研究简报 Short Communications

早期断奶对仔猪空肠和回肠兴奋性氨基酸载体1表达的影响

  • 崔一喆 ,
  • 王秋菊 ,
  • 苏景 ,
  • 李悦 ,
  • 周亚强 ,
  • 陈玲
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  • 1. 黑龙江八一农垦大学动物科技学院, 大庆 163319;
    2. 东北农业大学动物医学学院, 哈尔滨 150030;
    3. 黑龙江省动物疫病预防与控制中心, 哈尔滨 150069

收稿日期: 2015-09-01

  网络出版日期: 2016-02-19

基金资助

黑龙江省自然科学基金项目(C201444,C2015040);黑龙江八一农垦大学校内课题资助(XZR2014-05);中国博士后科学基金项目(2013M531011)

Effects of Early Weaning on Jenunal and Ileal Excitary Amino Acid Aarrier 1 Expression of Piglets

  • CUI Yizhe ,
  • WANG Qiuju ,
  • SU Jing ,
  • LI Yue ,
  • ZHOU Yaqiang ,
  • CHEN Ling
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  • 1. College of Animal Science and Veterinary, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
    2. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;
    3. Heilongjiang Province Animal Epidemic Prevention and Control Center, Harbin 150069, China

Received date: 2015-09-01

  Online published: 2016-02-19

摘要

本试验旨在研究仔猪出生后10~20 d,早期断奶仔猪小肠谷氨酸转运载体基因表达情况与哺乳仔猪的差异。试验分别从40头不同母猪的仔猪中各选出体重相近,10日龄的"杜×长×大"三元杂交仔猪1头,共40头仔猪,随机不配对分为2组,每组20头仔猪,对照组(哺乳组)为哺乳仔猪,随母猪喂养;试验组(断奶组)为断奶仔猪,隔离断奶饲养;试验期10 d。饲养结束,每组随机取12只仔猪,宰杀取空肠和回肠,测定谷氨酸转运载体兴奋性氨基酸转运载体1(EAAC1)蛋白质表达情况和游离氨基酸含量。结果显示,断奶显著降低了仔猪空肠和回肠EAAC1(57和73 ku)及其相关蛋白谷氨酸转运联合蛋白(GTRAP3-18)(50 ku)的蛋白质和mRNA表达量(P<0.05)。断奶提高了仔猪空肠游离谷氨酸和总氨基酸含量,却降低了仔猪回肠游离谷氨酸和总氨基酸含量,差异显著(P<0.05)。结果提示,早期断奶降低EAAC1和GTRAP3-18的蛋白质含量,这可能与早期断奶仔猪遭受营养谷氨酸缺乏导致的肠道氨基酸吸收转运障碍有关。

本文引用格式

崔一喆 , 王秋菊 , 苏景 , 李悦 , 周亚强 , 陈玲 . 早期断奶对仔猪空肠和回肠兴奋性氨基酸载体1表达的影响[J]. 动物营养学报, 2016 , 28(2) : 555 -563 . DOI: 10.3969/j.issn.1006-267x.2016.02.030

Abstract

This study was conducted to examine the effects of early weaning on adaptive changes in glutamate transporter gene expression in comparison with the suckling counterpart in the pig during the ages of 10 to 20 days. A total of 40 ternary hybrid piglets, with a similar body weight randomly taken from 40 different sows at 10 days of age, were divided into 2 groups. The trial group were 20 suckling piglets randomly obtained from 20 different sow litters, were allowed to continue suckling with their sows as the suckling group for 10 days; the 20 piglets of the weaning group were taken from another 20 different sow litters, moved off-site and weaned on a weaning diet for 10 days according to standard swine industry early weaning practices. At the end of the trial, 12 piglets were randomly taken from each group to remove the jejunum and ileum, and gene expression of excitatory amino acid carrier 1 (EAAC1) and free amino acids content were determined. The results showed that weaning significantly decreased the abundance of the EAAC1 (57 and 73 ku) and glutamate transporter associate protein 3-18 (GTRAP3-18) (50 ku) protein and mRNA expression in jejunum and ileum of piglets (P<0.05). Weaning significantly increased the contents of glutamate and total free amino acids (P<0.05), while the contents of glutamate and total free amino acids in ileum were significantly decreased (P<0.05). In conclusion, early weaning decrease jejunal and ileal EAAC1 and GTRAP3-18 protein expression and this may, in part, is related to early weaned piglets suffered from lack of nutrition leading to intestinal glutamate uptake disorder.

