAMB G.Two isoforms of the Na+/K+/2Cl- cotransporter are expressed in the European eel (Anguilla anguilla)[J].Biochimica et Biophysica Acta:Biomembranes,2002,1566(1/2):92-103.
[44] HIROI J,YASUMASU S,MCCORMICK S D,et al.Evidence for an apical Na-Cl cotransporter involved in ion uptake in a teleost fish[J].Journal of Experimental Biology,2008,211(16):2584-2599.  
[45] KANG C K,TSAI H J,LIU C C,et al.Salinity-dependent expression of a Na+,K+,2Cl- cotransporter in gills of the brackish medaka Oryzias dancena:a molecular correlate for hyposmoregulatory endurance[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2010,157(1):7-18.  
[46] CUTLER C P,CRAMB G.Differential expression of absorptive cation-chloride-cotransporters in the intestinal and renal tissues of the European eel (Anguilla anguilla)[J].Comparative Biochemistry and Physiology Part B:Biochemistry & Molecular Biology,2008,149(1):63-73.  
[47] TRESGUERRES M,LEVIN L R,BUCK J,et al.Modulation of NaCl absorption by HCO-3 in the marine teleost intestine is mediated by soluble adenylyl cyclase[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2010,299(1):R62-R71.
[48] SCOTT G R,KEIR K R,SCHULTE P M.Effects of spironolactone and RU486 on gene expression and cell proliferation after freshwater transfer in the euryhaline killifish[J].Journal of Comparative Physiology B,2005,175(7):499-510.  
[49] 范武江,李思发.萨罗罗非鱼NKCC1α基因cDNA克隆及mRNA组织表达差异[J].动物学研究,2010,31(6):601-609.
[50] HIROI J,MCCORMICK S D.Variation in salinity tolerance,gill Na+/K+-ATPase,Na+/K+/2Cl- cotransporter and mitochondria-rich cell distribution in three salmonids Salvelinus namaycush,Salvelinus fontinalis and Salmo salar[J].Journal of Experimental Biology,2007,210(6):1015-1024.  
[51] LORIN-NEBEL C,BOULO V,BODINIER C,et al.The Na+/K+/2Cl- cotransporter in the sea bass Dicentrarchus labrax during ontogeny:involvement in osmoregulation[J].Journal of Experimental Biology,2006,209(24):4908-4922.  
[52] 邵占涛.鲈鱼头肾cDNA文库的构建与免疫相关基因的筛选及中肾NCC基因的研究[D].博士学位论文.济南:山东师范大学,2009.
[53] BREVES J P,SERIZIER S B,GOFFIN V,et al.Prolactin regulates transcription of the ion uptake Na+/Cl- cotransporter (NCC) gene in zebrafish gill[J].Molecular and Cellular Endocrinology,2013,369(1/2):98-106.
[54] HSU H H,LIN L Y,TSENG Y C,et al.A new model for fish ion regulation:identification of ionocytes in freshwater- and seawater-acclimated medaka (Oryzias latipes)[J].Cell and Tissue Research,2014,357(1):225-243.  
[55] INOKUCHI M,HIROI J,WATANABE S,et al.Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia[J].Journal of Experimental Biology,2009,212(7):1003-1010.  
[56] HAYASHI H,SZÁSZI K,GRINSTEIN S.Multiple modes of regulation of Na+/H+ exchangers[J].Annals of the New York Academy of Sciences,2002,976:248-258.
[57] ORLOWSKI J,GRINSTEIN S.Na+/H+ exchangers of mammalian cells[J].The Journal of Biological Chemistry,1997,272(36):22373-22376.  
[58] PARKS S K,TRESGUERRES M,GOSS G G.Theoretical considerations underlying Na+ uptake mechanisms in freshwater fishes[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2008,148(4):411-418.  
[59] KROGH A.The active absorption of ions in some freshwater animals[J].Journal of Comparative Physiology,1938,25(3):335-350.
