β-胡萝卜素对妊娠母猪粪便、血清和乳汁中免疫球蛋白A浓度的影响

张晓音; 吉昱斌; 李雁强; 吴旻; 马思慧; 郑鑫

doi:10.3969/j.issn.1006-267x.2016.02.032

动物营养学报 >

2016 , Vol. 28 >Issue 2: 572 - 578

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.032

研究简报 Short Communications

β-胡萝卜素对妊娠母猪粪便、血清和乳汁中免疫球蛋白A浓度的影响

张晓音 ,
吉昱斌 ,
李雁强 ,
吴旻 ,
马思慧 ,
郑鑫

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吉林农业大学动物科学技术学院, 长春 130118

收稿日期: 2015-08-12

网络出版日期: 2016-02-19

基金资助

国际科技合作项目(20130413037GH)

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Effects of β-Carotene on Immunoglobulin A Concentration in Feces, Serum and Milk of Late-Pregnancy Sows

ZHANG Xiaoyin ,
JI Yubin ,
LI Yanqiang ,
WU Min ,
MA Sihui ,
ZHENG Xin

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College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China

Received date: 2015-08-12

Online published: 2016-02-19

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摘要

本试验旨在研究妊娠母猪后期饲粮中添加β-胡萝卜素对妊娠母猪粪便中β-胡萝卜素含量及粪便、血清和乳汁中免疫球蛋白A(IgA)浓度的影响。将体况相近、胎次相同的妊娠母猪30头随机分成3组,每组10头猪,饲养60 d。对照组饲喂基础饲粮,半月组和一月组分别在预产期前半个月和1个月的基础饲粮中添加200 mg/kg β-胡萝卜素。用高效液相色谱法测定各组粪便中β-胡萝卜素含量,酶联免疫吸附试验(ELISA)测定粪便、血清和乳汁中IgA浓度。结果表明:半月组和一月组粪便中β-胡萝卜素含量高于对照组,大部分差异显著(P<0.05);在分娩前1周,半月组和一月组粪便中IgA浓度大部分高于对照组,但在分娩后1周,半月组和一月组粪便中IgA大部分浓度低于对照组;半月组和一月组血清和乳汁中IgA浓度高于对照组,除一月组血清IgA浓度外均差异不显著(P>0.05)。由此可见,饲粮添加β-胡萝卜素可以增加妊娠母猪粪便中β-胡萝卜素含量,提高血清和乳汁中IgA浓度,从而增强妊娠母猪的免疫功能。

关键词： β-胡萝卜素; 妊娠母猪; 高效液相色谱; 免疫球蛋白A

本文引用格式

张晓音 , 吉昱斌 , 李雁强 , 吴旻 , 马思慧 , 郑鑫 . β-胡萝卜素对妊娠母猪粪便、血清和乳汁中免疫球蛋白A浓度的影响[J]. 动物营养学报, 2016 , 28(2) : 572 -578 . DOI: 10.3969/j.issn.1006-267x.2016.02.032

Abstract

This experiment was conducted to study the effects of dietary β-carotene on fecal β-carotene content and immunoglobulin A (IgA) concentration in feces, serum and milk of late-pregnancy sows. A total of 30 sows with the similar reproductive condition and the same parity were randomly divided into 3 groups with 10 pigs per group, which were fed for 60 days. The control group was fed a basal diet, and the half month group and one month group were fed the basal diet supplemented with 200 mg/kg β-carotene before expected date half month and one month, respectively. The fecal β-carotene concentration was tested by high performance liquid chromatography (HPLC), and enzyme-linked immunosorbent assay (ELISA) was used to detect the concentration of IgA in feces, serum and milk. The results showed that the most of fecal β-carotene contents in the half month group and one month group were significantly higher than those in control group (P<0.05). A week before delivery, the most of IgA concentrations in feces in half month group and one month group were higher than those in control group; but in one week after delivery, the most of IgA concentrations in feces in half month group and one month group were lower than those in control group. In serum and milk, the IgA concentrations in the half month group and one month group were higher than those in control group except serum IgA concentration in one month group (P>0.05). In summary, β-carotene can increase fecal β-carotene content and IgA concentration in serum and milk, and then enhance immune the function of late-pregnancy sows.

Key words： β-carotene; late-pregnancy sows; HPLC; immunoglobulin A

参考文献

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