本试验旨在研究1~21日龄爱拔益加(AA)×罗曼肉杂鸡饲粮代谢能、粗蛋白质、蛋氨酸和赖氨酸的适宜水平。采用L₉(3⁴)正交试验设计,饲粮代谢能水平分别为11.70、12.12、12.54 MJ/kg;粗蛋白质水平分别为19.00%、20.00%、21.00%;蛋氨酸水平分别为0.45%、0.50%、0.55%;赖氨酸水平分别为1.00%、1.10%、1.20%。选取1日龄AA×罗曼肉杂鸡864只,随机分为9组,每组6个重复,每个重复16只,试验期21 d。通过饲养试验、屠宰试验等方法检测不同营养水平饲粮的饲喂效果。结果表明:1)饲粮粗蛋白质、蛋氨酸和赖氨酸水平对试验鸡平均日增重有显著影响(P<0.05),21.00%粗蛋白质水平组显著高于20.00%粗蛋白质水平组(P<0.05),0.50%蛋氨酸水平组显著高于0.55%蛋氨酸水平组(P<0.05),1.20%赖氨酸水平组显著高于1.10%赖氨酸水平组(P<0.05)。2)饲粮代谢能水平对21日龄试验鸡活体总能含量有显著影响(P<0.05),12.12 MJ/kg代谢能水平组显著高于12.54 MJ/kg代谢能水平组(P<0.05);饲粮蛋氨酸水平对试验鸡活体粗脂肪含量有显著影响(P<0.05),0.50%和0.55%蛋氨酸水平组显著高于0.45%蛋氨酸水平组(P<0.05)。3)饲粮代谢能和粗蛋白质水平对21日龄试验鸡活体能量沉积率有显著影响(P<0.05),11.70和12.12 MJ/kg代谢能水平组显著高于12.54 MJ/kg代谢能水平组(P<0.05),21.00%粗蛋白质水平组显著高于20.00%和19.00%粗蛋白质水平组(P<0.05);1.10%赖氨酸水平组的试验鸡活体赖氨酸沉积率显著高于1.20%赖氨酸水平组(P<0.05)。4)饲粮赖氨酸水平对试验鸡血清葡萄糖、尿酸、尿素氮水平有显著影响(P<0.05),1.00%赖氨酸水平组血清葡萄糖水平最高,显著高于1.20%赖氨酸水平组(P<0.05),1.10%赖氨酸水平组血清尿酸水平最高,显著高于1.20%赖氨酸水平组(P<0.05),1.20%赖氨酸水平组血清尿素氮水平最高,显著高于1.10%赖氨酸水平组(P<0.05)。5)由回归分析得出1~21日龄AA×罗曼肉杂鸡饲粮代谢能、粗蛋白质、蛋氨酸和赖氨酸适宜水平的计算公式分别为ME=45.33W^0.75+183.84△W,CP=19.77W^0.75+626.47△W,Met=1.44W^0.75+10.31△W,Lys=3.01W^0.75+21.28△W(ME为代谢能,W^0.75为代谢体重,△W为平均日增重,CP为粗蛋白质,Met为蛋氨酸,Lys为赖氨酸),根据公式计算得出饲粮代谢能、粗蛋白质、蛋氨酸和赖氨酸的适宜水平分别为11.90 MJ/kg、21.08%、0.51%和1.05%。

The optimal levels of metabolic energy (ME), crude protein (CP), methionine (Met) and lysine (Lys) of Arbor Acres(AA)×Roman hybrid broilers aged from 1 to 21 days were studied in this experiment. The L₉(3⁴)orthogonal experiment design was used, ME levels were 11.70, 12.12 and 12.54 MJ/kg, respectively, CP levels were 19.00%, 20.00% and 21.00%, respectively, Met levels were 0.45%, 0.50% and 0.55%, respectively, Lys levels were 1.0%, 1.1% and 1.20%, respectively. The broilers were randomly assigned into 9 groups with 6 replicates per group and 16 broilers per replicate. The experiment lasted for 21 days. The feeding effects of diets with different nutrient levels were determined by feeding experiment, comparative slaughter experiment and other methods. The results showed as follows:1) the levels of CP, Met and Lys in diets had significant effect on the average daily gain (P<0.05), which in 21.00% CP level group was significantly higher than that in 20.00% CP level group (P<0.05), in 0.50% Met level group was significantly higher than that in 0.55% Met level group (P<0.05), and in 1.20% Lys level group was significantly higher than that in 1.10% Lys level group (P<0.05). 2) The ME level in diets had significant effect on the content of body total energy of 21-day-old broilers (P<0.05), which in 12.12 MJ/kg ME level group was significantly higher than that in 12.54 MJ/kg ME level group (P<0.05). The Met level in diets had significant effect on the content of body ether extract (P<0.05), which in 0.50% and 0.55% Met level groups was significantly higher than that in 0.45% Met level group (P<0.05). 3) The levels of ME and CP in diets had significant effect on the body energy deposition rate of 21-day-old broilers, which in 11.70 and 12.12 MJ/kg ME level group was significantly higher than that in 12.54 MJ/kg ME level group (P<0.05), and in 21.00% CP level group was significantly higher than that in 20.00% and 19.00% CP level group (P<0.05). The body Lys deposition rate of 21-day-old broilers in 1.10% Lys level group was significantly higher than that in 1.20% Lys level group (P<0.05). 4) The Lys level in diets had significant effects on the levels of glucose, uric acid and urea nitrogen in serum of 21-day-old broilers (P<0.05), the glucose level in serum in 1.00% Lys level group was the highest, which was significantly higher than that in 1.20% Lys level group (P<0.05), the uric acid level in serum in 1.10% Lys level group was the highest, which was significantly higher than that in 1.20% Lys level group(P<0.05), and the urea nitrogen level in 1.20% Lys level group was the highest, which was significantly higher than that in 1.10% Lys level group(P<0.05). 5) The regression analysis showed that the optimal level formulas of ME, CP, Met and Lys were ME=45.33W^0.75+183.84△W, CP=19.77W^0.75+626.47△W, Met=1.44W^0.75+10.31△W, Lys=3.01W^0.75+21.28△W (ME was metabolic energy, W^0.75 was metabolic weight, △W was average daily gain, CP was crude protein,Met was methionine and Lys was lysine), respectively. According to the above formulas, the calculated optimal levels of ME, CP, Met and Lys of AA×Roman hybrid broilers aged from 1 to 21 days are 11.90 MJ/kg, 21.08%, 0.51% and 1.05%, respectively.