动物营养学报 >

2016 , Vol. 28 >Issue 2: 426 - 435

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.016

反刍动物营养 Ruminant Nutrition

金黄色葡萄球菌诱导型乳腺炎对中国荷斯坦奶牛乳中脂肪酸组成的影响

  • 李蕊 ,
  • 张成龙 ,
  • 耿晓晗 ,
  • 李锐 ,
  • 蒲俊华 ,
  • 毛永江 ,
  • 冀德君 ,
  • 杨章平 ,
  • 吴苏红 ,
  • 徐向前 ,
  • 陆春兵 ,
  • 任湘莲
展开
  • 1. 扬州大学动物科学与技术学院, 扬州 225009;
    2. 江苏省江阴市动物疫病预防控制中心, 江阴 214400

收稿日期: 2015-07-26

  网络出版日期: 2016-02-19

基金资助

国家自然科学基金(31272407,31472067);江苏省农业三新工程项目(SXGC[2015]328)

收起

Effects of Staphylococcus aureus-Induced Mastitis on Milk Fatty Acid Composition of Chinese Holstein Cows

  • LI Rui ,
  • ZHANG Chenglong ,
  • GENG Xiaohan ,
  • LI Rui ,
  • PU Junhua ,
  • MAO Yongjiang ,
  • JI Dejun ,
  • YANG Zhangping ,
  • WU Suhong ,
  • XU Xiangqian ,
  • LU Chunbing ,
  • REN Xianglian
Expand
  • 1. Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China;
    2. Animal Disease Prevention and Control Center, Jiangsu Province, Jiangyin 214400, China

Received date: 2015-07-26

  Online published: 2016-02-19

Fold

摘要

本文旨在研究金黄色葡萄球菌诱导型乳腺炎对中国荷斯坦奶牛乳中脂肪酸组成及其绝对含量的影响。根据奶牛乳房结构,试验组利用通乳针将金黄色葡萄球菌细菌悬液经乳头管注射入奶牛乳房内,建立诱导型金黄色葡萄球菌型乳腺炎模型,对照组灌注磷酸盐缓冲液,采用气相色谱法测定乳中的脂肪酸绝对含量。结果表明:乳中共检出34种脂肪酸,金黄色葡萄球菌诱导对感染乳区脂肪酸合成产生直接影响。按不饱和度和碳链长度划分的6大类脂肪酸中,试验组在感染后11 h与感染前24 h相比,短链脂肪酸、中链脂肪酸和饱和脂肪酸的绝对含量均显著降低(P<0.05);对照组有相同变化趋势,但除短链脂肪酸外均未达到显著水平(P>0.05)。感染后23 h试验组6大类脂肪酸的绝对含量均低于对照组。结果提示:金黄色葡萄球菌诱导型乳腺炎影响了奶牛脂肪酸的合成过程,降低了脂肪酸各组分的绝对含量。

关键词： 中国荷斯坦奶牛; 脂肪酸; 金黄色葡萄球菌; 乳腺炎

本文引用格式

李蕊 , 张成龙 , 耿晓晗 , 李锐 , 蒲俊华 , 毛永江 , 冀德君 , 杨章平 , 吴苏红 , 徐向前 , 陆春兵 , 任湘莲 . 金黄色葡萄球菌诱导型乳腺炎对中国荷斯坦奶牛乳中脂肪酸组成的影响[J]. 动物营养学报, 2016 , 28(2) : 426 -435 . DOI: 10.3969/j.issn.1006-267x.2016.02.016

Abstract

The aim of this study was to explore the effects of Staphylococcus aureus (S. aureus)-induced mastitis on milk fatty acid composition and their absolute content of Chinese Holstein cows. The bacterial suspension of S. aureus was administered into the udder to establish a S. aureus-induced mastitis model in experimental group, and PBS was administered in control group. Fatty acid absolute contents in milk were determined by gas chromatography. The results showed as follows:thirty four fatty acids were detected in milk, and fatty acid synthesis at breast area was directly affected by S. aureus-induction. The fatty acids were divided into six categories by degree of saturation and carbon chain length, and compared with 24 h before infection, the absolute contents of short chain fatty acids (sc-FA), medium chain fatty acid (mc-FA) and saturated fatty acid (SFA) at 11 h after infection in experimental group were significantly lower (P<0.05); control group had the same changing tendency, but there were no significant differences (P>0.05) except sc-FA. At 23 h after infection, the absolute content of all six categories of fatty acids in experimental group lower than those in control group. The results indicate that the dynamics of fatty acids had the same trend. These results demonstrated that S. aureus-induced mastitis can affect the synthesis of cow fatty acids and lowered the absolute content of each component.

