动物营养学报 >

2016 , Vol. 28 >Issue 2: 418 - 425

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.015

反刍动物营养 Ruminant Nutrition

添加过瘤胃蛋氨酸、赖氨酸对荷斯坦奶公牛生长性能和胴体化学成分的影响

  • 韩云胜 ,
  • 曲永利 ,
  • 袁雪 ,
  • 王志博 ,
  • 殷溪瀚 ,
  • 李伟 ,
  • 潘琪琪 ,
  • 王冠 ,
  • 高岩
展开
  • 黑龙江八一农垦大学动物科技学院, 大庆 163319

收稿日期: 2015-09-01

  网络出版日期: 2016-02-19

基金资助

国家科技部支撑项目"东北农区奶牛规模化健康养殖生产技术集成和产业化示范"(2012BAD12B05-01);黑龙江省农垦总局项目"黑龙江垦区规模化奶牛场增产关键技术研究与示范"(HNK125B-11-02)

收起

Effects of Rumen-Protected Methionine and Lysine Supplementations on Growth Performance and Carcass Chemical Composition of Holstein Bulls

  • HAN Yunsheng ,
  • QU Yongli ,
  • YUAN Xue ,
  • WANG Zhibo ,
  • YIN Xihan ,
  • LI Wei ,
  • PAN Qiqi ,
  • WANG Guan ,
  • GAO Yan
Expand
  • College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2015-09-01

  Online published: 2016-02-19

Fold

摘要

本文旨在研究在基础饲粮中添加过瘤胃蛋氨酸(RPMet)、过瘤胃赖氨酸(RPLys)对荷斯坦奶公牛生长性能和胴体化学成分的影响,探讨用RPMet和RPLys替代部分饲粮粗蛋白质的可行性。试验采用完全随机设计,选择12月龄左右,体重、体尺相近的健康奶公牛25头,随机分为5组,每组5头。对照组饲喂基础饲粮,4个试验组M15、L30、M15+L30、LCP组分别在基础饲粮中添加RPMet 15 g/d、RPLys 30 g/d、RPMet 15 g/d+RPLys 30 g/d、RPMet 15 g/d+RPLys 30 g/d(同时降低饲粮粗蛋白质2.20%)。预试期10 d,正试期150 d。结果表明,与对照组相比:1)基础饲粮中添加RPMet和RPLys对奶公牛干物质采食量无显著影响(P>0.05);L30、M15+L30、LCP组末重和平均日增重显著增加(P<0.05),且均以M15+L30组最高,但M15+L30组与LCP组无显著差异(P>0.05);M15+L30和LCP组料重比显著降低(P<0.05),以M15+L30组最低,这2组间差异不显著(P>0.05)。2)M15+L30和LCP组奶公牛胸围和胴体中粗蛋白质含量显著提高(P<0.05),这2组间差异不显著(P>0.05)。3)M15+L30和LCP组奶公牛胴体中必需氨基酸苏氨酸、亮氨酸、精氨酸的含量显著提高(P<0.05),这2组间差异不显著(P>0.05),M15+L30组异亮氨酸含量显著提高(P<0.05);M15+L30和LCP组奶公牛胴体中非必需氨基酸中天冬氨酸、丝氨酸、谷氨酸、甘氨酸、丙氨酸、半胱氨酸、脯氨酸以及总氨基酸含量显著提高(P<0.05),L30和M15+L30组酪氨酸含量显著提高(P<0.05),这2组间均无显著差异(P>0.05)。由此可见,饲粮中添加RPMet和RPLys在一定程度上提高了奶公牛的生长性能,增加了胴体中蛋白质的合成和氨基酸沉积,且添加RPMet 15 g/d+RPLys 30 g/d能够在一定程度上替代部分饲粮粗蛋白质(2.20%)。

关键词： 过瘤胃蛋氨酸; 过瘤胃赖氨酸; 荷斯坦奶公牛; 生长性能; 胴体化学成分

本文引用格式

韩云胜 , 曲永利 , 袁雪 , 王志博 , 殷溪瀚 , 李伟 , 潘琪琪 , 王冠 , 高岩 . 添加过瘤胃蛋氨酸、赖氨酸对荷斯坦奶公牛生长性能和胴体化学成分的影响[J]. 动物营养学报, 2016 , 28(2) : 418 -425 . DOI: 10.3969/j.issn.1006-267x.2016.02.015

