地衣芽孢杆菌对肉羊甲烷排放及消化代谢的影响

肖怡; 陶大勇; 赵明明; 赵江波; 马涛; 屠焰; 刁其玉

doi:10.3969/j.issn.1006-267x.2016.02.025

动物营养学报 >

2016 , Vol. 28 >Issue 2: 515 - 523

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.025

饲料营养 Feed Science and Technology

地衣芽孢杆菌对肉羊甲烷排放及消化代谢的影响

肖怡 ,
陶大勇 ,
赵明明 ,
赵江波 ,
马涛 ,
屠焰 ,
刁其玉

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1. 塔里木大学动物科学学院, 阿拉尔 843300;
2. 中国农业科学院饲料研究所, 农业部饲料生物技术重点实验室, 北京 100081

收稿日期: 2015-08-14

网络出版日期: 2016-02-19

基金资助

国家自然基金"绵羊甲烷排放的粪便反射特征光谱研究"(41475126);国家科技支撑计划"农区肉羊健康养殖模式构建与示范"(2012BAD39B05-3)

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Effects of Baclicus lincheniformis on Methane Emission, Digestion and Metabolism of Mutton Sheep

XIAO Yi ,
TAO Dayong ,
ZHAO Mingming ,
ZHAO Jiangbo ,
MA Tao ,
TU Yan ,
DIAO Qiyu

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1. College of Animal Science, Tarim University, Alaer 843300, China;
2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, China

Received date: 2015-08-14

Online published: 2016-02-19

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摘要

本试验旨在研究饲粮中不同添加水平的地衣芽孢杆菌对肉羊甲烷排放及消化代谢的影响,为微生态制剂减少肉羊甲烷排放和提高饲料利用率提供依据。试验选取24只体重为(45.00±1.96) kg、体况良好的杜泊(♂)×小尾寒羊(♀)杂交F1代成年羯羊,采用单因素随机区组设计,将其分为4组,每组6只羊,每只羊为1个重复,对照组饲喂基础饲粮,3个试验组基础饲粮中地衣芽孢杆菌的添加水平分别为2.4×10⁸、2.4×10⁹和2.4×10¹⁰ CFU/(只·d),分别记作低、中、高剂量组。试验期20 d,其中预试期8 d,正试期12 d。采用Sable开路式循环测热系统测定甲烷排放量,采用全收粪尿法测定消化代谢指标。结果表明:1)与对照组相比,低、中剂量组显著降低了肉羊干物质采食量和代谢体重基础的甲烷排放量[L/(kg W^0.75·kg DMI)](P<0.05),降幅分别为5.18%和9.33%。2)与对照组相比,试验组均显著提高了肉羊干物质、有机物、中性洗涤纤维、酸性洗涤纤维(除低剂量组)、氮的表观消化率和沉积氮(P<0.05),并显著提高了饲粮消化能和代谢能(P<0.05)。本试验条件下,添加地衣芽孢杆菌可在一定程度上降低肉羊的甲烷排放量,提高营养物质消化率,进而提高饲料的能量利用率。

关键词： 微生态制剂; 地衣芽孢杆菌; 甲烷; 消化代谢; 肉羊

本文引用格式

肖怡 , 陶大勇 , 赵明明 , 赵江波 , 马涛 , 屠焰 , 刁其玉 . 地衣芽孢杆菌对肉羊甲烷排放及消化代谢的影响[J]. 动物营养学报, 2016 , 28(2) : 515 -523 . DOI: 10.3969/j.issn.1006-267x.2016.02.025

Abstract

This study was conducted to investigate the effects of dietary supplementation of different levels of Baclicus lincheniformis on methane emission, digestion and metabolism of mutton sheep, with an aim to provide reference of probiotics for reducing methane emission and improving energy efficiency. Twenty-four healthy Dorper (♂)×thin-tailed Han (♀) crossbred adult wethers (F1) with an average body weight of (45.00±1.96) kg were randomly divided into 4 groups with 6 sheep in each group and each wether as one replicate by an one-factor design, and fed one of the following 4 diets:a basal diet (control group) and the basal diet supplemented with 2.4×10⁸ (low dose group), 2.4×10⁹ (medium dose group) and 2.4×10¹⁰ CFU/d (high dose group) Baclicus lincheniformis per lamb. The experiment lasted for 20 days, including an 8-day adaptation period, and for the following 12 days, methane emission was measured using open-circuit respirometry system and the feces and urine were collected for measuring digestion and metabolism indices. The results showed as follows:1) compared with control group, the supplementation of Baclicus lincheniformis with 2.4×10⁸ and 2.4×10⁹ CFU/d significantly reduced methane emission on the basis of dry matter intake and metabolic body weight[L/(kg W^0.75·kg DMI)] by 5.18% and 9.33% (P<0.05), respectively. 2) Compared with control group, the supplementation of Baclicus lincheniformis significantly increased the apparent digestibility of dry matter, organic matter, neutral detergent fibre, acid detergent fibre (ADF, except low dose group) and nitrogen, and retained nitrogen (P<0.05), and significantly improved dietary digestible and metabolizable energy (P<0.05). In conclusion, dietary supplementation of Baclicus lincheniformis can effectively reduce methane emission of sheep, and improve the digestibility of nutrients, all together improve utilization efficiency of dietary energy.

Key words： probiotic; Baclicus lincheniformis; methane; digestion and metabolism; mutton sheep

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