参考文献

[1] RUTH M R,FIELD C J.The immune modifying effects of amino acids on gut-associated lymphoid tissue[J].Journal of Animal Science and Biotechnology,2013,4:27.
[2] BIANCHI M G,BARDELLI D,CHIU M,et al.Changes in the expression of the glutamate transporter EAAT3/EAAC1 in health and disease[J].Cellular and Molecular Life Sciences,2014,71(11):2001-2015.  
[3] AOYAMA K,NAKAKI T.Neuroprotective properties of the excitatory amino acid carrier 1(EAAC1)[J].Amino Acids,2013,45(1):133-142.  
[4] BUTCHBACH M E R,LAI L,LIN C L G.Molecular cloning,gene structure,expression profile and functional characterization of the mouse glutamate transporter (eAAT3) interacting protein GTRAP3-18[J].Gene,2002,292(1/2):81-90.
[5] AOYAMA K,NAKAKI T.Inhibition of GTRAP3-18 may increase neuroprotective glutathione (GSH) synthesis[J].International Journal of Molecular Sciences,2012,13(9):12017-12035.
[6] BERMAN A E,CHAN W Y,BRENNAN A M,et al.N-acetylcysteine prevents loss of dopaminergic neurons in the EAAC1-/- mouse[J].Annals of Neurology,2011,69(3):509-520.  
[7] DUERSON K,WOLTJER R L,MOOKHERJEE P,et al.Detergent-insoluble EAAC1/EAAT3 aberrantly accumulates in hippocampal neurons of Alzheimer's disease patients[J].Brain Pathology,2009,19(2):267-278.  
[8] FU D Z,YANG H S,KONG X F,et al.Molecular cloning and expression profiling of excitatory amino acid carrier 1 in suckling Huanjiang mini-piglets with large or small body weight at birth[J].Molecular Biology Reports,2013,40(4):3341-3350.  
[9] TAKITA M,KIKUSUI T.Early weaning influences short-term synaptic plasticity in the medial prefrontal-anterior basolateral amygdala pathway[J].Neuroscience Research,2015,doi:10.1016/j.neures.2015.08.003.in press
[10] XIONG X,YANG H S,TAN B,et al.Differential expression of proteins involved in energy production along the crypt-villus axis in early-weaning pig small intestine[J].American Journal of Physiology-Gastrointestinal and Liver Physiology,2015,309(4):G229-G237.
[11] CANDEIAS E M,SEBASTIÃO I C,CARDOSO S M,et al.Gut-brain connection:the neuroprotective effects of the anti-diabetic drug liraglutide[J].World Journal of Diabetes,2015,6(6):807-827.  
[12] GU X H,LI D F,SHE R P.Effect of weaning on small intestinal structure and function in the piglet[J].Archiv für Tierernaehrung,2002,56(4):275-286.
[13] WATABE M,AOYAMA K,NAKAKI T.A dominant role of GTRAP3-18 in neuronal glutathione synthesis[J].The Journal of Neuroscience,2008,28(38):9404-9413.  
[14] LIN C I,ORLOV I,RUGGIERO A M,et al.Modulation of the neuronal glutamate transporter EAAC1 by the interacting protein GTRAP3-18[J].Nature,2001,410(6824):84-88.  
[15] BURRIN D G,Stoll B.Metabolic fate and function of dietary glutamate in the gut[J].American Journal of Clinical Nutrition,2009,90(3):850S-856S.
[16] FAN M Z,MATTHEWS J C,ETIENNE N M P,et al.Expression of apical membrane L-glutamate transporters in neonatal porcine epithelial cells along the small intestinal crypt-villus axis[J].American Journal of Physiology Gastrointestinal and Liver Physiology,2004,287(2):G385-G398.
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