[60] YAN J J,CHOU M Y,KANEKO T,et al.Gene expression of Na+/H+ exchanger in zebrafish H+ -ATPase-rich cells during acclimation to low-Na+ and acidic environments[J].The American Journal of Physiology:Cell Physiology,2007,293(6):C1814-C1823.
[61] ESAKI M,HOSHIJIMA K,KOBAYASHI S,et al.Visualization in zebrafish larvae of Na+ uptake in mitochondria-rich cells whose differentiation is dependent on foxi3a[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2007,292(1):R470-R480.
[62] CHOE K P,KATO A,HIROSE S,et al.NHE3 in an ancestral vertebrate:primary sequence,distribution,localization,and function in gills[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2005,289(5):R1520-R1534.
[63] WATANABE S,NIIDA M,MARUYAMA T,et al.Na+/H+ exchanger isoform 3 expressed in apical membrane of gill mitochondrion-rich cells in Mozambique tilapia Oreochromis mossambicus[J].Fisheries Science,2008,74(4):813-821.  
[64] RIMOLDI S,TEROVA G,BRAMBILLA F,et al.Molecular characterization and expression analysis of Na+/H+ exchanger (NHE)-1 and c-Fos genes in sea bass (Dicentrarchus labrax,L) exposed to acute and chronic hypercapnia[J].Journal of Experimental Marine Biology and Ecology,2009,375(1/2):32-40.
[65] IVANIS G,BRAUN M,PERRY S F.Renal expression and localization of SLC9A3 sodium/hydrogen exchanger and its possible role in acid-base regulation in freshwater rainbow trout (Oncorhynchus mykiss)[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2008,295(3):R971-R978.
[66] HIRATA T,KANEKO T,ONO T,et al.Mechanism of acid adaptation of a fish living in a pH 3.5 lake[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2003,53(5):R1199-R1212.
[67] SCOTT G R,CLAIBORNE J B,EDWARDS S L,et al.Gene expression after freshwater transfer in gills and opercular epithelia of killifish:insight into divergent mechanisms of ion transport[J].Journal of Experimental Biology,2005,208(14):2719-2729.  
[68] WALL B,MORRISON-SHETLAR A I,CLAIBORNE J B.Effects of environment salinity and hypercapnia on NHE2-like and NHE3-like protein expression in the gill of the mumichog (Fundulus heteroclitus)[J].Bulletin Mount Desert Island Biological Laboratory,2001,40:58-59.
[69] EDWARDS S L,CLAIBORNE J B,MORRISON-SHETLAR A I,et al.Expression of Na+/H+ exchanger mRNA in the gills of Atlantic hagfish (Myxine glutinosa) in response to metabolic acidosis[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2001,130(1):81-91.  
[70] MARSHALL W S,WATTERS K D,HOVDESTAD L R,et al.CFTR Cl- channel functional regulation by phosphorylation of focal adhesion kinase at tyrosine 407 in osmosensitive ion transporting mitochondria rich cells of euryhaline killifish[J].Journal of Experimental Biology,2009,212(15):2365-2377.  
[71] SINGER T D,TUCKER S J,MARSHALL W S,et al.A divergent CFTR homologue:highly regulated salt transport in the euryhaline teleost F.heteroclitus[J].American Journal of Physiology:Cell Physiology Published,1998,274(3 Pt 1):C715-C723.
[72] HIROI J,MCCORMICK S D,OHTANI-KANEKO R,et al.Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos,by means of triple immunofluorescence staining for Na+/K+-ATPase,Na+/K+/2Cl- cotransporter and CFTR anion channel[J].Journal of Experimental Biology,2005,208(11):2023-2036.  
[73] SINGER T D,CLEMENTS K M,SEMPLE J W,et al.Seawater tolerance and gene expression in two strains of Atlantic salmon smolts[J].Canadian Journal of Fisheries and Aquatic Sciences,2002,59(1):125-135.  
文章导航

/