Abstract

This experiment was conducted to investigate the effects of rumen-protected methionine (RPMet) and lysine (RPLys) supplementations on growth performance and carcass chemical composition of Holstein bulls, and to discuss the feasibility that RPMet and RPLys instead of part protein of diet. Twenty five Holstein bulls aged about 12 months with similar body weight and body measurements were selected and divided into 5 groups with randomized experiment design, and each group included 5 Holstein bulls. The control group was fed a basal diet, the experimental groups were fed the basal diet added with RPMet 15 g/d (M15 group), RPLys 30 g/d (L30 group), RPMet 15 g/d+RPLys 30 g/d (M15+L30 group) and RPMet 15 g/d+RPLys 30 g/d (meanwhile dietary crude protein content was lowered by 2.20%) (LCP group), respectively. Adaptation and experimental periods lasted for 10 and 150 d, respectively. The results showed that compared with control group:1) dry matter intake was not affected by RPMet and RPLys supplementations (P>0.05); L30, M15+L30, LCP groups significantly increased final weight and average daily gain (P<0.05), and the values reached the highest in M15+L30 group, but they were not significantly different from those in LCP group (P>0.05); M15+L30 and LCP groups significantly decreased feed to gain ratio (P<0.05), and M15+L30 group had the minimum value, but the difference between M15+L30 and LCP groups was not significant (P>0.05). 2) Chest girth and carcass crude protein content in M15+L30 and LCP groups were significantly increased (P<0.05), while there was no significant difference between the two groups (P>0.05). 3) M15+L30 and LCP groups significantly increased carcass essential amino acid contents including threonine, leucine and arginine (P<0.05), and the differences between the two groups were not significant (P>0.05); M15+L30 group significantly increased isoleucine content (P<0.05); M15+L30 and LCP groups had significantly higher non-essential amino acid contents including aspartate, serine, glutamic acid, glycine, alanine, cystine and proline and total amino acid content (P<0.05); L30 and M15+L30 groups had significantly higher tyrosine content (P<0.05), but there was no significantly difference between the two groups (P>0.05). It is concluded that dietary supplementations of RPMet and RPLys can improve growth performance and increase carcass protein and amino acid contents of Holstein bulls, and the supplementation of RPMet 15 g/d+RPLys 30 g/d can instead part of dietary crude protein (2.20%).

Key words： RPMet; RPLys; Holstein bulls; growth performance; carcass chemical composition

参考文献

[1] 贾文彬,李建国,赵世芳.反刍动物过瘤胃氨基酸的研究进展[J].饲料博览,2005(12):10-12.
[2] 冯薇,王加启,卜登攀.奶牛过瘤胃蛋氨酸和过瘤胃赖氨酸营养研究进展[J].中国奶牛,2009(12):17-21.
[3] NOFTSGER S,ST-PIERRE N R.Supplementation of methionine and selection of highly digestible rumen undegradable protein to improve nitrogen efficiency for milk production[J].Journal of Dairy Science,2003,86(3):958-969.  
[4] 丁洪涛,吕荣创.肥育牛日粮中添加瘤胃保护氨基酸的研究[J].饲料研究,2011(1):54-55.
[5] 倪丽丽,闫素梅,赵鹏,等.粗蛋白与过瘤胃蛋白对奶牛氮排泄的影响[J].饲料研究,2011(7):60-62.
[6] 郭亮,王治华,蔡治华,等.营养水平对荷斯坦肥育牛胴体品质及肉品质量的影响[J].中国兽医学报,2008,28(10):1225-1228,1238.
[7] 冯仰廉,王加启,杨红建,等.肉牛饲养标准[M].北京:中国农业出版社,2004.
[8] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003:24-68.
[9] AOAC.Official methods of analysis of the Association of Official Analytical Chemists[S].15th ed.Arlington:Association of Analytical Chemists,1990.
[10] VEIRA D M,SEOANE J R,PROULX J G.Utilization of grass silage by growing cattle:effect of a supplement containing ruminally protected amino acids[J].Journal of Animal Science,1991,69(12):4703-4711.
[11] GREENWOOD R H,TITGEMEYER E C.Limiting amino acids for growing Holstein steers limit-fed soybean hull-based diets[J].Journal of Animal Science,2000,78(7):1997-2004.
[12] ABE M,IRIKI T,KANESHIGE K,et al.Adverse effects of excess lysine in calves[J].Journal of Animal Science,2001,79(5):1344-1345.
[13] 云强,刁其玉,屠焰,等.日粮中赖氨酸和蛋氨酸比对断奶犊牛生长性能和消化代谢的影响[J].中国农业科学,2011,44(1):133-142.
[14] ARCHIBEQUE S L,BURNS J C,HUNTINGTON G B.Nitrogen metabolism of beef steers fed endophyte-free tall fescue hay:effects of ruminally protected methionine supplementation[J].Journal of Animal Science,2002,80(5):1344-1351.
[15] 蔺宏凯,张杨,周振勇,等.新疆褐牛体尺性状指标与体重的主成分分析[J].中国畜牧兽医,2010,37(8):130-133.
[16] OWENS F N,DUBESKI P,HANSON C F.Factors that alter the growth and development of ruminants[J].Journal of Animal Science,1993,71(11):3138-3150.
[17] 薛丰,郭晓旭,郭望山,等.日粮添加过瘤胃赖氨酸对利木赞杂交肉牛胴体性状和肉质的影响[J].中国农业大学学报,2010,15(4):82-86.
[18] HUSSEIN H S,BERGER L L.Feedlot performance and carcass characteristics of Holstein steers as affected by source of dietary protein and level of ruminally protected lysine and methionine[J].Journal of AnimalScience,1995,73(12):3503-3509.
[19] MITCHELL H H,BLOCK R J.Some relationships between the amino acid contents of proteins and their nutritive values for the rat[J].Journal of Biological Chemistry,1964,163:599-620.
[20] 黄红英,贺建华,范志勇,等.母猪日粮中支链氨基酸水平对仔猪血液生化指标和部分免疫指标的影响[J].饲料工业,2007,28(21):24-26.
[21] ERASMUS L J,BOTHA P M,MEISSNER H H.Effect of protein source on ruminal fermentation and passage of amino acids to the small intestine of lactating cows[J].Journal of Dairy Science,1994,77(12):3655-3665.  
[22] 甄玉国.内蒙古白绒山羊氨基酸利用和蛋白质周转规律的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2002.
[23] 王洪荣,徐爱秋,王梦芝,等.氨基酸对体外培养瘤胃微生物生长及发酵的影响[J].畜牧兽医学报,2010,41(9):1109-1116.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2