Key words： Chinese Holstein cows; fatty acids; Staphylococcus aureus; mastitis

参考文献

[1] 王玲,李宏胜,王正兵,等.规模化奶牛养殖区奶牛乳房炎的调查及病因分析[J].中国乳业,2010(10):50-53.
[2] SINGLA M,SHARMA A K,PARMAR O S,et al.Effect of bedding material of different depths on milk yield and udder health of cross bred cows during the winter months[J].Veterinary Practitioner,2007,8(1):73-77.
[3] 尹荣兰,杨正涛,张艳静,等.金黄色葡萄球菌CIfA基因的克隆及在大肠杆菌中的表达[J].中国兽医学报,2009,29(2):150-152.
[4] CHANDLER R L.Experimental bacterial mastitis in the mouse[J].Journal of Medical Microbiology,1970,3(2):273-282.  
[5] BRAMLEY A J,FOSTER R.Effects of lysostaphin on Staphylococcus aureus infections of the mouse mammary gland[J].Research in Veterinary Science,1990,49(1):120-121.
[6] HU C M,GONG R,GUO A Z,et al.Protective effect of ligand-binding domain of fibronectin-binding protein on mastitis induced by Staphylococcus aureus in mice[J].Vaccine,2010,28(24):4038-4044.  
[7] GUO M Y,ZHANG N S,LI D P,et al.Baicalin plays an anti-inflammatory role through reducing nuclear factor-κB and p38 phosphorylation in S. aureus-induced mastitis[J].International Immunopharmacology,2013,16(2):125-130.  
[8] 谢菲,韩旭东,高新志,等.金黄色葡萄球菌诱发小鼠乳腺炎模型的建立[J].现代农业科技,2008(21):18-19.
[9] 杨天骄,孙英峰,沈国顺.小鼠模型在奶牛金黄色葡萄球菌乳房炎中的应用[J].中国畜牧兽医,2008,35(12):116-118.
[10] 张宝君,崔京春,张献,等.金黄色葡萄球菌乳腺感染模型的建立与评价[J].中国预防兽医学报,2009,31(5):365-369.
[11] 战永波,王纯洁,包金荣,等.蒙药对奶牛乳房炎中大肠杆菌和金黄色葡萄球菌的抑制作用[J].黑龙江畜牧兽医,2010(2):134-135.
[12] 杨峰,王旭荣,田永刚,等.牛源金黄色葡萄球菌感染小鼠后药物敏感性变化及其诱导的氧化应激[J].动物医学进展,2013,34(12):46-50.
[13] 袁峥嵘.金黄色葡萄球菌诱导奶牛实验性乳腺炎研究[D].博士学位论文.北京:中国农业科学院,2011.
[14] 刘利,高雪,高会江,等.DNA甲基化在奶牛金黄色葡萄球菌性乳房炎中的调控[J].畜牧兽医学报,2012,43(10):1554-1558.
[15] 崔新洁.金黄色葡萄球菌诱导牛乳腺上皮细胞凋亡及其信号通路的研究[D].硕士学位论文.呼和浩特:内蒙古大学,2013.
[16] 农业部食品质量监督检验测试中心.GB/T 21676-2008乳与乳制品脂肪酸的测定气相色谱法[S].北京:中国标准出版社,2008.
[17] 林秋萍,李瑾,冯书惠.气相色谱法快速测定牛奶中脂肪酸[J].食品科学,2005,26(8):346-348.
[18] 李云龙,杨章平,常玲玲,等.气相色谱法检测牛奶中脂肪酸组分方法的改进[J].中国牛业科学,2012,38(3):18-21.
[19] HALASA T,NIELEN M,HUIRNE R B M,et al.Stochastic bio-economic model of bovine intramammary infection[J].Livestock Science,2009,124(1/2/3):295-305.
[20] MARÉChAl C L,THIÉRY R,VAUTOR E,et al.Mastitis impact on technological properties of milk and quality of milk products-a review[J].Dairy Science & Technology,2011,91(3):247-282.  
[21] 杨德英,曹随忠,余树民,等.奶牛隐性乳房炎对牛奶品质的影响[J].湖北农业科学,2009,48(1):132-134.
[22] 常玲玲.中国荷斯坦牛乳中脂肪酸变化规律及其与脂肪酸合成相关基因的关联分析[D].硕士学位论文.扬州:扬州大学,2011.
[23] 臧丽,李英俊,张乃生,等.奶牛乳腺的防御机制与乳腺炎病理学[J].动物医学进展,2006,27(11):33-37.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2